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Dietary components and changes cause shifts in the gastrointestinal microbial ecology that can play a role in animal health and productivity. However, most information about the microbial populations in the gut of livestock species has not been quantitative. In the present study, we utilized a new molecular method, bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP) that can perform diversity analyses of gastrointestinal bacterial populations. In the present study, cattle (n = 6) were fed a basal feedlot diet and were subsequently randomly assigned to 1 of 3 diets (n = 2 cows per diet). In each diet, 0, 25, or 50% of the concentrate portion of the ration was replaced with dried distillers grain (DDGS). Ruminal and fecal bacterial populations were different when animals were fed DDGS compared with controls; ruminal and fecal Firmicute:Bacteroidetes ratios were smaller (P = 0.07) in the 25 and 50% DDG diets compared with controls. Ruminal pH was decreased (P < 0.05) in ruminal fluid from cattle fed diets containing 50% compared with 0% DDGS. Using bTEFAP, the normal microbiota of cattle were examined using modern molecular methods to understand how diets affect gastrointestinal ecology and the gastrointestinal contribution of the microbiome to animal health and production.

Distillers’ grain (DG) was used as an ingredient in paperboard made in the laboratory from recycled containerboard fibers. The chemical composition and physico-chemical properties of DG fractions that had been isolated using varied screens were investigated. The effect of DG incorporation on the properties of the recycled paperboard was compared relative what was obtained with either precipitated calcium carbonate (PCC) or talc powder as fillers. The DG was found to mainly contain cellulose, hemicellulose, lignin, protein, and fat. At a filler addition level of 5%, the 100- to 140-mesh DG-filled handsheet exhibited the most satisfactory physical properties, with a tensile index of 25.4 N·m·g-1 and a ring crush index of 7.95 N·m·g-1. The strength values were generally higher than those of paper filled by PCC or talc powder at the same addition levels. The tensile index and ring crush index of hybrid-filled handsheets increased with increasing ratios of DG. The results suggest that DG can be used as a substitute for fiber content in some grades of paperboard, especially where a low-cost, bulky material could provide an advantage. Such usage of DG can resolve environmental challenges associated with storage and transportation of excess DG that is presently discarded.

Two experiments were conducted to determine the effect of corn processing method and corn wet distillers grains plus solubles (WDGS) level on steer performance and metabolism. In Exp. 1, 480 crossbred steer calves (314 ± 18 kg of BW) were used in a finishing experiment with a randomized complete block design and a 3 x 4 treatment structure. Diets were based on dry-rolled (DRC), high-moisture (HMC), or steam-flaked corn (SFC) with increasing levels of WDGS (0, 15, 27.5, or 40%; DM basis). A corn processing x WDGS level interaction (P < 0.01) was observed for ADG and G:F. Average daily gain and G:F increased linearly (P < 0.01) in steers fed DRC; ADG increased quadratically (P = 0.04) and G:F increased linearly (P = 0.02) in steers fed HMC; and ADG decreased quadratically (P = 0.02) with no change in G:F (P = 0.52) in steers fed SFC as WDGS increased. In Exp. 2, 7 ruminally fistulated steers (440 ± 41 kg of BW) were used in a 6-period crossover design with 3 x 2 factorial treatment structure. Diets were the same as those fed in Exp. 1, except they contained only 2 levels of WDGS (0 or 40% of diet DM). Total tract starch digestibility was greater (P < 0.01) for steers fed SFC than for steers fed DRC or HMC. Minimum ruminal pH was less (P < 0.01) for steers fed SFC than for steers fed HMC or DRC. Variance of ruminal pH was different among all 3 processing methods with DRC < HMC < SFC (P < 0.10). In situ 22-h DM digestibility of DRC and HMC and starch digestibility of DRC were greater (P < 0.10) in steers fed DRC compared with steers fed HMC or SFC. Steers fed 0% WDGS had less (P [less-than or equal to] 0.02) intake of DM, OM, NDF, and ether extract compared with steers fed 40% WDGS. Total tract digestibility of DM and OM was greater (P [less-than or equal to] 0.08) and digestibility of ether extract tended (P = 0.11) to be less for steers fed 0% WDGS compared with steers fed 40% WDGS. Maximum ruminal pH and pH variance were greater (P [less-than or equal to] 0.08) in steers fed 0% WDGS. A corn processing x WDGS level interaction (P = 0.09) was observed for ruminal acetate to propionate ratio (A:P). Within diets containing 0% WDGS, A:P in steers fed SFC was less (P [less-than or equal to] 0.08). In diets containing 40% WDGS, A:P was similar between processing methods and not different from the SFC with 0% WDGS. The corn processing x WDGS level interaction observed in the finishing experiment may be due to the decreased ruminal A:P in DRC and HMC diets with 40% WDGS.

Two experiments were conducted to determine the total tract digestibility of energy and the DE and ME values of 10 European wheat dried distillers grains with solubles (DDGS) fed to growing pigs and adult sows. The wheat DDGS were obtained from European ethanol plants and selected to get a large variability. One control diet, based on wheat (87.2%), soybean meal (10.0%), and minerals and vitamins, and 10 experimental diets prepared from the control diet and 25% each of the 10 sources of DDGS, were fed to 66 crossbred barrows (6 per diet) according to a factorial arrangement or 6 adult sows according to a pseudo Latin square design. Animals were placed in metabolism cages that allowed for the total, but separate, collection of feces and urine for 8 to 10 d after a 7- to 11-d adaptation period. By subtracting the contribution from the control diet in the DDGS-containing diets (i.e., difference method), N and GE digestibilities and DE and ME values for each source of DDGS were calculated. The energy digestibility in wheat DDGS averaged 66.5% (56.3 to 76.0%) and 71.2% (59.7 to 78.2%) in growing pigs and adult sows, respectively. Consequently, average (range) DE values of DDGS were 14.0 (11.8 to 16.2) and 14.9 (12.5 to 16.4) MJ/kg of DM for growing pigs and adult sows, respectively. Our data show that DE content of wheat DDGS can be predicted from their ADF content or from the lightness score (L). By excluding the dark and overheated samples (L <50) with the least energy digestibility and DE values, the average energy digestibility values were 69.5 and 74.4% in growing pigs and adult sows, respectively, with corresponding DE values of 14.6 and 15.6 MJ/kg DM, which are more representative of a well-controlled process for DDGS preparation. The negative effect of L on energy value and energy digestibility indicates that the occurrence of Maillard reactions should be reduced to maximize the energy value of wheat DDGS for pigs.

Background First generation bioethanol production utilizes the starch fraction of maize, which accounts for approximately 60% of the ash-free dry weight of the grain. Scale-up of this technology for fuels applications has resulted in a massive supply of distillers’ grains with solubles (DGS) coproduct, which is rich in cellulosic polysaccharides and protein. It was surmised that DGS would be rapidly adopted for animal feed applications, however, this has not been observed based on inconsistency of the product stream and other logistics-related risks, especially toxigenic contaminants. Therefore, efficient valorization of DGS for production of petroleum displacing products will significantly improve the techno-economic feasibility and net energy return of the established starch bioethanol process. In this study, we demonstrate ‘one-pot’ bioconversion of the protein and carbohydrate fractions of a DGS hydrolysate into C4 and C5 fusel alcohols through development of a microbial consortium incorporating two engineered Escherichia coli biocatalyst strains. Results The carbohydrate conversion strain E. coli BLF2 was constructed from the wild type E. coli strain B and showed improved capability to produce fusel alcohols from hexose and pentose sugars. Up to 12 g/L fusel alcohols was produced from glucose or xylose synthetic medium by E. coli BLF2. The second strain, E. coli AY3, was dedicated for utilization of proteins in the hydrolysates to produce mixed C4 and C5 alcohols. To maximize conversion yield by the co-culture, the inoculation ratio between the two strains was optimized. The co-culture with an inoculation ratio of 1:1.5 of E. coli BLF2 and AY3 achieved the highest total fusel alcohol titer of up to 10.3 g/L from DGS hydrolysates. The engineered E. coli co-culture system was shown to be similarly applicable for biofuel production from other biomass sources, including algae hydrolysates. Furthermore, the co-culture population dynamics revealed by quantitative PCR analysis indicated that despite the growth rate difference between the two strains, co-culturing didn’t compromise the growth of each strain. The q-PCR analysis also demonstrated that fermentation with an appropriate initial inoculation ratio of the two strains was important to achieve a balanced co-culture population which resulted in higher total fuel titer. Conclusions The efficient conversion of DGS hydrolysates into fusel alcohols will significantly improve the feasibility of the first generation bioethanol process. The integrated carbohydrate and protein conversion platform developed here is applicable for the bioconversion of a variety of biomass feedstocks rich in sugars and proteins.

The purpose of this study was to determine the lactation performance of dairy cows fed dried or wet distillers grains (DG) with solubles (DDGS or WDGS) at 2 dietary concentrations. A trial using 15 cows was designed as a replicated 5 x 5 Latin square with periods of 4 wk each and data collected during wk 3 and 4 of each period. Diets, on a dry matter basis, were: control, 10% DDGS, 20% DDGS, 10% WDGS, and 20% WDGS. All diets contained 25% corn silage, 25% alfalfa hay, and 50% of the respective concentrate mixes. Dry matter intake (DMI) tended to be greater for cows fed control than DG (23.4, 22.8, 22.5, 23.0, and 21.9 kg/d for control, 10% DDGS, 20% DDGS, 10% WDGS, and 20% WDGS). Milk yield (39.8, 40.9, 42.5, 42.5, and 43.5 kg/d) was greater for cows fed DG than control. Milk fat percentage (3.23, 3.16, 3.28, 3.55, and 3.40%) was similar for cows fed control and DG, but greater for cows fed WDGS than DDGS. Milk fat yield was greater for cows fed DG than control and tended to be greater for cows fed WDGS than DDGS. Milk fat from cows fed DG, especially 20% DG, was more unsaturated and contained more cis-9, trans-11 conjugated linoleic acid than when fed the control diet. Milk protein percentage (3.05, 3.01, 3.02, 3.11, and 3.06%) was similar for cows fed control and DG but greater for cows fed WDGS than DDGS. Milk protein yield was greater for cows fed DG than control, tended to be greater for cows fed WDGS than DDGS, and tended to be greater for cows fed 20% DG than 10% DG. Milk urea nitrogen was similar for cows fed control and DG but greater for cows fed WDGS than DDGS and tended to be higher for cows fed 20% DG than 10% DG. Ruminal ammonia concentrations were greater for cows fed WDGS than DDGS. Overall, feeding DG improved feed efficiency (1.70, 1.79, 1.87, 1.84, and 1.92 kg of energy-corrected milk/kg of DMI) by increasing yields of milk, protein, and fat while tending to decrease DMI.

Effects of monensin withdrawal and cattle subspecies on the utilization of bermudagrass hay (14.3% CP, 72.3% NDF, and 36.9% ADF) were evaluated using ruminally cannulated steers (5 Bos taurus indicus [BI] and 5 Bos taurus taurus [BT]). Subspecies were concurrently subjected to a 2-period, 2-treatment crossover design. Treatments consisted of either 0 mg·steer-1·d-1 monensin with no previous monensin feeding (CON) or withdrawal from 200 mg·steer-1·d-1 monensin (MON) fed individually in 0.91 kg dried distillers' grains with solubles for 42 d. Withdrawal was evaluated for a 28-d period. Ruminal fluid was collected 2 h after feeding on d 0, 1, 4, 7, 14, and 21 after withdrawal for determination of pH, VFA, ruminal NH3-N (RAN), rate of NH3 production, and CH4 production rate. Hay, ort, and fecal grab samples were collected d 23 through 28 after withdrawal for determination of intake and digestion. No subspecies x monensin, subspecies x day, or subspecies x monensin x day interactions were observed (P = 0.11). An effect of day after monensin withdrawal was observed (P < 0.01) for total VFA concentration, with an increase following withdrawal followed by a decrease and then stabilization. Monensin x day after monensin withdrawal interactions (P = 0.01) were observed for the acetate:propionate (A:P) ratio and molar percent of acetate and propionate. There was a decrease in molar percent of propionate between d 1 and 4 from 19.1 to 18.0; however, it remained greater (P = 0.10) for MON than CON through d 7. Withdrawal increased molar percent of acetate from 68.3 to 69.8 between d 0 and 4 for MON steers. The A:P ratio was less (P = 0.01) on d 0 for MON than for CON (3.4 vs. 4.0), but by d 4, it increased to 3.8 and was not different (P = 0.14) from CON. By d 14, no differences (P = 0.88) remained for acetate, propionate, or the A:P ratio. After monensin withdrawal, monensin reduced (P < 0.01) RAN by 12.3% (2.09 vs. 1.83 mM for CON and MON, respectively). Monensin withdrawal and cattle subspecies had no effect (P = 0.23) on rate of NH3 production or CH4 production rate. Monensin withdrawal had no effect (P = 0.45) on intake or digestibility parameters. Greater forage OM intake (P = 0.09; 21.2 vs. 19.2 g/kg BW) and OM digestibility (P < 0.01; 72.4 vs. 63.0%) resulted in greater (P < 0.01) total digestible OM intake (16.8 vs. 13.2 g/kg BW) in BT steers than in BI steers. These results suggest that BT steers are better able to utilize bermudagrass hay than BI steers. Upon monensin withdrawal, steers previously fed monensin continue to have a reduced A:P ratio for at least 7 d.

A study was conducted to determine the AA digestibility and energy concentration of a specialized high-protein corn distillers dried grains (HPC-DDG) product and a high-protein sorghum dried distillers grains with solubles (HPS-DDGS) product. Six growing barrows (BW = 22.7 kg) were surgically fitted with T-cannulas at the terminal ileum and allotted randomly to 3 treatments in a crossover design with 3 periods. The treatment diets were 1) 67% HPC-DDG and 2) 50% HPS-DDGS as the sole protein sources, and 3) an N-free diet for determining basal endogenous AA loss. All diets contained 0.25% chromic oxide as an inert marker. Digesta and fecal samples were collected and analyzed for AA and energy concentrations. After chemical analysis, standardized and apparent ileal digestible (SID and AID, respectively) AA and GE were determined for each coproduct. The DE, ME, and NE values for HPC-DDG and HPS-DDGS also were calculated. The chemical composition of HPC-DDG and HPS-DDGS on a DM basis was 40.8% CP, 5.4% fat, 22.9% ADF, 36.6% NDF, 0.04% Ca, and 0.42% P and 48.2% CP, 3.1% fat, 17.5% ADF, 20.4% NDF, 0.13% Ca, and 0.82% P, respectively. The DM content of HPC-DDG and HPS-DDGS was 89.50 and 91.88%, respectively. Analyzed AA content of HPC-DDG was greater than that of traditional corn DDGS. The Lys content of HPC-DDG was 1.36% (DM basis), resulting in a Lys-to-CP ratio of 3.2%. In HPS-DDGS, most AA were present in greater proportions than in HPC-DDG or conventional sorghum DDGS. The HPS-DDGS Lys content was 1.7% (DM basis), equivalent to a Lys-to-CP ratio of 3.5%. In HPC-DDG, the AID for Lys, Met, Thr, and Trp were 65.9 ± 1.7, 87.0 ± 1.9, 72.8 ± 3.4, and 76.2% ± 3.5, respectively, and SID values were 67.8 ± 1.7, 87.5 ± 1.9, 75.0 ± 3.5, and 78.6 ± 3.7%, respectively. For HPS-DDGS, the AID for Lys, Met, Thr, and Trp were 51.9 ± 5.3, 73.0 ± 3.1, 60.6 ± 5.3, and 71.7 ± 3.4%, respectively, and SID values were 53.7 ± 4.9, 73.8 ± 3.0, 63.0 ± 4.9, and 73.8 ± 3.0%, respectively. The GE, DE, and calculated ME and NE values were 5,293, 3,703 ± 121, 3,426 ± 121, and 2,131 ± 88 kcal/kg of DM, respectively, for HPC-DDG and 5,108, 3,878, 3,549, and 2,256 kcal/kg of DM, respectively, for HPS-DDGS. Results indicate that both coproducts are well suited for use in swine diets and that actual AA digestibility values and calculated energy concentrations can now be used in swine diet formulation.

Distillers dried grains with solubles (DDGS) may be included in diets fed to pigs in all phases of production. The concentrations of DE and ME in DDGS are similar to those in corn. Phosphorus in DDGS is highly digestible to pigs and apparent total tract digestibility values of approximately 60% have been reported. The concentration of starch in DDGS is low (i.e., between 3 and 11%), but the concentration of fat in DDGS is approximately 10% and the concentrations of ADF, NDF, and total dietary fiber in DDGS are approximately 3 times greater than those in corn (9.9, 25.3, and 42.1%, respectively). The apparent total tract digestibility of dietary fiber is less than 50%, which results in reduced digestibility values for DM and energy in DDGS. The concentrations of most AA in DDGS are approximately 3 times greater than those in corn, but the standardized ileal digestibility of most AA is approximately 10 percentage units less than in corn. Nursery pigs from 2 to 3 wk postweaning, and growing and finishing pigs may be fed diets containing up to 30% DDGS without any negative impact on growth performance. However, the carcass fat in pigs fed diets containing DDGS has a greater iodine value than the carcass fat in pigs not fed DDGS. It may, therefore, be necessary to withdraw DDGS from the diet of finishing pigs during the final 3 to 4 wk before slaughter to achieve the desired pork fat quality. Lactating sows can also be fed diets containing up to 30% DDGS, and DDGS can replace all the soybean meal in diets fed to gestating sows without negatively affecting sow or litter performance. Inclusion of DDGS in diets fed to pigs may improve immune system activation, but more research is needed to elucidate the mechanisms responsible for these effects. Manure volume will increase when DDGS is included in the diets because of the reduced digestibility of DM in DDGS. Nitrogen excretion may also increase, but this can be prevented by the use of crystalline AA in diets containing DDGS. In contrast, P excretion can be reduced in diets containing DDGS if the total dietary concentration of P is reduced to compensate for the greater digestibility of P in DDGS. In conclusion, DDGS can be included in diets fed to growing pigs in all phases of production, beginning at 2 to 3 wk postweaning, in concentrations of up to 30% DDGS, and lactating and gestating sows can be fed diets containing up to 30 and 50%, respectively, without negatively affecting pig performance.

This article contains the results of the evaluation of distillers grain (DG) coproducts from different ethanol plants around the United States and supplemented in animal diets in Texas, based on samples analyzed from 2008 to 2014. The samples were assessed for concentration, occurrence, and prevalence of selected nutrients and contaminants. Protein and sulfur contents of DG were largely different between maize and sorghum coproducts, as well as wet distillers grain with solubles (WDGS) and dried distillers grain with solubles (DDGS), indicating a significant effect of grain feedstock and dry grind process stream on DG composition and quality. Salmonella was isolated in 4 DDGS samples of a total of 157 DG samples, a percentage (2.5%) that is lower than the percentage of Salmonella -positive samples found in other feed samples analyzed during the same period. A small amount of virginiamycin residue was found in 24 maize DDGS, 1 maize WDGS, and 2 sorghum DDGS samples of 242 samples in total. One sorghum DDGS sample of 168 DG samples was contaminated with animal protein prohibited for use in ruminant feed and was channeled to poultry feed. The concentrations of aflatoxin and fumonisin DG coproducts averaged 3.4 μg/kg and 0.7 mg/kg, respectively. Among contaminated maize DG samples, five DDGS samples for aflatoxin contained a higher concentration than the U.S. Food and Drug Administration (FDA) minimum action level of 20 μg/kg for use in animal feed, whereas no sample for fumonisin was found above the action level of 5 mg/kg. The study provides the most current results involving DG coproducts and associated hazards that will assist development of food safety plans required by the FDA in their September 2015 rule titled \"Current Good Manufacturing Practice Hazard Analysis and Risk Based Preventive Controls for Food for Animals.\"