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Introduction This paper explores and critiques the typographic and design decisions made in the creation of the logo and brand for the 2oo5 film Serenity by Joss Whedon and how the choice of the Papyrus-inspired typeface used in the logo perpetuates the potential for orientalist, racialized stereotypes, and material dishonesty previously seen in the film's casting, props, and story critiqued prior in Serenity scholarly literature. [...]the Serenity logo takes on six different forms (Fig. i): 1. The American Marketing Association describes a brand as a \"name, term, sign, symbol, or design, or a combination of them, intended to identify the goods and services of one seller or group of sellers and to differentiate them from those of competition\" (American Marketing Association). The Serenity logo has succeeded in creating strong brand equity as 20 years later many Browncoats still adore the TV show, film, comics, books, and merchandise as much as ever.

Background With their charismatic nighttime flashes, fireflies are a classic organismal system for studying the evolution of visual mating signals. However, across their diversity, fireflies employ a variety of mating strategies that include both chemical and visual signals. While phylogenetic evidence points to a common ancestor that relied on long-range pheromones, behavioral evidence suggests that light-dependent flashing fireflies do not use smell for mating. We tested this hypothesis by investigating the olfactory genetics of the dusk-active, light-using common eastern firefly, Photinus pyralis. In insects, odors are primarily detected by odorant receptor (OR) proteins embedded in the dendritic membranes of olfactory receptor neurons. If pheromones are part of mate signaling in light-using fireflies, then one or more OR genes should be upregulated in the antennae of the searching sex (males). We therefore annotated the complete suite of ORs in the genome of P . pyralis and measured expression of OR genes between the sexes using RNAseq. Results We identified 102 ORs in the P. pyralis genome, including the conserved single-copy Orco. Our phylogenetic analysis showed lineage-specific OR diversification in P. pyralis relative to other beetle species. Differential expression analysis of male and female antennae and hind legs revealed that a subset of ORs were upregulated in antennae as compared to legs, suggesting a role in adult olfaction. Notably, PpyrOR6 was one of two genes, and the only OR, that was significantly upregulated between male and female antennae, suggesting a sex-specific role such as mate location. Conclusions These findings increase known diversity of insect ORs in an understudied beetle family and suggest that fireflies with flashing adults use multimodal signals during mating.

Animals employ different sexual signal modes (e.g. visual, acoustic, chemical) in different environments and behavioural contexts. If sensory structures are costly, then evolutionary shifts in primary signal mode should be associated with changes in sensor morphology. Further, sex differences are expected if male and female signalling behaviours differ. Fireflies are known for their light displays, but many species communicate exclusively with pheromones, including species that recently lost their light signals. We performed phylogenetically controlled analyses of male eye and antenna size in 46 North American taxa, and found that light signals are associated with larger eyes and shorter antennae. In addition, following a transition from nocturnal light displays to diurnal pheromones, eye size reductions occur more rapidly than antenna size increases. In agreement with the North American taxa, across 101 worldwide firefly taxa in 32 genera, we found light displays are associated with larger eye and smaller antenna sizes in both males and females. For those taxa with both male and female data, we found sex differences in eye size and, for diurnal species, in antenna size.

In recent decades, advances in lighting technology have precipitated exponential increases in night sky brightness worldwide, raising concerns in the scientific community about the impact of artificial light at night (ALAN) on crepuscular and nocturnal biodiversity. Long‐term records show that insect abundance has declined significantly over this time, with worrying implications for terrestrial ecosystems. The majority of investigations into the vulnerability of nocturnal insects to artificial light have focused on the flight‐to‐light behavior exhibited by select insect families. However, ALAN can affect insects in other ways as well. This review proposes five categories of ALAN impact on nocturnal insects, highlighting past research and identifying key knowledge gaps. We conclude with a summary of relevant literature on bioluminescent fireflies, which emphasizes the unique vulnerability of terrestrial light‐based communication systems to artificial illumination. Comprehensive understanding of the ecological impacts of ALAN on diverse nocturnal insect taxa will enable researchers to seek out methods whereby fireflies, moths, and other essential members of the nocturnal ecosystem can coexist with humans on an increasingly urbanized planet. In recent decades, advances in lighting technology have precipitated exponential increases in night sky brightness worldwide, raising concerns in the scientific community about the impact of artificial light at night (ALAN) on crepuscular and nocturnal biodiversity. Long‐term records show that insect abundance has declined significantly over this time, with worrying implications for terrestrial ecosystems. This review proposes five categories of ALAN impact on nocturnal insects, highlighting past research and identifying key knowledge gaps. We conclude with a summary of relevant literature on bioluminescent fireflies, which emphasizes the unique vulnerability of terrestrial light‐based communication systems to artificial illumination.

In recent years, we face an increasing interest in protecting multimedia data and copyrights due to the high exchange of information. Attackers are trying to get confidential information from various sources, which brings the importance of securing the data. Many researchers implemented techniques to hide secret information to maintain the integrity and privacy of data. In order to protect confidential data, histogram-based reversible data hiding with other cryptographic algorithms are widely used. Therefore, in the proposed work, a robust method for securing digital video is suggested. We implemented histogram bit shifting based reversible data hiding by embedding the encrypted watermark in featured video frames. Histogram bit shifting is used for hiding highly secured watermarks so that security for the watermark symbol is also being achieved. The novelty of the work is that only based on the quality threshold a few unique frames are selected, which holds the encrypted watermark symbol. The optimal value for this threshold is obtained using the Firefly Algorithm. The proposed method is capable of hiding high-capacity data in the video signal. The experimental result shows the higher capacity and video quality compared to other reversible data hiding techniques. The recovered watermark provides better identity identification against various attacks. A high value of PSNR and a low value of BER and MSE is reported from the results.

Fireflies (Coleoptera: Lampyridae) have distinct visual systems at different stages of development. Larvae have stemmata and adults have compound eyes. Adults use compound eyes to mediate photic communication during courtship. Larvae do not manifest this behavior, yet they are bioluminescent. We investigated the structure of stemmata in Photuris firefly larvae to identify anatomical substrates (i.e., rhabdomeres) conferring visual function. Stemmata were located bilaterally on the antero-lateral surfaces of the head. Beneath the ~ 130 µm diameter lens, we identified a pigmented eye-cup. At its widest point, the eye-cup was ~ 150 µm in diameter. The optic nerve exited the eye-cup opposite the lens. Two distinct regions, asymmetric in size and devoid of pigmentation, were characterized in stemmata cross-sections. We refer to these regions as lobes. Each lobe contained a rhabdom of a radial network of rhabdomeres. Pairs of rhabdomeres formed interdigitating microvilli contributed from neighboring photoreceptor cell bodies. The optic nerve contained 88 axons separable into two populations based on size. The number of axons in the optic nerve together with distinct rhabdoms suggests these structures were formed from ‘fusion stemmata.’ This structural specialization provides an anatomical substrate for future studies of visually mediated behaviors in Photuris larvae.

Background Bioluminescence imaging (BLI) is a powerful technique for monitoring the temporal and spatial dynamics of gene expression in the mouse brain. However, the black fur, skin pigmentation and hair regrowth after depilation of mouse interfere with BLI during developmental and daily examination. The aim of this study was to extend the application of Arc - Luc transgenic (Tg) mice to the BLI of neuronal activity in the mouse brain by introducing the hairless (HL) gene and to examine Arc - Luc expression at various developmental stages without interference from black fur, skin pigmentation, and hair regrowth. Results The Arc - Luc Tg HL mice were established by crossing the Tg C57BL/6 mouse strain with the HL mouse strain. Under physiological and pathological conditions, BLI was performed to detect the signal intensity changes at various developmental stages and at an interval of <7 days. The established Arc - Luc Tg HL mice exhibited clear and stable photon signals from the brain without interference during development. After surgical monocular deprivation during visual-critical period, large signal intensity changes in bioluminescence were observed in the mouse visual cortex. Exposure of mice to a novel object changed the photon distribution in the caudal and rostral cerebral areas. The temporal pattern of kainic-acid-induced Arc - Luc expression showed biphasic changes in signal intensity over 24 h. Conclusions This study showed the advantages of using the mutant HL gene in BLI of Arc expression in the mouse brain at various developmental stages. Thus, the use of the Arc - Luc Tg HL mice enabled the tracking of neuronal-activity-dependent processes over a wide range from a focal area to the entire brain area with various time windows.

Firefly flashes are well-known visual signals used by these insects to find, identify, and choose mates. However, many firefly species have lost the ability to produce light as adults. These “unlighted” species generally lack developed adult light organs, are diurnal rather than nocturnal, and are believed to use volatile pheromones acting over a distance to locate mates. While cuticular hydrocarbons, which may function in mate recognition at close range, have been examined for a handful of the over 2000 extant firefly species, no volatile pheromone has ever been identified. In this study, using coupled gas chromatography - electroantennographic detection, we detected a single female-emitted compound that elicited antennal responses from wild-caught male winter fireflies, Photinus corruscus. The compound was identified as (1S)-exo-3-hydroxycamphor (hydroxycamphor). In field trials at two sites across the species’ eastern North American range, large numbers of male P. corruscus were attracted to synthesized hydroxycamphor, verifying its function as a volatile sex attractant pheromone. Males spent more time in contact with lures treated with synthesized hydroxycamphor than those treated with solvent only in laboratory two-choice assays. Further, using single sensillum recordings, we characterized a pheromone-sensitive odorant receptor neuron in a specific olfactory sensillum on male P. corruscus antennae and demonstrated its sensitivity to hydroxycamphor. Thus, this study has identified the first volatile pheromone and its corresponding sensory neuron for any firefly species, and provides a tool for monitoring P. corruscus populations for conservation and further inquiry into the chemical and cellular bases for sexual communication among fireflies.

Firefly algorithm (FA) is efficient in solving continuous optimal problems, because of its ability to a global search. However, the redundant attractions and incorrect directions may reduce the efficiency of FA. To improve the performance of FA, a novel multi-group mechanism is proposed based on an assumption that firefly has a visual field. The modified firefly algorithm is called the visual firefly algorithm(VFA). The framework of VFA combines the assumption with the designed strategies to balance the exploration and exploitation. Where the proposed observer strategy works for the exploration, the suggested selective random strategy plays the role of the exploiter. To verify the performance of the presented algorithm, extensive experiments are executed on CEC2013 benchmark functions. Additionally, the efficiency of the proposed multi-group mechanism is analyzed in-depth. The experimental results reveal that the proposed multi-group mechanism improves FA and provides a suitable solution for most CEC2013 problems with different dimensions. Especially, its performance remains robust, where the problems become more complex.

In the past decade, rapid development in digital communication has led to prevalent use of digital images. More importantly, confidentiality issues have also come up recently due to the increase in digital image transmission across the Internet. Therefore, it is necessary to provide high imperceptibility and security to digitally transmitted images. In this paper, a novel blind digital image watermarking scheme is introduced tackling secured transmission of digital images, which provides a higher quality regarding both imperceptibility and robustness parameters. A block based hybrid IWT- SVD transform is implemented for robust transmission of digital images. To ensure high watermark security, the watermark is encrypted using a Pseudo random key which is generated adaptively from cover and watermark images. An encrypted watermark is embedded in randomly selected low entropy blocks to increase the security as well as imperceptibility. Embedding positions within the block are identified adaptively using a Blum–Blum–Shub Pseudo random generator. To ensure higher visual quality, Initial Scaling Factor (ISF) is chosen adaptively from a cover image using image range characteristics. ISF can be optimized using Nature Inspired Optimization (NIO) techniques for higher imperceptibility and robustness. Specifically, the ISF parameter is optimized by using three well-known and novel NIO-based algorithms such as Genetic Algorithms (GA), Artificial Bee Colony (ABC), and Firefly Optimization algorithm. Experiments were conducted for the proposed scheme in terms of imperceptibility, robustness, security, embedding rate, and computational time. Experimental results support higher effectiveness of the proposed scheme. Furthermore, performance comparison has been done with some of the existing state-of-the-art schemes which substantiates the improved performance of the proposed scheme.