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Cellular growth and the preparation of cells for division between two successive cell divisions is called the cell cycle. The cell cycle is divided into several phases; the length of these particular cell cycle phases is an important characteristic of cell life. The progression of cells through these phases is a highly orchestrated process governed by endogenous and exogenous factors. For the elucidation of the role of these factors, including pathological aspects, various methods have been developed. Among these methods, those focused on the analysis of the duration of distinct cell cycle phases play important role. The main aim of this review is to guide the readers through the basic methods of the determination of cell cycle phases and estimation of their length, with a focus on the effectiveness and reproducibility of the described methods.

Indonesia is striving to meet its growing electricity demands in a sustainable way, by moving away from oil and gas sources. The government, particularly in East Kalimantan, is considering the repurposing of the offshore facilities for Ocean Thermal Energy Conversion (OTEC), which not only provides a clean and sustainable electricity, but also offers additional benefits such as agricultural and aquaculture products. This study will look at how OTEC may be integrated into an edu-ecotourism concept, which aims to promote renewable energy technology and create a sustainable economy that benefits local communities and stakeholders. To meet the study's aims, research of the possible use of OTEC as an edu-ecotourism attraction will be conducted, through papers that are reviewed from previous studies to further the understanding and the potential implementation of OTEC for edu-ecotourism. The OTEC itself is planned to have the capability of producing around 20 kW of net power. Therefore, analysis and review of potential uses and benefit of OTEC is crucial to integrate the multi-functionality of OTEC to cater not just to the goal of creating an edu-ecotourism attraction, but also to the various needs of the various stakeholders, that have a part or affected by this project.

The Edu-Metaverse, a vast ensemble of different technologies, has initiated a great and unprecedented change in the field of education. This change has been effected through the following Edu-Metaverse characteristics: embodied and multimodal interaction; immersive teaching scenarios, which can accelerate learning and skill acquisition; and the emergence of AI-enabled agents. In comparison to traditional classroom teaching models, smart education is a collaborative and visual model that adopts the latest AI technologies to reach a learning outcome. However, a problem that should be considered is how a smart education model, enabled by the Edu-Metaverse, can be developed to enhance better learning outcomes for students. Such a model should highlight smart pedagogy in the context of the Edu-Metaverse, together with a smart teaching environment, multimodal teaching resources, and AI-enabled assessment. In this study, we focused on the teaching of college English to 60 students from Zhejiang Open University. We investigated the effectiveness of a smart education model, which was empowered by the Edu-Metaverse, in enhancing better learning outcomes for the students, using a combination of qualitative and quantitative research. After the one-semester-long experiment, questionnaires were sent out to complement the interview findings. It was found that the students who engaged in the smart education model in the Edu-Metaverse yielded higher scores in oral English, vocabulary and grammar, reading comprehension, English-to-Chinese translation, and writing than those who engaged in traditional instruction. Therefore, this study suggests that a smart education model enabled by the Edu-Metaverse, which is characterized by a highly immersive experience, multimodal interaction, and a high degree of freedom for resource sharing and creation can help learners to realize deep learning, develop their capacity for high-order thinking, and help them to become intelligent individuals in an online learning space. In order to facilitate this smart learning, we make the following suggestions for educational institutions: (1) teachers should improve the design of teaching scenarios, (2) teachers should focus on learning assessment that is based on core literacy, and (3) teachers’ knowledge of the architecture of the Edu-Metaverse should be enhanced.

In studies of the impact of tourism, considerable attention has been given to the attitudes and perceptions of host communities toward tourism. However, most of these studies have focused on tourism in general and on sporadic alternative tourism in particular. This study aims to fill a gap in the research literature by examining residents’ perceptions of the economic, socio-cultural, and environmental sustainability of edu-tourism. It contributes to the limited literature on edu-tourism and to that on residents’ perceptions of tourism in small island developing states. A sample of 300 (N = 300) residents was surveyed in the city of Famagusta in Northern Cyprus to investigate residents’ attitudes toward the sustainability of edu-tourism. ANOVA and Cramér’s V tests were applied to analyze the perceptions of residents of the social, economic, and environmental impacts of edu-tourism. The influence of the socio-demographic characteristics of the residents on their perceptions and attitudes was of these issues was also examined. The study revealed that there is a variety of attitudes among residents toward the sustainability of edu-tourism and that, although residents are generally positive about edu-tourism, attitudes toward its social and environmental sustainability are mixed.

Aim/Purpose: The present study explores the use of AI-powered ChatGPT as a feedback tool for automated writing evaluation in a higher education context. Background: Artificial intelligence (AI) is increasingly applied in the field of education, offering new opportunities with its evolving capabilities. One area where AI tools have promising potential is the field of second/foreign language (L2) writing. Methodology: Participants, Turkish pre-service teachers of English enrolled in an academic writing course, received feedback from ChatGPT, peers, and the course instructor. Data came from focus-group interviews focusing on the participants’ perceptions of ChatGPT as a feedback tool in comparison to peer and teacher feedback. Thematic analysis was conducted on the transcribed interview data. Contribution: Students can benefit from AI feedback by independently refining their writing. They should develop critical thinking skills when using AI tools like ChatGPT for feedback. Findings: Thematic analysis revealed that participants thought ChatGPT had several affordances, such as practicality, interactivity, and adaptability; however, it also had certain constraints, such as occasional inconsistencies and dependence on the quality of prompts. Overall, the study highlights that ChatGPT could be a valuable feedback tool for L2 writing. Recommendations for Practitioners: The findings suggest that ChatGPT can significantly facilitate the L2 writing process but cannot fully replace feedback from peers or teachers. Teachers should provide initial training and ongoing support to students in using AI effectively for feedback. Policymakers should consider the advancements in AI and their implications for L2, anticipate students’ increasing access to advanced writing assistance, and seek ways to offer suitable guidance. Future Research: This study tapped into students’ experiences as a snapshot. Future research may focus on the long-term influence of ChatGPT feedback on L2 writing and how to combine it with other types of feedback to optimize learning.

Successful germination and seedling development are crucial steps in the growth of a new plant. In this study, we investigated the course of the cell cycle during germination in relation to grain hydration in the model grass Brachypodium distachyon (Brachypodium) for the first time. Flow cytometry was performed to monitor the cell cycle progression during germination and to estimate DNA content in embryo tissues. The analyses of whole zygotic embryos revealed that the relative DNA content was 2C, 4C, 8C, and 16C. Endoreplicated nuclei were detected in the scutellum and coleorhiza cells, whereas the rest of the embryo tissues only had nuclei with a 2C and 4C DNA content. This study was accompanied by a spatiotemporal profile analysis of the DNA synthetic activity in the organs of Brachypodium embryos during germination using EdU labelling. Upon imbibition, nuclear DNA replication was initiated in the radicle within 11 h and subsequently spread towards the plumule. The first EdU-labelled prophases were observed after 14 h of imbibition. Analysis of selected genes that are involved in the regulation of the cell cycle, such as those encoding cyclin-dependent kinases and cyclins, demonstrated an increase in their expression profiles.

Thermoelectric materials, which can generate electricity from waste heat or be used as solid-state Peltier coolers, could play an important role in a global sustainable energy solution. Such a development is contingent on identifying materials with higher thermoelectric efficiency than available at present, which is a challenge owing to the conflicting combination of material traits that are required. Nevertheless, because of modern synthesis and characterization techniques, particularly for nanoscale materials, a new era of complex thermoelectric materials is approaching. We review recent advances in the field, highlighting the strategies used to improve the thermopower and reduce the thermal conductivity.

Root stem cell niche functioning requires the formation and maintenance of the specific “auxin-rich domain” governed by directional auxin transport and local auxin production. Auxin maximum co-localizes with the WOX5 expression domain in the quiescent center that separates mitotically active proximal and distal root meristems. Here we unravel the interconnected processes happening under WOX5 overexpression by combining in vivo experiments and mathematical modeling. We showed that WOX5-induced TAA1-mediated auxin biosynthesis is the cause, whereas auxin accumulation, PIN transporters relocation, and auxin redistribution between proximal and distal root meristems are its subsequent effects that influence the formation of the well-described phenotype with an enlarged root cap. These findings helped us to clarify the role of WOX5, which serves as a local QC-specific regulator that activates biosynthesis of non-cell-autonomous signal auxin to regulate the distal meristem functioning. The mathematical model with WOX5-mediated auxin biosynthesis and auxin-regulated cell growth, division, and detachment reproduces the columella cells dynamics in both wild type and under WOX5 dysregulation.

We have developed a method to detect DNA synthesis in proliferating cells, based on the incorporation of 5-ethynyl-2'-deoxyuridine (EdU) and its subsequent detection by a fluorescent azide through a Cu(I)-catalyzed [3 + 2] cycloaddition reaction (\"click\" chemistry). Detection of the EdU label is highly sensitive and can be accomplished in minutes. The small size of the fluorescent azides used for detection results in a high degree of specimen penetration, allowing the staining of whole-mount preparations of large tissue and organ explants. In contrast to BrdU, the method does not require sample fixation or DNA denaturation and permits good structural preservation. We demonstrate the use of the method in cultured cells and in the intestine and brain of whole animals.

Measurement of the direction of cell division is an important, yet difficult, task to analyse how a plant organ acquires its final shape from an initially small group of cells. We introduce a method that rapidly and easily quantifies cell division direction and is applicable to all plant species. A pulse-chase strategy for 5-ethynyl-2′-deoxyuridine (EdU) labelling assay was established and was shown to be successful for leaves of Arabidopsis thaliana (Arabidopsis) and Juncus prismatocarpus. By optimization of the pulse and chase periods, most of the signals obtained were sets of daughter nuclei. For Arabidopsis, the optimal time was a 45-min pulse and a 7-h chase. For J. prismatocarpus, the optimal time was a 2-h pulse and a 13.5-h chase. The positions of the daughter nuclei were used to quantify cell division direction in the Arabidopsis leaf primordia. Overall, cell division along the proximal–distal axis was more frequent than along the medial–lateral axis. In petiole, major vein, minor vein and margin areas, the major cell division direction seemed to be coincident with the direction of auxin flow. The advantages of our method over the few methods used previously are discussed. We anticipate that it will provide opportunities to study plant development in the near future.