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The term artificial intelligence (AI) was coined in the 1950s and it has successfully made its way into different fields of medicine. Forensic sciences and AI are increasingly intersecting fields that hold tremendous potential for solving complex criminal investigations. Considering the great evolution in the technologies applied to forensic genetics, this literature review aims to explore the existing body of research that investigates the application of AI in the field of forensic genetics. Scopus and Web of Science were searched: after an accurate evaluation, 12 articles were included in the present systematic review. The application of AI in the field of forensic genetics has predominantly focused on two aspects. Firstly, several studies have investigated the use of AI in haplogroup analysis to enhance and expedite the classification process of DNA samples. Secondly, other research groups have utilized AI to analyze short tandem repeat (STR) profiles, thereby minimizing the risk of misinterpretation. While AI has proven to be highly useful in forensic genetics, further improvements are needed before using these applications in real cases. The main challenge lies in the communication gap between forensic experts: as AI continues to advance, the collaboration between forensic sciences and AI presents immense potential for transforming investigative practices, enabling quicker and more precise case resolutions.

Progress in DNA profiling techniques has made it possible to detect even the minimum amount of DNA at a crime scene (i.e., a complete DNA profile can be produced using as little as 100 pg of DNA, equivalent to only 15–20 human cells), leading to new defense strategies. While the evidence of a DNA trace is seldom challenged in court by a defendant’s legal team, concerns are often raised about how the DNA was transferred to the location of the crime. This review aims to provide an up-to-date overview of the experimental work carried out focusing on indirect DNA transfer, analyzing each selected paper, the experimental method, the sampling technique, the extraction protocol, and the main results. Scopus and Web of Science databases were used as the search engines, including 49 papers. Based on the results of this review, one of the factors that influence secondary transfer is the amount of DNA shed by different individuals. Another factor is the type and duration of contact between individuals or objects (generally, more intimate or prolonged contact results in more DNA transfer). A third factor is the nature and quality of the DNA source. However, there are exceptions and variations depending on individual characteristics and environmental conditions. Considering that secondary transfer depends on multiple factors that interact with each other in unpredictable ways, it should be considered a complex and dynamic phenomenon that can affect forensic investigation in various ways, for example, placing a subject at a crime scene who has never been there. Correct methods and protocols are required to detect and prevent secondary transfer from compromising forensic evidence, as well as the correct interpretation through Bayesian networks. In this context, the definition of well-designed experimental studies combined with the use of new forensic techniques could improve our knowledge in this challenging field, reinforcing the value of DNA evidence in criminal trials.

In the present study, the metric properties of dynamic footprints were analysed using non-colouring method in relation with body parameters and compared with static footprint measurements. The results of the study provide a better understanding of the relationship between static and dynamic footprints, which may be useful for biological profiling that allows more accurate identification. Stature, body weight, five length and two width parameters of dynamic (walking) footprints of young Slovak adults (65 females and 68 males) were analysed. Pearson correlation coefficients were calculated and equations for prediction of stature and body weight by linear regression analysis and multiple regression analysis were developed. Intersex differences were confirmed for all parameters and bilateral for some. Statistically significant differences were found in all measurements (p-value>0.05), except for the width of the standing and walking footprint in the mixed group. A positive correlation was found between the selected footprint diameters with stature (max - r = 0.82) and body weight (max - r = 0.70). Stature could be calculated with an accuracy of up to 4.40 cm and body weight with an accuracy of up to 9.56 kg. The results of the present study show that selected measurements of dynamic footprints correlate with stature and body weight. These results could be used in biological profiling in the medical and forensic fields. •Body weight prediction equations from walking footprints were developed.•Stature prediction equations from dynamic footprints were calculated.•Higher correlations of dynamic footprints parameters with stature were observed.•Significant differences between length measurements of standing and walking footprints were confirmed.•Non-coloring cream method is reliable to collect footprints.

DNA transfer, whether intentional or not, is becoming an important part of forensic evidence gathering and analysis. This study seeks to determine the impact of liquid antibacterial soap and hand sanitizer use on the DNA present in the palms of hands that may potentially reduce direct transfer. Twelve volunteers were enrolled and typed. Afterwards, each palm was swabbed without considering previous activities to collect background DNA profiles. Subsequently, each subject washed his/her hands for 30 s with antibacterial soap first, and after with sanitizing gel and then air-dried them; after drying, each palm was immediately swabbed for DNA typing. The results of this study demonstrated that the possibility of recovering a complete profile from a hand swab is strictly related to previous activity: considering the results of the samples taken before handwashing, there is the possibility of having a median recovery of 80.01%, while it is very low (median recovery was 3.43%) after hand washing with antibacterial soap followed by alcohol-based hand sanitizer. Analyzing the results after handwashing, we were unable to detect any drop-in alleles. Moreover, we determined that in 11/12 samples, less than nine alleles were detected: considering that the kit used for the profiling could analyze 23 short tandem repeats (STRs), it is possible to conclude that we obtained inconclusive profiles. Based on the findings of the present study, it is more difficult to obtain a perpetrator’s profile if he/she used liquid antibacterial soap and hand sanitizer immediately before the criminal action.

Since December 2019, the global outbreak of coronavirus disease had a significant impact on humanity. Because of the large number of casualties worldwide, the WHO (World Health Organization) declared the coronavirus disease caused by SARS-CoV-2 a pandemic. Since the start of the pandemic, facial masks have become essential as well as mandatory to protect ourselves from COVID-19. As a result of the pandemic, healthcare professionals (HCPs) have been required to wear personal protective equipment (PPE) for extended periods. Wearing face masks for an extended period has been shown to have several negative effects on HCPs. Additionally, face masks have hampered the use of digital techniques for facial identification. This paper examines the effects of wearing face masks for an extended period, as well as the effect of wearing face masks on facial identification technology. The Web of Science, PubMed, and Scopus databases were searched and screened for relevant studies. According to the current review, prolonged use of masks was found to be associated with adverse effects on the face and skin, including acne, redness, rashes, and itching. The use of masks also resulted in headaches, hypoxic conditions, and changes in voice and speech parameters. This communication in no way intends to advocate the discontinuation of wearing masks, on the contrary, the primary goal of this article is to spread awareness about the adverse effects associated with prolonged use of facial masks (N95, KF94, or surgical). This will help in increasing compliance with mask mandates by helping to develop preventive solutions to the problems that tend to deter the general public. This also demonstrates how the use of masks has become a challenge for facial recognition technologies.

Third molar extraction is the most common procedure in oral and maxillofacial surgery. Third molars are considered less functional than other teeth and are often extracted. Sometimes, they are also used for auto-transplantation for the benefit of oral rehabilitation. Since many biological factors are involved in this surgical approach, herein, we outline a review of the biological characteristics of medico-legal/forensic interest, in addition to presenting a successful clinical case. A scoping review of currently available research data (following the principles of PRISMA-ScR or the Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews) on third molar auto-transplantation was conducted by drawing upon the main databases (Scopus, PubMed, Google Scholar and LILACS) to evaluate biological and clinical characteristics possibly relatable to forensic issues. All the collected data were summarized and elaborated on for the purpose of this article. A patient underwent extraction of the right upper first molar and auto-transplantation of the unerupted ipsilateral third molar. Many biologic and clinical factors are involved in the success of this clinical procedure. Knowledge of third molar anatomy, of its development and viable surgical approaches are all essential elements; just as important are the treatment of the tooth before and after transplantation and the integrity of the periodontal ligament. Follow-up of the clinical case for 5 years made it possible to verify the stability of the procedure over time. Third molar auto-transplantation is feasible and cost-effective. However, the use of third molars as donor teeth in auto-transplantation may have medico-legal implications. The lack of official protocols and consistent evidence-based guidelines for operators still prevent such a procedure from becoming mainstream; therefore, it is viewed with suspicion by clinicians and patients, even though the biological factors herein detected point to a reasonably high degree of safety. The understanding of many specific biological and clinical factors involved in the stability of third molar auto-transplantation allows for a thorough understanding of the forensic implications relevant to clinical practice. Effective communication and information provision are therefore of utmost importance, in the interest of both patients and doctors.

Ten years after the adoption of the fifth edition of the Diagnostic and Statistical Manual of Mental Disorders (DSM-5), and with the recent publication of a text revision (DSM-5-TR), the limitations of this system for diagnostic classification of mental disorders remain. However, given that the alternatives that have been proposed have their own serious problems, DSM is likely to be retained for some time to come. It is generally accepted by courts as reflecting expert opinion. This paper highlights problems with the reliability and validity of DSM diagnoses, the tendency to overdiagnosis of certain categories, leading to problems with the use of these criteria in the courts, both in criminal and civil laws. The review argues that the categories in the DSM should be considered heuristics, not as disease entities equivalent to medical diagnoses supported by biomarkers and endophenotypes.

Edged weapons (sometimes referred to as sharp weapons or blades) are an increasing threat to military personnel, the blue light community (police, ambulance, firefighters, other first responders) and the general public worldwide. The use of edged weapons in criminal and terrorist incidents internationally means the forensic community needs an awareness of the technology of edged weapons, how they are used, the damage (clothing and wounding) that might be caused and any other forensic implications. In this paper, the magnitude of the problem is presented, prior research summarised and implications for forensic investigations discussed.

Although adjustment disorder (AD) is considered as residual diagnosis and receives little attention in research, it plays an important role in clinical practice and also assumes an increasingly important role in the field of legal medicine, where the majority of diagnostic frameworks (eg, mobbing) often refer to AD. Our study aimed to look for specific stressor differences among demographic and clinical variables in a naturalistic setting of patients with AD. A restrospective statistical analysis of the data of patients diagnosed with AD from November 2009 to September 2012, identified via manual search from the archive of the outpatient setting at the University Unit of Psychiatry \"A. Fiorini\" Hospital, Terracina (Latina, Italy), was performed. The sample consisted of 93 patients (46 males and 47 females), aged between 26 and 85, with medium-high educational level who were mainly employed. In most cases (54.80%), a diagnosis of AD with mixed anxiety and depressed mood was made. In all, 72% of the sample reported a negative family history for psychiatric disorders. In 22.60%, a previous history of psychopathology, especially mood disorders (76.19%), was reported. The main stressors linked to the development of AD were represented by working problems (32.30%), family problems (23.70%), and/or somatic disease (22.60%) with significant differences with respect to age and sex. Half of the patients were subjected to a single first examination; 24.47% requested a copy of medical records. Confirming previous data from previous reports, our results suggest that AD may have a distinct profile in demographic and clinical terms. Increased scientific attention is hoped, particularly focused on addressing a better definition of diagnostic criteria, whose correctness and accuracy are critical, especially in situations with medicolegal implications.