Explore the vast range of titles available.

Background Diapause is a crucial adaptive strategy employed across numerous insect species, endowing them to survive in unfavorable environments. Helicoverpa armigera , one of the most destructive pests globally, undergoes diapause in the pupa stage, which is essential for its survival during the overwintering period and ultimately determines the following year's population density. 20E is a primary hormone that regulates the process of pupae diapause. However, a comprehensive analysis of the mechanisms by which 20E regulates the initiation and termination of diapause in H. armigera remains lacking. Results In the present study, exogenous 20E was initially administered to diapausing pupae, and the results demonstrated that 20E markedly enhanced the development and eclosion rate of diapausing pupae, indicating that 20E treatment effectively terminated the diapause of H. armigera . Subsequently, RNA-Seq was employed to construct a comprehensive transcriptome map of the 20E-induced termination of diapause. The results demonstrated that there were 2836 differentially expressed genes, including 1315 genes that were upregulated and 1521 genes that were downregulated, in the 20E injection group relative to the control group. KEGG and GO enrichment analysis showed that these genes were associated with various metabolic pathways. Moreover, additional analysis revealed that the majority of the pivotal genes associated with metabolism (including glycolysis/gluconeogenesis, glycerolipid, amino sugar and nucleotide sugar metabolism), cell signaling pathways (such as insulin, Wnt, MAPK signaling pathways), the cell cycle, and stress resistance exhibited altered expression following 20E injection. These findings suggest that 20E exerts its primary influence on metabolic processes, cell signaling pathways, cell cycle, and stress resistance during the termination of diapause. Conclusions Our study presents a systematic and comprehensive analysis of the genes associated with 20E-induced diapause termination, thereby providing a foundation for elucidating the molecular mechanism of 20E regulating diapause. Furthermore, the findings lend support to the utilization of ecdysone analogs as pesticides in diapause-based pest management.

Background Diapause is a physiological adaptation that evolves in arthropods to cope with unfavorable environmental conditions. By synchronizing their development and reproduction to seasonal variations, these animals can maximize their odds to survive adverse conditions. Among the environmental cues that trigger diapause, photoperiod is the most extensively studied. The hawthorn spider mite, Amphitetranychus viennensis , is one of the most devastating arthropod pests in fruit trees and woody ornamental plants. Overwintering females undergo a reproductive facultative diapause when A. viennensis immatures are exposed to long nights, which is consistent with the two-spotted spider mite, Tetranychus urticae . However, the molecular mechanisms underlying diapause in A. viennensis remain unexplored. Results Here, we first developed a photoperiodic diapause bioassay for A. viennensis and identified protochrysalis, a non-feeding resting stage between the larva and protonymph, as a crucial developmental stage for diapause induction. We then identified six genes related to the carotenoid pathway, including three involved in synthesis, AvPD , AvLC/PS1 , and AvLC/PS2 , and the other three associated with metabolism, AvBCO1 , AvBCO2 , and AvCYP384A1 . Phylogenetic analysis suggested that AvPD , AvLC/PS1 , and AvLC/PS2 were acquired through horizontal transfer from fungi. Finally, functional characterization using RNA interference (RNAi) demonstrated that AvPD , AvLC/PS1 , AvBCO2 , and AvCYP384A1 contribute to pigmentation, while AvPD , AvLC/PS1 , and AvBCO1 are involved in the regulation of diapause in A. viennensis . Conclusions This study not only advances our current knowledge on the molecular basis governing pigmentation and diapause, but also sheds light on the development of long-term sustainable management strategies for these polyphagous herbivores in spider mites in general and A. viennensis in particular.

Drosophila ezoana is a virilis group Drosophila species inhabiting northern latitudes. The flies enter adult reproductive diapause to survive winter upon exposure to short photoperiod conditions (short-day) over several consecutive days. Insect pre-diapause phase – the duration between the beginning of exposure to short days and expression of diapause is thought to be comprised of two distinct phases - (a) photoperiodic time measurement that detects short-days, followed by (b) physiological events leading to the expression of diapause phenotype. A short-day dependent segment of the pre-diapause phase thus approximates the process of photoperiodic time measurement. Continuous darkness has been found to be a neutral condition with respect to diapause regulation in many insect species. The effect of variable number of short-days followed by continuous darkness on diapause incidence thus allows identification of short-day dependent segment of pre-diapause phase thereby mapping the process of photo-periodic time measurement. Although, few weeks of exposure to short-days in adult stage is known to be sufficient for the expression of diapause in D. ezoana, the number of short days required for the completion of photo-periodic time measurement has never been systematically analysed. Our experiments show that continuous darkness is a neutral condition for diapause regulation also in D. ezoana. We utilized the neutral nature of continuous darkness to map the process of photoperiodic time measurement in the D. ezoana strain 124OJ8 which showed that integration of short-day photic cues over the first 10 days of pre-diapause phase is essential for diapause induction.

Background Diapause, a pivotal phase in the insect life cycle, enables survival during harsh environmental conditions. Unraveling the gene expression profiles of the diapause process helps uncover the molecular mechanisms that underlying diapause, which is crucial for understanding physiological adaptations. In this study, we utilize RNA-seq and Ribo-seq data to examine differentially expressed genes (DEGs) and translational efficiency during diapause of Asian corn borer ( Ostrinia furnacalis , ACB). Results Our results unveil genes classified as “forwarded”, “exclusive”, “intensified”, or “buffered” during diapause, shedding light on their transcription and translation regulation patterns. Furthermore, we explore the landscape of lncRNAs (long non-coding RNAs) during diapause and identify differentially expressed lncRNAs, suggesting their roles in diapause regulation. Comparative analysis of different types of diapause in insects uncovers shared and unique KEGG pathways. While shared pathways highlight energy balance, exclusive pathways in the ACB larvae indicate insect-specific adaptations related to nutrient utilization and stress response. Interestingly, our study also reveals dynamic changes in the HSP70 gene family and proteasome pathway during diapause. Manipulating HSP protein levels and proteasome pathway by HSP activator or inhibitor and proteasome inhibitor affects diapause, indicating their vital role in the process. Conclusions In summary, these findings enhance our knowledge of how insects navigate challenging conditions through intricate molecular mechanisms.

Diapause represents a major developmental switch in insects and is a seasonal adaptation that evolved as a specific subtype of dormancy in most insect species to ensure survival under unfavorable environmental conditions and synchronize populations. However, the hierarchical relationship of the molecular mechanisms involved in the perception of environmental signals to integration in morphological, physiological, behavioral, and reproductive responses remains unclear. In the bivoltine strain of the silkworm Bombyx mori, embryonic diapause is induced transgenerationally as a maternal effect. Progeny diapause is determined by the environmental temperature during embryonic development of the mother. Here, we show that the hierarchical pathway consists of a γ-aminobutyric acid (GABA)ergic and corazonin signaling system modulating progeny diapause induction via diapause hormone release, which may be finely tuned by the temperature-dependent expression of plasma membrane GABA transporter. Furthermore, this signaling pathway possesses similar features to the gonadotropin-releasing hormone (GnRH) signaling system for seasonal reproductive plasticity in vertebrates.

Seasonal and geographical adaptations in terms of obligatory embryonic diapause in the parthenogenetic stick insect, Ramulus mikado, were studied. First and second instar nymphs were collected at locations at three latitudes in Japan and reared in the laboratory under a photoperiod of 16L : 8D or 12L : 12D at 25°C. Their eggs were kept at 30°C for 30 or 60 days after oviposition, but no eggs hatched. Hatching was observed more than 100 days after transfer from 30°C to 15°C. The long period between transfer and hatching indicate that eggs in an early embryonic stage of development enter diapause at high-temperatures. The time from oviposition to hatching of eggs laid by adults that originated from the three locations kept under constant conditions between 15 and 25°C were compared. In all these experiments, eggs laid by individuals originating from high latitudes took longer to hatch. The eggs of those originating from Okayama and Ehime did not hatch at 25°C. However, more than 80% of the eggs of those that originated from the northernmost population hatched. Hatching before winter was observed when the eggs of those that originated from the northern population were placed outdoors in Okayama, even when the maternal insects were reared under long-day conditions in the laboratory. These fi ndings indicate that univoltine R. mikado enters diapause twice during embryonic development, which enables it survive adverse conditions in summer and winter, respectively. Furthermore, diapause intensity was lowest in insects that originated from the lowest latitude.

In general, the silkworm, Bombyx mori, has a diapause trait in its eggs. Therefore, transgenic silkworm can be produced by embryonic microinjection using eggs laid by a non-diapause strain in B. mori. In this study, we performed microinjection using eggs of diapause strains which have good characteristics for industrial use, such as a big cocoon, thin and smooth silk, and tolerance against disease due to the growing industrial use of transgenic silkworms. For the conversion of egg diapause traits from diapause to non-diapause types, we used anti-serum against the diapause hormone of B. mori (BmDH), which was injected into maternal pupae, producing non-diapause eggs at a high rate. Finally, we attempted microinjection using three diapause strains with different voltinism (i.e., number of generations of an organism in a year) and were able to successfully produce transgenic silkworms in all three of them, demonstrating that our method is applicable to a wide range of silkworm strains with a diapause trait.

Spongy moth ( Lymantria dispar Linnaeus) is a globally recognized quarantine leaf-eating pest. Spongy moths typically enter diapause after completing embryonic development and overwinter in the egg stage. They spend three-quarters of their life cycle (approximately nine months) in the egg stage, which requires a period of low-temperature stimulation to break diapause and continue growth and development. In this study, we explored the molecular mechanism underlying the diapause process in spongy moth. We performed bioinformatics analysis on four Asian populations of spongy moth and one Asian–European hybrid population through a transcriptome analysis combined with proteomics. The results revealed that 1,842 genes were differentially expressed upon diapause initiation, while 264 genes were identified upon diapause termination. Eight diapause-related genes were screened out from the three-level pathways that were significantly enriched by differentially expressed genes at the time of diapause and diapause termination, and the phylogenetic tree and protein three-dimensional structure model were constructed. This study elucidates the diapause mechanism of spongy moth at the gene and protein levels, providing theoretical insights into the early and precise prevention and control of spongy moth. This study can facilitate the development of an efficient, environmentally friendly control system for managing spongy moth populations in the field.

Many insects enter a state of dormancy (diapause) during winter in which they lower their metabolism to save energy. Metabolic suppression is a hallmark of diapause, yet we know little about the mechanisms underpinning metabolic suppression in winter or how it is reversed in the spring. Here, we show that metabolic suppression in dormant Colorado potato beetles results from the breakdown of flight muscle mitochondria via mitophagy. Diapausing Colorado potato beetles suppress their metabolism by 90%, and this lowered metabolic rate coincides with a similar reduction in flight muscle mitochondrial function and density. During early diapause, beetles increase the expression of mitophagy-related transcripts (Parkin and ATG5) in their flight muscle coincident with an increase in mitophagy-related structures in the flight muscle. Knocking down Parkin expression with RNA interference in diapausing beetles prevented some mitochondrial breakdown and partially restored the whole animal metabolic rate, suggesting that metabolic suppression in diapausing beetles is driven by mitophagy. In other animals and in models of disease, such large-scale mitochondrial degradation is irreversible. However, we show that as diapause ends, beetles reverse mitophagy and increase the expression of PGC1α and NRF1 to replenish flight muscle mitochondrial pools. This mitochondrial biogenesis is activated in anticipation of diapause termination and in the absence of external stimuli. Our study provides a mechanistic link between mitochondrial degradation in insect tissues over the winter and whole-animal metabolic suppression.

Numerous insect species living in temperate regions survive adverse conditions, such as winter, in a state of developmental arrest. The most reliable cue for anticipating seasonal changes is the day-to-night ratio, the photoperiod. The molecular mechanism of the photoperiodic timer in insects is mostly unclear. Multiple pieces of evidence suggest the involvement of circadian clock genes, however, their role might be independent of their well-established role in the daily oscillation of the circadian clock. Furthermore, reproductive diapause is preferentially studied in females, whereas males are usually used for circadian clock research. Given the idiosyncrasies of male and female physiology, we decided to test male reproductive diapause in a strongly photoperiodic species, the linden bug Pyrrhocoris apterus. The data indicate that reproduction is not under circadian control, whereas the photoperiod strongly determines males’ mating capacity. Clock mutants in pigment dispersing factor and cryptochrome-m genes are reproductive even in short photoperiod. Thus, we provide additional evidence of the participation of circadian clock genes in the photoperiodic time measurement in insects.