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Genome-wide CRISPR-Cas9 screens have untangled regulatory networks driving diverse biological processes. Their success relies on interrogating specific molecular phenotypes and distinguishing key regulators from background effects. Here, we realize these goals by optimizing CRISPR interference with barcoded expression reporter sequencing (CiBER-seq) to dramatically improve the sensitivity and scope of genome-wide screens. We systematically address technical factors that distort phenotypic measurements by normalizing expression reporters against closely matched promoters. We use our improved CiBER-seq to accurately capture known components of well-studied RNA and protein quality control systems. These results demonstrate the precision and versatility of CiBER-seq for dissecting cellular pathways.

El artículo presenta el análisis psicológico del proceso de socialización cibernética entre los estudiantes modernos. Las principales fuentes de investigación empírica son los resultados de la percepción subjetiva de los estudiantes de los diagnósticos de actividad en el espacio de información y comunicación de Internet y el análisis de datos sobre los síntomas del estrés de la información debido al uso activo de los recursos cibernéticos por parte de los estudiantes. The article presents the psychological analysis of cyber socialization process among modern students. The main sources of empirical research are the results of student subjective perception of activity diagnostics in the information and communication space of Internet and the analysis of data on the symptoms of information stress due to the active use of cyber-resources by students.

El artículo presenta el análisis psicológico del proceso de socialización cibernética entre los estudiantes modernos. Lasprincipales fuentes de investigación empírica son los resultados de la percepción subjetiva de los estudiantes de los diagnósticos de actividad en el espacio de información y comunicación de Internet y el análisis de datos sobre los síntomas del estrés dela información debido al uso activo de los recursos cibernéticos por parte de los estudiantes.

Objective: We aimed to map attitudes underlying complementary and alternative medicine (CAM) use, especially those involved in “dysfunctional CAM reliance,” that is, forgoing biomedical treatment in a life-threatening situation in favor of alternative treatment. Analyses of modifiable determinants of CAM use were conducted at a sufficiently specific level to inform intervention development. Methods: We collected usable data on CAM-related attitudinal beliefs from 151 participants in Budapest with varying degrees of CAM use, which we analyzed using confidence interval–based estimation of relevance plots. Results: Although there were beliefs that the entire sample shared, there was a marked difference between the biomedical and CAM groups. These differences were beliefs concerning trust in various medical systems, the level of importance assigned to emotions in falling ill, and vitalism or Eastern concepts. Regarding CAM users in general, the most successful intervention targets are beliefs in vitalism on the one hand, and distrust in biomedicine on the other. In addressing dysfunctional CAM use specifically, the most significant beliefs pertain to “natural” cures and reliance on biomedical testing. Conclusions: Albeit much research has been carried out on the motivations behind CAM use, rarely do studies treat CAM users separately in order to scrutinize patterns of nonconventional medicine use and underlying cognition. This is the first study to begin pinpointing specific attitudes involved in dysfunctional CAM use to inform future intervention development. Such interventions would be essential for the prevention of incidents and mortality.

A raíz de los esfuerzos de diferentes organizaciones internacionales, el child grooming es considerado un delito en diferentes legislaciones alrededor del mundo, aun así, en Colombia la consolidación de esta figura permanece en desarrollo sin suficientes aportes jurisprudenciales o doctrinales. Este aporte busca analizar los lineamientos generales de esta figura y adicionalmente propone algunos cambios en la legislación penal, con el propósito de sancionar algunas conductas de abuso sexual virtual, realizadas contra niños, niñas y adolescentes.

Background Antenatal care is imperative for adolescents and young women, due to their increased risk of pregnancy-related complications. Evidence on the psychosocial determinants of antenatal attendance among this vulnerable group is lacking. This study assessed the relevance of the psychosocial sub-determinants of intention and self-efficacy to attend antenatal appointments among pregnant adolescents and young women in Cape Town, South Africa; with a view to informing behaviour change interventions. Methods Pregnant women and girls aged 13-20 years were recruited to complete a cross-sectional questionnaire assessing their pregnancy experiences, pregnancy-related knowledge and psychosocial determinants related to antenatal care seeking. Confidence Interval Based Estimation of Relevance (CIBER) analysis was used to examine the association of the psychosocial sub-determinants with the intention and self-efficacy to attend antenatal appointments, and to establish their relevance for behaviour change interventions. The psychosocial sub-determinants comprised knowledge, risk perceptions, and peer, partner, family and individual participant attitudes. Results The mean gestation age of participants (n=575) was 18.7 weeks, and the mean age was 18 years. Risk perceptions of experiencing preeclampsia and heavy bleeding during pregnancy or childbirth if clinic appointments are not attended had moderate mean scores and were positively correlated with intention and self-efficacy, which makes them relevant intervention targets. Several family, peer, partner and individual participant attitudes that affirmed timely appointment attendance had strong positive associations with intention and self-efficacy but their mean score were already high. Conclusions Given the high means of the family, peer, partner and individual participant attitudes, the relevance of these attitudinal items as intervention targets was relatively low. Further studies are recommended to assess the relevance of these sub-determinants in similar populations.

Background/Objectives: Individuals diagnosed with substance use disorders (SUD) exhibit notable deficits in executive function (EFs). Notably, the pro-inflammatory cytokine interleukin-6 (IL-6) has been associated with cognitive impairments in individuals with substance use disorders. The specific neuropsychological parameters most affected by executive dysfunction remain poorly understood. Methods: In this study, sixteen patients diagnosed with SUD in the withdrawal phase were compared to twenty age-matched control subjects to ascertain which aspects of EFs were most adversely impacted. Plasma levels of IL-6 were quantified using enzyme-linked immunosorbent assay (ELISA). Data were analyzed using the Confidence Interval-Based Estimation of Relevance (CIBER) model to determine the most sensitive executive performance indicators. Results: Findings from the CIBER analysis revealed that the Wisconsin Card Sorting Test yielded the most pronounced cognitive discrepancies between males with and without SUD diagnoses. Elevated levels of IL-6 and associated executive dysfunction were observed to persist in males with SUD throughout the withdrawal phase. Conclusions: Notably, cognitive flexibility emerged as the most sensitive parameter indicative of executive dysfunction, suggesting its potential utility in tailoring clinical interventions for SUD patients during this critical recovery period.

Background There is limited information about the psychosocial sub-determinants regarding the use of HIV Testing and Counselling (HTC) services among suspected Tuberculosis (TB) patients in Sudan. This study aimed to assess the association between psychosocial beliefs and the intention to use HTC services and to establish the relevance of these beliefs for developing behaviour change interventions among suspected TB patients. Methods Suspected TB patients ( N  = 383) from four separate TB facilities completed a cross-sectional questionnaire which was based on the Reasoned Action Approach theory. Eligibility criteria included attending Tuberculosis Management Units in Kassala State as suspected TB patients and aged 18–64 years. A Confidence Interval Based Estimation of Relevance (CIBER) analysis approach was employed to investigate the association of the beliefs with the intention to use HTC services and to establish their relevance to be targeted in behaviour change interventions. Results The CIBER results showed the beliefs included in the study accounted for 59 to 70% of the variance in intention to use HTC services. The belief “My friends think I have to use HTC services” was positively associated with the intent to use HTC, and it is highly relevant for intervention development. The belief “I would fear to be stigmatized if I get a HIV positive result” was negatively related to the intention to use HTC services and was considered a highly relevant belief. The belief “If I use HTC services, health care providers will keep my HIV test result confidential” was strongly associated with the intention to use HTC services. However, the relevance of this belief as a target for future interventions development was relatively low. Past experience with HTC services was weakly associated with the intention to use HTC services. Conclusion The intention to use HTC was a function of psychosocial beliefs. The beliefs investigated varied in their relevance for interventions designed to encourage the use of HTC services. Interventions to promote intention to use HIV testing and counselling services should address the most relevant beliefs (sub-determinants). Further study is needed to establish the relevance of sub-determinants of the intention to use HTC services for interventions development.

Violence against women and girls (VAWG) is not a recent phenomenon. What is new is the additional increasing threats that millions of women and girls face because of the rapid spread of ICTs and the expansion of social media. Cases of VAWG wherein ICT and social media are used as platforms by cybercriminals can be seen in the news media coverage. This study aims to understand and determine the trend and the state of cyber VAWG to raise awareness through mining online news websites. News articles were scraped from popular news websites between 2015 to 2020. The preprocessed articles (N=3,506) were analyzed by year using the Topic Keyword Model (TKM). It was observed that the cyber VAWG articles topic trends are increasing with most of the articles focusing on the topics \"Online sexual exploitation and sexual abuse of children\" and \"ICT-related violations of privacy\". Text mining methods may address the limitations of traditional qualitative approaches. Understanding the cyber VAWG issues by mining news articles is a novel approach that could help create programs and policies to address this societal concern. Additional studies should be conducted related to sentiment analysis of news data to verify and measure the influence of cyber VAWG-related topics.

This quantitative-based research determined whether the routine activity theory influences cyber victimization. To measure the dimensions of the theory, defined as exposure to a motivated offender, suitable online target, and absence of a capable guardian, a valid and reliable questionnaire was used. The cyber victimization questionnaire developed by Álvarez-García, Dobarro, and Núñez was applied to 1,285 students selected at random from schools in Colombia. Findings: 46% are identified as exposed to a motivated offender, 37.5% are suitable online targets, and 29.8% have no capable guardians. The interdependence of these three elements revealed that 3.9% of students are at risk due to their routine activities, which had a significant influence on cyber victimization. It is proposed that these findings should be considered in the design of communicative and educational policies aimed at a responsible use of technologies.