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Analytical guidelines to increase the value of community science data
2021
Aim Ecological data collected by the general public are valuable for addressing a wide range of ecological research and conservation planning, and there has been a rapid increase in the scope and volume of data available. However, data from eBird or other large‐scale projects with volunteer observers typically present several challenges that can impede robust ecological inferences. These challenges include spatial bias, variation in effort and species reporting bias. Innovation We use the example of estimating species distributions with data from eBird, a community science or citizen science (CS) project. We estimate two widely used metrics of species distributions: encounter rate and occupancy probability. For each metric, we critically assess the impact of data processing steps that either degrade or refine the data used in the analyses. CS data density varies widely across the globe, so we also test whether differences in model performance are robust to sample size. Main conclusions Model performance improved when data processing and analytical methods addressed the challenges arising from CS data; however, the degree of improvement varied with species and data density. The largest gains we observed in model performance were achieved with 1) the use of complete checklists (where observers report all the species they detect and identify, allowing non‐detections to be inferred) and 2) the use of covariates describing variation in effort and detectability for each checklist. Occupancy models were more robust to a lack of complete checklists. Improvements in model performance with data refinement were more evident with larger sample sizes. In general, we found that the value of each refinement varied by situation and we encourage researchers to assess the benefits in other scenarios. These approaches will enable researchers to more effectively harness the vast ecological knowledge that exists within CS data for conservation and basic research.
Journal Article
Outstanding challenges and future directions for biodiversity monitoring using citizen science data
2023
There is increasing availability and use of unstructured and semi‐structured citizen science data in biodiversity research and conservation. This expansion of a rich source of ‘big data’ has sparked numerous research directions, driving the development of analytical approaches that account for the complex observation processes in these datasets. We review outstanding challenges in the analysis of citizen science data for biodiversity monitoring. For many of these challenges, the potential impact on ecological inference is unknown. Further research can document the impact and explore ways to address it. In addition to outlining research directions, describing these challenges may be useful in considering the design of future citizen science projects or additions to existing projects. We outline challenges for biodiversity monitoring using citizen science data in four partially overlapping categories: challenges that arise as a result of (a) observer behaviour; (b) data structures; (c) statistical models; and (d) communication. Potential solutions for these challenges are combinations of: (a) collecting additional data or metadata; (b) analytically combining different datasets; and (c) developing or refining statistical models. While there has been important progress to develop methods that tackle most of these challenges, there remain substantial gains in biodiversity monitoring and subsequent conservation actions that we believe will be possible by further research and development in these areas. The degree of challenge and opportunity that each of these presents varies substantially across different datasets, taxa and ecological questions. In some cases, a route forward to address these challenges is clear, while in other cases there is more scope for exploration and creativity.
Journal Article
DNA metabarcoding reveals metacommunity dynamics in a threatened boreal wetland wilderness
2020
The complexity and natural variability of ecosystems present a challenge for reliable detection of change due to anthropogenic influences. This issue is exacerbated by necessary trade-offs that reduce the quality and resolution of survey data for assessments at large scales. The Peace–Athabasca Delta (PAD) is a large inland wetland complex in northern Alberta, Canada. Despite its geographic isolation, the PAD is threatened by encroachment of oil sands mining in the Athabasca watershed and hydroelectric dams in the Peace watershed. Methods capable of reliably detecting changes in ecosystem health are needed to evaluate and manage risks. Between 2011 and 2016, aquatic macroinvertebrates were sampled across a gradient of wetland flood frequency, applying both microscope-based morphological identification and DNA metabarcoding. By using multispecies occupancy models, we demonstrate that DNA metabarcoding detected a much broader range of taxa and more taxa per sample compared to traditional morphological identification and was essential to identifying significant responses to flood and thermal regimes. We show that family-level occupancy masks high variation among genera and quantify the bias of barcoding primers on the probability of detection in a natural community. Interestingly, patterns of community assembly were nearly random, suggesting a strong role of stochasticity in the dynamics of the metacommunity. This variability seriously compromises effective monitoring at local scales but also reflects resilience to hydrological and thermal variability. Nevertheless, simulations showed the greater efficiency of metabarcoding, particularly at a finer taxonomic resolution, provided the statistical power needed to detect change at the landscape scale.
Journal Article
Automated development of the contrast–detail curve based on statistical low‐contrast detectability in CT images
2022
Purpose We have developed a software to automatically find the contrast–detail (C–D) curve based on the statistical low‐contrast detectability (LCD) in images of computed tomography (CT) phantoms at multiple cell sizes and to generate minimum detectable contrast (MDC) characteristics. Methods A simple graphical user interface was developed to set the initial parameters needed to create multiple grid region of interest of various cell sizes with a 2‐pixel increment. For each cell in the grid, the average CT number was calculated to obtain the standard deviation (SD). Detectability was then calculated by multiplying the SD of the mean CT numbers by 3.29. This process was automatically repeated as many times as the cell size was set at initialization. Based on the obtained LCD, the C–D curve was obtained and the target size at an MDC of 0.6% (i.e., 6‐HU difference) was determined. We subsequently investigated the consistency of the target sizes for a 0.6% MDC at four locations within the homogeneous image. We applied the software to images with six noise levels, images of two modules of the American College of Radiology CT phantom, images of four different phantoms, and images of four different CT scanners. We compared the target sizes at a 0.6% MDC based on the statistical LCD and the results from a human observer. Results The developed system was able to measure C–D curves from different phantoms and scanners. We found that the C–D curves follow a power‐law fit. We found that higher noise levels resulted in a higher MDC for a target of the same size. The low‐contrast module image had a slightly higher MDC than the distance module image. The minimum size of an object detected by visual observation was slightly larger than the size using statistical LCD. Conclusions The statistical LCD measurement method can generate a C–D curve automatically, quickly, and objectively.
Journal Article
Longitudinal Degradation of Pavement Marking Detectability for Mobile LiDAR Sensing Technology in Real-World Use
2023
Recent advancements in vehicle automation and driver-assistance systems that detect pavement markings has increased the importance of the detectability of pavement markings through various sensor modalities across weather and road conditions. Among the sensing techniques, light detection and ranging (LiDAR) sensors have become popular for vehicle-automation applications. This study used low-cost mobile multi-beam LiDAR to assess the performance of several types of pavement marking materials installed on a limited-access highway in various conditions, and quantified the degradation in detection performance over three years. Four marking materials, HPS-8, polyurea, cold plastic, and sprayable thermoplastic, were analyzed in the current study. LiDAR reflectivity data extracted from a total of 210 passes through the test sections were analyzed. A new detectability score based on LiDAR intensity data was proposed to quantify the marking detectability. The results showed that the pavement marking detectability varied across the material types over the years. The results provide guidance for selecting materials and developing maintenance schedules when marking detectability by LiDAR is a concern.
Journal Article
Deep learning-assisted ultra-fast/low-dose whole-body PET/CT imaging
2021
PurposeTendency is to moderate the injected activity and/or reduce acquisition time in PET examinations to minimize potential radiation hazards and increase patient comfort. This work aims to assess the performance of regular full-dose (FD) synthesis from fast/low-dose (LD) whole-body (WB) PET images using deep learning techniques.MethodsInstead of using synthetic LD scans, two separate clinical WB 18F-Fluorodeoxyglucose (18F-FDG) PET/CT studies of 100 patients were acquired: one regular FD (~ 27 min) and one fast or LD (~ 3 min) consisting of 1/8th of the standard acquisition time. A modified cycle-consistent generative adversarial network (CycleGAN) and residual neural network (ResNET) models, denoted as CGAN and RNET, respectively, were implemented to predict FD PET images. The quality of the predicted PET images was assessed by two nuclear medicine physicians. Moreover, the diagnostic quality of the predicted PET images was evaluated using a pass/fail scheme for lesion detectability task. Quantitative analysis using established metrics including standardized uptake value (SUV) bias was performed for the liver, left/right lung, brain, and 400 malignant lesions from the test and evaluation datasets.ResultsCGAN scored 4.92 and 3.88 (out of 5) (adequate to good) for brain and neck + trunk, respectively. The average SUV bias calculated over normal tissues was 3.39 ± 0.71% and − 3.83 ± 1.25% for CGAN and RNET, respectively. Bland-Altman analysis reported the lowest SUV bias (0.01%) and 95% confidence interval of − 0.36, + 0.47 for CGAN compared with the reference FD images for malignant lesions.ConclusionCycleGAN is able to synthesize clinical FD WB PET images from LD images with 1/8th of standard injected activity or acquisition time. The predicted FD images present almost similar performance in terms of lesion detectability, qualitative scores, and quantification bias and variance.
Journal Article
Accumulation curves of environmental DNA sequences predict coastal fish diversity in the coral triangle
by
Dejean, Tony
,
Juhel, Jean-Baptiste
,
Sugeha, Hagi Yulia
in
Animal biology
,
Biodiversity and Ecology
,
Ecology
2020
Environmental DNA (eDNA) has the potential to provide more comprehensive biodiversity assessments, particularly for vertebrates in species-rich regions. However, this method requires the completeness of a reference database (i.e. a list of DNA sequences attached to each species), which is not currently achieved for many taxa and ecosystems. As an alternative, a range of operational taxonomic units (OTUs) can be extracted from eDNA metabarcoding. However, the extent to which the diversity of OTUs provided by a limited eDNA sampling effort can predict regional species diversity is unknown. Here, by modelling OTU accumulation curves of eDNA seawater samples across the Coral Triangle, we obtained an asymptote reaching 1531 fish OTUs, while 1611 fish species are recorded in the region. We also accurately predict ( R ² = 0.92) the distribution of species richness among fish families from OTU-based asymptotes. Thus, the multi-model framework of OTU accumulation curves extends the use of eDNA metabarcoding in ecology, biogeography and conservation.
Journal Article
Rapid behavioural response of urban birds to COVID-19 lockdown
2021
Biodiversity is threatened by the growth of urban areas. However, it is still poorly understood how animals can cope with and adapt to these rapid and dramatic transformations of natural environments. The COVID-19 pandemic provides us with a unique opportunity to unveil the mechanisms involved in this process. Lockdown measures imposed in most countries are causing an unprecedented reduction of human activities, giving us an experimental setting to assess the effects of our lifestyle on biodiversity. We studied the birds' response to the population lockdown by using more than 126 000 bird records collected by a citizen science project in northeastern Spain. We compared the occurrence and detectability of birds during the spring 2020 lockdown with baseline data from previous years in the same urban areas and dates. We found that birds did not increase their probability of occurrence in urban areas during the lockdown, refuting the hypothesis that nature has recovered its space in human-emptied urban areas. However, we found an increase in bird detectability, especially during early morning, suggesting a rapid change in the birds’ daily routines in response to quieter and less crowded cities. Therefore, urban birds show high behavioural plasticity to rapidly adjust to novel environmental conditions, such as those imposed by the COVID-19.
Journal Article
A statistical approach to automated analysis of the low‐contrast object detectability test for the large ACR MRI phantom
2025
Background Regular quality control checks are essential to ensure the quality of MRI systems. The American College of Radiology (ACR) has developed a standardized large phantom test protocol for this purpose. However, the ACR protocol recommends manual measurements, which are time‐consuming, labor‐intensive, and prone to variability, impacting accuracy and reproducibility. Although some aspects of the ACR evaluation have been automated or semi‐automated, tests like low‐contrast object detectability (LCOD), remain challenging to automate. LCOD involves assessing the visibility of objects at various contrast levels. Purpose The purpose of this research is to propose and evaluate an automated approach for LCOD testing in MRI. Methods The automated Python code generates a one‐dimensional profile of image intensities along radial paths from the center of the contrast disk. These profiles are compared to templates created from the disc's geometric information using general linear model statistical tests. A total of 80 image volumes (40 T1‐ and 40 T2‐weighted) were assessed twice by two human evaluators and the proposed Python code. Results Human raters showed intra‐rater variability (Cohen's Kappa 0.941, 0.962), while the Python code exhibited perfect intra‐rater agreement. Inter‐rater agreement between the code and humans was comparable to human‐to‐human agreement (Cohen's Kappa 0.878 between the two human raters vs. 0.945, and 0.783 between the code and human raters). A stress test revealed both human raters and the code assigned higher scores to lower bandwidth images and lower scores to higher bandwidth images. Conclusion The proposed automated method eliminates intra‐rater variability and achieves strong inter‐rater agreement with human raters. These findings suggest the method is reliable and suitable for clinical settings, showing high concordance with human assessments.
Journal Article
Sensor and Regional Gradient Detectability of Distributed Systems
2021
In this paper the Regional Gradient Detectability ( R G – Detectability ) based on Sensors and Actuators Structures ( SAS ). So, we reflect a class of Distributed Parameter Systems ( DPSs ) in Sobolev Space ( SS ) such that the dynamics of the system is ruled by Strongly Continuous SemiGroup ( SCSS ). More accurately, we discover numerous outcomes connected with diverse types of structures with a view to accomplish that Regional Gradient Observability ( R G – Observabilityty ) notion. Therefore, we establish that, the existence of Gradient Detectable System ( GD – System ) is not realized in general case, but it perhaps Regionally Gradient Detectable System ( RG – Detctable System ).
Journal Article