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Hypersaline environments with salt concentrations up to NaCl saturation are inhabited by a great diversity of microorganisms belonging to the three domains of life. They all must cope with the low water activity of their environment, but different strategies exist to provide osmotic balance of the cells' cytoplasm with the salinity of the medium. One option used by many halophilic Archaea and a few representatives of the Bacteria is to accumulate salts, mainly KCl and to adapt the entire intracellular machinery to function in the presence of molar concentrations of salts. A more widespread option is the synthesis or accumulation of organic osmotic, so-called compatible solutes. Here, we review the mechanisms of osmotic adaptation in a number of model organisms, including the KCl accumulating Halobacterium salinarum (Archaea) and Salinibacter ruber (Bacteria), Halomonas elongata as a representative of the Bacteria that synthesize organic osmotic solutes, eukaryotic microorganisms including the unicellular green alga Dunaliella salina and the black yeasts Hortaea werneckii and the basidiomycetous Wallemia ichthyophaga, which use glycerol and other compatible solutes. The strategies used by these model organisms and by additional halophilic microorganisms presented are then compared to obtain an integrative picture of the adaptations to life at high salt concentrations in the microbial world.

Extreme marine environments cover more than 50% of the Earth’s surface and offer many opportunities for investigating the biological responses and adaptations of organisms to stressful life conditions. Extreme marine environments are sometimes associated with ephemeral and unstable ecosystems, but can host abundant, often endemic and well-adapted meiofaunal species. In this review, we present an integrated view of the biodiversity, ecology and physiological responses of marine meiofauna inhabiting several extreme marine environments (mangroves, submarine caves, Polar ecosystems, hypersaline areas, hypoxic/anoxic environments, hydrothermal vents, cold seeps, carcasses/sunken woods, deep-sea canyons, deep hypersaline anoxic basins [DHABs] and hadal zones). Foraminiferans, nematodes and copepods are abundant in almost all of these habitats and are dominant in deep-sea ecosystems. The presence and dominance of some other taxa that are normally less common may be typical of certain extreme conditions. Kinorhynchs are particularly well adapted to cold seeps and other environments that experience drastic changes in salinity, rotifers are well represented in polar ecosystems and loriciferans seem to be the only metazoan able to survive multiple stressors in DHABs. As well as natural processes, human activities may generate stressful conditions, including deoxygenation, acidification and rises in temperature. The behaviour and physiology of different meiofaunal taxa, such as some foraminiferans, nematode and copepod species, can provide vital information on how organisms may respond to these challenges and can provide a warning signal of anthropogenic impacts. From an evolutionary perspective, the discovery of new meiofauna taxa from extreme environments very often sheds light on phylogenetic relationships, while understanding how meiofaunal organisms are able to survive or even flourish in these conditions can explain evolutionary pathways. Finally, there are multiple potential economic benefits to be gained from ecological, biological, physiological and evolutionary studies of meiofauna in extreme environments. Despite all the advantages offered by meiofauna studies from extreme environments, there is still an urgent need to foster meiofauna research in terms of composition, ecology, biology and physiology focusing on extreme environments.

This study explores the use of a native hypersaline microalgae strain from Oklahoma, Komvophoron sp., for dual purposes: treating Flowback (FW) and Produced Water (PW), and generating algal biomass. The wastewaters were analyzed before and after treatment, and the resulting biomass was characterized for moisture, volatile matter, fixed carbon, and ash content. Komvophoron sp. thrived in both FW and PW, achieving higher biomass concentrations when cultivated in PWs compared to FWs despite nutrient limitations. It also showed high specific growth rates in both water types. The biomass had an energy content of 16–17 MJ kg⁻¹, suitable for biofuel feedstock, although salinity slightly reduced this value. Algal cultivation fully removed ammonia and significantly reduced nitrate, phosphate, boron, and metals such as zinc, manganese, and iron. This approach shows strong potential for reducing the environmental impact of hydraulic fracturing while producing biomass for biofuels and other industrial uses.

Hypersaline wastewater is difficult to treat due to the inhibition of salt stress on microbes’ viability and metabolic capabilities. Haloarchaea, native microorganisms that thrive in hypersaline habitats, overcome this key obstacle naturally. This review provides a comprehensive overview of the metabolic versatility of Haloarchaea in hypersaline wastewater treatment, including carbon, nitrogen, phosphorus, sulfur, and heavy metal metabolism. It also analyzes factors affecting pollutant removal and addresses metabolic mechanisms. Additionally, haloarchaea microbial characteristics and strategies to cope with salt stress are highlighted. Finally, the biotechnological potential of biomolecules produced from haloarchaea is investigated. To get better insight into the potential of haloarchaea, a deeper investigation of basic metabolism and more in-depth studies of their genomics and applications in actual wastewater are also necessary. Haloarchaea are excellent candidates to treat hypersaline wastewater because they show an extensive metabolic versatility of carbon, nitrogen, phosphorus, sulfur, and heavy metal.The ‘salt-in’ strategy, mainly the accumulation of K+ and expulsion of Na+, is used by haloarchaea to cope with salt stress.Haloarchaea have special features and biomolecules, such as haloarchaeal enzymes, gas vesicles, and poly-β-hydroxyalkanoates, that make them worth exploring for their biotechnological and industrial potential.The discovery of polyextremophilic (halophilic, thermophilic, and alkaliphilic) haloarchaea promotes their real-world applications in wastewater treatment under multiple stresses.

NaCl-saturated brines such as saltern crystalliser ponds, inland salt lakes, deep-sea brines and liquids-of-deliquescence on halite are commonly regarded as a paradigm for the limit of life on Earth. There are, however, other habitats that are thermodynamically more extreme. Typically, NaCl-saturated environments contain all domains of life and perform complete biogeochemical cycling. Despite their reduced water activity, ∼0.755 at 5 M NaCl, some halophiles belonging to the Archaea and Bacteria exhibit optimum growth/metabolism in these brines. Furthermore, the recognised water-activity limit for microbial function, ∼0.585 for some strains of fungi, lies far below 0.755. Other biophysical constraints on the microbial biosphere (temperatures of >121°C; pH > 12; and high chaotropicity; e.g. ethanol at >18.9% w/v (24% v/v) and MgCl2 at >3.03 M) can prevent any cellular metabolism or ecosystem function. By contrast, NaCl-saturated environments contain biomass-dense, metabolically diverse, highly active and complex microbial ecosystems; and this underscores their moderate character. Here, we survey the evidence that NaCl-saturated brines are biologically permissive, fertile habitats that are thermodynamically mid-range rather than extreme. Indeed, were NaCl sufficiently soluble, some halophiles might grow at concentrations of up to 8 M. It may be that the finite solubility of NaCl has stabilised the genetic composition of halophile populations and limited the action of natural selection in driving halophile evolution towards greater xerophilicity. Further implications are considered for the origin(s) of life and other aspects of astrobiology.

NASA’s current mandate is to land humans on Mars by 2033. Here, we demonstrate an approach to produce ultrapure H₂ and O₂ from liquid-phase Martian regolithic brine at ∼−36 °C. Utilizing a Pb₂Ru₂O7–δ pyrochlore O₂-evolution electrocatalyst and a Pt/C H₂-evolution electrocatalyst, we demonstrate a brine electrolyzer with >25× the O₂ production rate of the Mars Oxygen In Situ Resource Utilization Experiment (MOXIE) from NASA’s Mars 2020 mission for the same input power under Martian terrestrial conditions. Given the Phoenix lander’s observation of an active water cycle on Mars and the extensive presence of perchlorate salts that depress water’s freezing point to ∼−60 °C, our approach provides a unique pathway to life-support and fuel production for future human missions to Mars.

It has become clear that estuaries with low rates of freshwater inflow are an important but overlooked sphere of estuarine science. Low-inflow estuaries (LIEs) represent a major class of estuary long downplayed because observations do not fit well in the dominant estuary paradigm, which was developed in perennially wet climates. Rather than being rare and unusual, it is now evident that LIEs are common globally and an alternate estuary paradigm within the idea of an estuary as the place where a river meets the sea. They are found mostly in areas with arid, semi-arid, or seasonally arid climates, but LIE phenomena are also found in estuaries along mountainous coasts with small watersheds and short-tailed hydrographs. Inflows can be defined as “low” relative to basin volume, tidal mixing, evaporative losses, or wave forcing at the mouth. The focus here is on common physical phenomena that emerge in low-inflow estuaries—how low river flow is expressed in estuaries. The most common is hypersalinity (and the associated potential for inverse conditions), which develops where there is a net negative water balance. However, in small microtidal estuaries, low inflow results in mouth closure even as a positive water balance may persist, accounting for extreme stratification. Attention is also given to the longitudinal density gradient and the occurrence of thermal estuaries and inverse estuaries. Finally, ocean-driven estuaries are highlighted where marine subsidies (nutrients, particulates) dominate watershed subsidies. While climate change is altering freshwater inflow to estuaries, locally driven changes are generally more important and this presents an opportunity to restore estuaries through restoring estuarine hydrology.

Produced water (PW), the largest waste stream generated in oil and gas industries, has the potential to be a harmless product rather than being a waste. Biological processes using microorganisms have proven useful to remediate PW contaminated by petroleum hydrocarbons, complex organic chemicals, and solvents. In particular, the bioremediation of PW using algae is an eco-friendly and low-cost approach due to algae’s ability to utilize certain pollutants as nutrient sources. Therefore, the utilization of PW as an algal growth medium has a great potential to eliminate chemicals from the PW and minimize the large volumes of freshwater needed for cultivation. Although several reviews describing the bioremediation of PW have been published, to the best of our knowledge, no review has exclusively focused on the algae-based PW treatment. Therefore, the present review is dedicated to filling this gap by portraying the many different facets of the algae cultivation in PW. Several algal species that are known to thrive in a wide range of salinity and the critical steps for their cultivation in hypersaline PW have been identified. Overall, this comprehensive review highlights the PW bioremediation using algae and brings attention to utilizing PW to grow biomass that can be processed to generate biofuels and useful bioproducts.

Abstract Sediments underlying the solar salterns of S’Avall are anoxic hypersaline ecosystems dominated by anaerobic prokaryotes, and with the especial relevance of putative methanogenic archaea. Slurries from salt-saturated sediments, diluted in a gradient of salinity and incubated for > 4 years revealed that salt concentration was the major selection force that deterministically structured microbial communities. The dominant archaea in the original communities showed a decrease in alpha diversity with dilution accompanied by the increase of bacterial alpha diversity, being highest at 5% salts. Correspondingly, methanogens decreased and in turn sulfate reducers increased with decreasing salt concentrations. Methanogens especially dominated at 25%. Different concentrations of litter of Posidonia oceanica seagrass added as a carbon substrate, did not promote any clear relevant effect. However, the addition of ampicillin as selection pressure exerted important effects on the assemblage probably due to the removal of competitors or enhancers. The amended antibiotic enhanced methanogenesis in the concentrations ≤ 15% of salts, whereas it was depleted at salinities ≥ 20% revealing key roles of ampicillin-sensitive bacteria. Microbial ecology of hyper- and saline methanogenic sediments from solar salterns of S'Avall (Balearic Islands, Spain).

Extremotolerant and extremophilic fungi are an important part of microbial communities that thrive in extreme environments. Among them, the black yeasts are particularly adaptable. They use their melanized cell walls and versatile morphology, as well as a complex set of molecular adaptations, to survive in conditions that are lethal to most other species. In contrast to extremophilic bacteria and archaea, these fungi are typically extremotolerant rather than extremophilic and exhibit an unusually wide ecological amplitude. Some extremely halotolerant black yeasts can grow in near-saturated NaCl solutions, but can also grow on normal mycological media. They adapt to the low water activity caused by high salt concentrations by sensing their environment, balancing osmotic pressure by accumulating compatible solutes, removing toxic salt ions from the cell using membrane transporters, altering membrane composition and remodelling the highly melanized cell wall. As protection against extreme conditions, halotolerant black yeasts also develop different morphologies, from yeast-like to meristematic. Genomic studies of black yeasts have revealed a variety of reproductive strategies, from clonality to intense recombination and the formation of stable hybrids. Although a comprehensive understanding of the ecological role and molecular adaptations of halotolerant black yeasts remains elusive and the application of many experimental methods is challenging due to their slow growth and recalcitrant cell walls, much progress has been made in deciphering their halotolerance. Advances in molecular tools and genomics are once again accelerating the research of black yeasts, promising further insights into their survival strategies and the molecular basis of their adaptations. Key points • Black yeasts show remarkable adaptability to environmental stress • Black yeasts are part of microbial communities in hypersaline environments • Halotolerant black yeasts utilise various molecular and morphological adaptations