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In response to the significantly reduced efficiency of magnetic coupling wireless charging for unmanned aerial vehicles (UAVs) caused by their high sensitivity to transmitter and receiver coil alignment, as well as landing point errors, a position identification method based on the detection coil-induced voltage fingerprint and embedded neural network regression is proposed. This enables position alignment through a 2D mechanical structure. Firstly, by means of an S–S compensation topology with a bipolar (BP) symmetrical four-detection-coil array deployed at the transmitter, the system effectively suppresses primary direct coupling, ensuring that the position of the receiver coil predominantly determines the detection signals. Secondly, by establishing a voltage fingerprint database during the offline stage and utilizing a multi-layer perceptron–radial basis function (MLP-RBF) regression model, the system achieves high-precision end-to-end positioning and alignment control during the online stage through induced voltage acquisition and data processing. Finally, experiments demonstrate that the proposed method achieves centimeter-level positioning accuracy, with an average error of approximately 1.2 cm and a maximum error of less than 1.8 cm, presenting excellent deployability and engineering applicability.

Wireless power transfer (WPT) has emerged as a compelling solution for delivering electrical energy without physical connectors, particularly in applications requiring reliability, mobility, or encapsulation. This work presents the modeling, simulation, construction, and experimental validation of an optimized dual-frequency WPT system using magnetically coupled resonant coils. Unlike conventional single-frequency systems, the proposed architecture introduces two independently controlled excitation frequencies applied to distinct transistors, enabling improved resonance behavior and enhanced power delivery across a range of coupling conditions. The design process integrates numerical circuit simulations in PSpice and three-dimensional electromagnetic analysis in ANSYS Maxwell 3D, allowing accurate evaluation of coupling coefficient variation, mutual inductance, and magnetic flux distribution as functions of coil geometry and alignment. A sixth-degree polynomial model was derived to characterize the coupling coefficient as a function of coil separation, supporting predictive tuning. Experimental measurements were carried out using a physical prototype driven by both sinusoidal and rectangular control signals under varying load conditions. Results confirm the simulation findings, showing that specific signal periods (e.g., 8 µs, 18 µs, 20 µs, 22 µs) yield optimal induced voltage values, with strong sensitivity to the coupling coefficient. Moreover, the presence of a real load influenced system performance, underscoring the need for adaptive control strategies. The proposed approach demonstrates that dual-frequency excitation can significantly enhance system robustness and efficiency, paving the way for future implementations of self-adaptive WPT systems in embedded, mobile, or biomedical environments.

• Plants possess hundreds of intracellular immune receptors encoding nucleotide-binding domain leucine-rich repeat (NLR) proteins. Full-length NLRs or a specific domain of NLRs often induce plant cell death in the absence of pathogen infection. • In this study we used genome-wide transient expression analysis to identify a group of NLRs (ANLs; ancient and autonomous NLRs) carrying autoactive coiled-coil (CCA) domains in pepper (Capsicum annuum). CCA-mediated cell death mimics hypersensitive cell death triggered by the interaction between NLRs and pathogen effectors. • Sequence alignment and mutagenesis analyses revealed that the intact α1 helix of CCAs is critical for both CCA- and ANL-mediated cell death. Cell death induced by CCAs does not require NRG1/ADR1 or NRC type helper NLRs, suggesting ANLs may function as singleton NLRs. We also found that CCAs localize to the plasma membrane, as demonstrated for Arabidopsis singleton NLR ZAR1. Extended studies revealed that autoactive CCAs are well conserved in other Solanaceae plants as well as in rice, a monocot plant. Further phylogenetic analyses revealed that ANLs are present in all tested seed plants (spermatophytes). • Our study not only uncovers the autonomous NLR clade in plants but also provides powerful resources for dissecting the underlying molecular mechanism of NLR-mediated cell death in plants.

In order to design a high efficiency Wireless Electric Vehicle Charging (WEVC) system, the design of the different system components needs to be optimized, particularly the design of a high-coupling, misalignment-tolerant inductive link (IL), comprising primary and secondary charging coils. Different coil geometries can be utilized for the primary and the secondary sides, each with a set of advantages and drawbacks in terms of weight, cost, coupling at perfect alignment and coupling at lateral misalignments. In this work, a Finite Element Method (FEM)-based systematic approach for the design of double-D (DD) charging coils is presented in detail. In particular, this paper studies the effect of different coil parameters, namely the number of turns and the turn-to-turn spacing, on the coupling performance of the IL at perfect alignment and at ±200 mm lateral misalignment, given a set of space constraints. The proposed design is verified by an experimental prototype to validate the accuracy of the FEM model and the simulation results. Accordingly, FEM simulations are utilized to compare the performance of rectangular, DD and DDQ coils. The FEM results prove the importance of utilizing an additional quadrature coil on the secondary side, despite the added weight and cost, to further improve the misalignment tolerance of the proposed inductive link design.

Recent major advancements in drone charging station design are related to the differences in coil design between the material (copper or aluminum) and inner thickness (diameter design) to address power transfer optimization and increased efficiency. The designs are normally challenged with reduced weight on the drone’s side, which can lead to reduced payload or misalignment position issues between receiver and transmitter, limiting the performance of wireless charging. In this work, the coil combination was tested in vertical alignment from 2 cm to 50 cm, and in lateral misalignment positions that were stretched across 2, 5, 8, 10, and 15 cm ranges. Simulated and experimental results demonstrated improved transfer distances when the drone battery load was 100 Ω. With the proposed design, the vertical transfer power that was achieved was 21.12 W, 0.460 A, with 81.5% transfer efficiency, while the maximum lateral misalignment air gap that was achieved was 2 cm with 19.22 W and 74.15% efficiency. This study provides evidence that the developed circuit that is based on magnetic resonant coupling (MRC) is an effective technique towards improving power transfer efficiency across different remote and unmanned Internet of Things (IoT) applications, including drones for radiation monitoring and smart agriculture.

We study the nonequilibrium kinetics during the coil-globule transition of a flexible polymer chain with active beads after a quench from good to poor solvent condition using molecular dynamics simulation. Activity for each bead is introduced via the well-known Vicsek-like alignment rule due to which the velocity of a bead tries to align towards the average direction of its neighbors. We investigate the role of quenching temperature with varying activity during collapse of this polymer. We find that although for lower activities the kinetics remains qualitatively similar for different temperatures, for higher activity noticeable differences can be identified.

In kinesin-3, the coiled-coil 1 (CC1) can sequester the preceding neck coil (NC) for autoinhibition, but the underlying mechanism is poorly understood. Here, we determined the structures of the uninhibited motor domain (MD)-NC dimer and inhibited MD-NC-CC1 monomer of kinesin-3 KIF13B. In the MD-NC-CC1 monomer, CC1 is broken into two short helices that unexpectedly interact with both the NC and the MD. Compared with the MD-NC dimer, the CC1-mediated integration of NC and MD not only blocks the NC dimer formation, but also prevents the neck linker (NL) undocking and the ADP release from the MD. Mutations of the essential residues in the interdomain interaction interface in the MD-NC-CC1 monomer restored the MD activity. Thus, CC1 fastens the neck domain and MD and inhibits both NC and NL. This CC1-mediated lockdown of the entire neck domain may represent a paradigm for kinesin autoinhibition that could be applicable to other kinesin-3 motors.

Wireless power transfer (WPT) technologies have been adopted by many products. The capability of charging multiple devices and the design flexibility of charging coils make WPT a good solution for charging smart garments. The use of an embroidered receiver (RX) coil makes the smart garment more breathable and comfortable than using a flexible printed circuit board (FPCB). In order to charge smart garments as part of normal daily routines, two types of wireless-charging systems operating at 400 kHz have been designed. The one-to-one hanger system is desired to have a constant charging current despite misalignment so that users do not need to pay much attention when they hang the garment. For the one-to-multiple-drawer system, the power delivery ability must not change with multiple garments. Additionally, the system should be able to charge folded garments in most of the folding scenarios. This paper analyses the two WPT systems for charging smart garments and provides design approaches to meet the abovementioned goals. The wireless-charging hanger is able to charge a smart garment over a coupling variance kmaxkmin=2 with only 21% charging current variation. The wireless-charging drawer is able to charge a smart garment with at least 20 mA under most folding scenarios and three garments with stable power delivery ability.

Transcription of transposable elements is tightly regulated to prevent genome damage. KRAB domain-containing zinc finger proteins (KRAB-ZFPs) and KRAB-associated protein 1 (KAP1/TRIM28) play a key role in regulating retrotransposons. KRAB-ZFPs recognize specific retrotransposon sequences and recruit KAP1, inducing the assembly of an epigenetic silencing complex, with chromatin remodeling activities that repress transcription of the targeted retrotransposon and adjacent genes. Our biophysical and structural data show that the tripartite motif (TRIM) of KAP1 forms antiparallel dimers, which further assemble into tetramers and higher-order oligomers in a concentration-dependent manner. Structure-based mutations in the B-box 1 domain prevent higher-order oligomerization without significant loss of retrotransposon silencing activity, indicating that, in contrast to other TRIM-family proteins, self-assembly is not essential for KAP1 function. The crystal structure of the KAP1 TRIM dimer identifies the KRAB domain binding site in the coiled-coil domain near the dyad. Mutations at this site abolished KRAB binding and transcriptional silencing activity of KAP1. This work identifies the interaction interfaces in the KAP1 TRIM responsible for self-association and KRAB binding and establishes their role in retrotransposon silencing.

Phytoplasmas are insect‐transmitted bacterial phytopathogens that secrete virulence effectors and induce changes in the architecture and defense response of their plant hosts. We previously demonstrated that the small (± 10 kDa) virulence effector SAP11 of Aster Yellows phytoplasma strain Witches' Broom (AY‐WB) binds and destabilizes Arabidopsis CIN (CINCINNATA) TCP (TEOSINTE‐BRANCHED, CYCLOIDEA, PROLIFERATION FACTOR 1 AND 2) transcription factors, resulting in dramatic changes in leaf morphogenesis and increased susceptibility to phytoplasma insect vectors. SAP11 contains a bipartite nuclear localization signal (NLS) that targets this effector to plant cell nuclei. To further understand how SAP11 functions, we assessed the involvement of SAP11 regions in TCP binding and destabilization using a series of mutants. SAP11 mutants lacking the entire N‐terminal domain, including the NLS, interacted with TCPs but did not destabilize them. SAP11 mutants lacking the C‐terminal domain were impaired in both binding and destabilization of TCPs. These SAP11 mutants did not alter leaf morphogenesis. A SAP11 mutant that did not accumulate in plant nuclei (SAP11ΔNLS‐NES) was able to bind and destabilize TCP transcription factors, but instigated weaker changes in leaf morphogenesis than wild‐type SAP11. Overall the results suggest that phytoplasma effector SAP11 has a modular organization in which at least three domains are required for efficient CIN‐TCP destabilization in plants.