Explore the vast range of titles available.

Pondweed is a rhizomatous perennial weed of aquatic habitats that recently adapted to rice ecosystems in northern Iran. Two field experiments were conducted at the Rice Research Institute of Iran to determine the impact of pondweed on rice yield and identify effective herbicides for pondweed control. The focus of the first study was to evaluate the herbicides commonly used in Iranian rice, including butachlor, pretilachlor, oxadiargyl, pendimethalin, thiobencarb, and bensulfuron-methyl. None of these herbicides effectively controlled pondweed, except bensulfuron, which reduced pondweed biomass by ≥95% and produced 26% higher rough rice grain yield than the nontreated plots. The second experiment evaluated the performance of acetolactate synthase–inhibiting herbicides on pondweed control, rough rice yield, and pondweed regrowth. Herbicide efficacy on pondweed varied from 36% to 100%. Five preemergence herbicides, bensulfuron at 45 g ai ha–1, flucetosulfuron at 30 g ai ha–1, triafamone plus ethoxysulfuron at 40 g ai ha–1, and metsulfuron-methyl at 15 g ai ha–1, provided ≥98% control of pondweed. Use of postemergence herbicides penoxsulam at 35 g ai ha–1, bispyribac-sodium at 30 g ai ha–1, and pyribenzoxim at 35 g ai ha–1 provided 36%, 89%, and 93% pondweed control, respectively. Rough rice yields ranged from 107% to 124% in herbicide-treated plots compared with the nontreated plots. Soil-applied herbicide treatments produced higher (≥119%) yield than the hand-weeded control or foliar-applied herbicides. Pondweed regrowth was affected by herbicides and was variable. Soil-applied residual herbicides metazosulfuron, flucetosulfuron, and metsulfuron provided complete control of pondweed and prevented regrowth. In contrast, pondweed regrowth in other soil- and foliar-applied herbicide treatments occurred, indicating their lesser translocation to underground vegetative rhizomes. This study shows that although most sulfonylurea herbicides can control pondweed effectively to achieve high rough rice yield, only a few soil-applied herbicides were able to prevent pondweed regrowth. Nomenclature: Bensulfuron-methyl; bispyribac-sodium; butachlor; flucetosulfuron; metazosulfuron; metsulfuron-methyl; oxadiargyl; pendimethalin; penoxsulam; pretilachlor; pyribenzoxim; thiobencarb; triafamone plus ethoxysulfuron; pondweed, Potamogeton nodosus Poir. ‘PTMNO’; rice, Oryza sativa L.

Grass pollen triggers nearly 30% of bronchial allergic asthma cases. While most Q8 research focuses on pollen allergens, pollen lipids may also influence allergic reactions. Previous studies demonstrated that Timothy grass (TG, Phleum pratense) lipids, such as phytoprostanes, can activate immune cells, promoting pro-allergic responses. However, the role of water-insoluble pollen glycolipids in allergic airway inflammation remains unclear. Thus, this study aimed to isolate and characterize glycolipids from TG pollen and evaluate their bioactivity in allergic airway inflammation. Lipids were extracted from the water-insoluble pollen fraction, separated by silica gel, and fractionated by HPLC. GC-MS, HR ESI-MS, and NMR confirmed the presence of di-galactosyldiacylglycerol (DGDG). The biological activity of fractions containing DGDG (DGDG-3 and DGDG-4) and synthetic DGDG variants was tested in vitro in murine and human cell systems and in vivo in mice. Fraction 4 induced strong proliferation of murine NKT cells and upregulated CD69 expression in human NKT cells. Synthetic DGDG variants (DGDG-1, DGDG-2, and DGDG-3) with defined acylation profiles stimulated robust NKT-cell proliferation, with DGDG-2 and DGDG-3 increasing IL-13 production, one of the key Th2 cytokines. In vivo, only these variants caused lung inflammation marked by eosinophil infiltration but did not increase airway resistance. This study reveals for the first time the structure-dependent role of DGDG of TG pollen grains in immune cell recognition in the context of allergic inflammation. Our data may pave the way for therapies targeting lipid components in combination with protein allergens.

Grass carp (Ctenopharyngodon idella) is one of the most economically important fish in China, and its production is commonly lost due to GCRV infection. To understand the molecular mechanism of GCRV resistance in grass carp, we compared the spleen transcriptome of the GCRV-resistant and susceptible individuals under GCRV infection (Res-Sus) and the GCRV-resistant individuals under different conditions of injection with GCRV and PBS (Res-Ctl). A total of 87.56 GB of clean data were obtained from 12 transcriptomic libraries of spleen tissues. A total of 379 DEGs (156 upregulated genes and 223 downregulated genes) were identified in the comparison group Res-Ctl. A total of 1207 DEGs (633 upregulated genes and 574 downregulated genes) were identified in the comparison group Res-Sus. And 54 DEGs were shared including immune-related genes of stc2 (stanniocalcin 2), plxna1 (plexin A1), ifnα (interferon alpha), cxcl 11 (C-X-C motif chemokine ligand 11), ngfr (nerve growth factor receptor), mx (MX dynamin-like GTPase), crim1 (cysteine-rich transmembrane BMP regulator 1), plxnb2 (plexin B2), and slit2 (slit guidance ligand 2). KEGG pathway analysis revealed significant differences in the expression of genes mainly involved in immune system and signal transduction, including antigen processing and presentation, Toll-like receptor signaling pathway, natural killer cell-mediated cytotoxicity, and Hippo signaling pathway. This study investigates the immune mechanism of the resistance to GCRV infection in grass carp and provides useful information for the development of methods to control the spread of the GCRV infection.

Some species of the genus Aegilops , a wild relative of wheat, carry chromosomes that after introducing to wheat exhibit preferential transmission to progeny. Their selective retention is a result of the abortion of gametes lacking them due to induced chromosomal aberrations. These chromosomes are termed Gametocidal ( Gc ) and, based on their effects, they are categorized into three types: mild, intense or severe, and very strong. Gc elements within the same homoeologous chromosome groups of Aegilops (II, III, or IV) demonstrate similar Gc action. This review explores the intriguing dynamics of Gc chromosomes and encompasses comprehensive insights into their source species, behavioral aspects, mode of action, interactions, suppressions, and practical applications of the Gc system in wheat breeding. By delving into these areas, this work aims to contribute to the development of novel plant genetic resources for wheat breeding. The insights provided herein shed light on the utilization of Gc chromosomes to produce chromosomal rearrangements in wheat and its wild relatives, thereby facilitating the generation of chromosome deletions, translocations, and telosomic lines. The Gc approach has significantly advanced various aspects of wheat genetics, including the introgression of novel genes and alleles, molecular markers and gene mapping, and the exploration of homoeologous relationships within Triticeae species. The mystery lies in why gametes possessing Gc genes maintain their normality while those lacking Gc genes suffer abnormalities, highlighting an unresolved research gap necessitating deeper investigation.

Previous studies revealed that Kanglaite (KLT) exhibits antitumor and immunomodulatory activities. In the present study, we show that KLT treatment stimulated the immune response by increasing the number of T cells and natural killer (NK) cells in the blood of hepatocellular carcinoma (HCC) patients. Experiments in tumor-bearing mice were further designed in order to explore the effects of KLT on the immune system and the underlying molecular mechanisms. The results showed that KLT improves the tumor cell transplantation-induced reduction in the serum level of the cytokines IFN-γ and IL-2, and rescues the levels of CD4+ T cells in host mice. These events enhanced the cytotoxic activities of natural killer and CD8+ T cells against the hepatic HepG2 cancer cells. KLT administration further increased the mRNA level of certain nuclear factor κB (NF-κB)-responsive genes in CD4+ cells. The chromatin immunoprecipitation assay showed that KLT increases the association of the NF-κB p65 subunit to the promoter regions of interleukin (IL)-2- and B-cell lymphoma (Bcl)-2-encoding genes in CD4+ T cells. Our study demonstrated that KLT is the main active ingredient of coix seed exhibiting anticancer and immunomodulatory properties. Induction of NF-κB-mediated gene transcription in CD4+ T cells is involved in the immunomodulatory activity of KLT.

Epithelial cell adhesion molecule (EpCAM) is a Ca 2+ -independent and relatively weak adhesion molecule, which has been extensively investigated in mammalian models. However, the functional roles of its fish homolog are largely unknown. In the present study, we explored the biological properties of grass carp EpCAM (gcEpCAM) in a fish kidney cell line (CIK) via overexpression of gcEpCAM or gcEpCAM intracellular domain (gcEpICD) deletion mutant. Results showed that gcEpCAM overexpression significantly changed the cell morphology, and the proliferation of the cells transfected with gcEpCAM was significantly decreased when compared to the control cells, which is unexpectedly opposite to the increasing effects induced by its mammalian homolog. Moreover, overexpression of gcEpICD deletion mutant had no effect on cell proliferation, indicating gcEpICD’s involvement in the cell growth control that is concerted with its role in mammalian model. Additionally, gcEpCAM overexpression increased cell migration which is at least partially consistent with the findings in mammalian cells in which EpCAM expression both positively and negatively affects cell migration. It is worth noting that gcEpICD was not essential to the stimulatory effect of gcEpCAM on cell migration, but overexpression of human EpICD in tumor cells increases cell migration, suggesting the functional discrepancy of EpICD in fish and mammals. In conclusion, we elucidated the cellular functionality of EpCAM in fish cells which will be of benefit to defining the functions of fish EpCAM and also provide rewarding information on the functional evolution of EpCAM in vertebrates.

Ska act Reel Big Fish is teaming up with punk act Anti-Flag for a winter tour that comes through the Rock Hall City for a show Jan. 10 at the House of Blues. Greensky Bluegrass has carved out its own niche playing bluegrass music with a rock 'n' roll edge.

  Ska act Reel Big Fish is teaming up with punk act Anti-Flag for a winter tour that comes through the Rock Hall City for a show Jan. 10 at the House of Blues. Greensky Bluegrass has carved out its own niche playing bluegrass music with a rock 'n' roll edge.

Ska act Reel Big Fish is teaming up with punk act Anti-Flag for a winter tour that comes through the Rock Hall City for a show Jan. 10 at the House of Blues. Greensky Bluegrass has carved out its own niche playing bluegrass music with a rock 'n' roll edge.