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The present work is devoted to the investigation of the effect of pulsed ohmic heating (POH) on cells membrane damage and intensification of polyphenols extraction from red grape pomace. Untreated, POH-treated and freeze-thawed samples were compared. The effects of electric field strength ( E  = 100–800 V/cm) and the percentage of ethanol in water ( E / W  = 0–50 %) on polyphenols extraction were discussed. Measurements of electrical conductivity and electric energy consumption were performed for POH pretreatment optimization. Results show that POH treatment results in cells membrane denaturation. This permeabilization increases with the elevation of electric field strength and temperature. POH pretreatment accelerates the extraction kinetics of total polyphenols from grape pomace. Freeze-thawed samples are always accompanied with a high degree of cell damage and high concentration of polyphenols in the extract. The highest extraction yields were obtained with a POH pretreatment at 400 V/cm followed by a diffusion step for 60 min at 50 °C and with a solvent composed of 30 % of ethanol in water. In these conditions, the polyphenol content was 36 % more than untreated samples. The proposed technique (POH pretreatment) appears to be promising for future industrial applications of polyphenols extraction from pomace.

A lab-scale continuous ohmic heating (COH) system was developed, and its performance was studied for pineapple juice heating as a model sample. The effect of independent parameters [°Brix/Acid (unstandardized, 18, 22, 26) and flow rate (80–120 mL/min) of juice and electric field strength (EFS: 25–45 V/cm)] were analysed for responses viz. come-up-time, heating rate (HR) and system performance coefficient (SPC). The full factorial experimental design was used for this study. The results showed that with an increase in °Brix/Acid, the % acidity and electrical conductivity decreased significantly (p < 0.05); thus, the come-up-time to reach 90 °C increased significantly. The HR was significantly (p < 0.05) influenced by °Brix/Acid and EFS but less so by flow rates at higher EFS. The SPC was more than 0.90 and reduced significantly (p < 0.05) with an increase in °Brix/Acid and flow rate. The HR was modeled using a feed-forward back-propagation artificial neural network (ANN) with the best topology of 3, 5, and 1 neurons in the input (independent), hidden, and output (response) layers, respectively. The model performed efficiently, which is evident from the high R2 (0.998) and low RMSE (1.255). Thus, the COH, with its high efficiency and HR, can effectively be used to process fruit juice.

Pineapple core is considered a processing by-product. This study proposed and evaluated an ohmic heating extraction-based valorization platform to obtain value-added bioactive compounds from pineapple core and studied the effects of four important processing parameters. In this sense, a Taguchi design (L16(4)4) was used to assess the effects of temperature (70, 80, 90, and 100 °C), time (15, 30, 45, and 60 min), voltage (110, 160, 210, and 260 V), and frequency (60, 340, 620, and 900 Hz) on heating rate, come-up time, energy consumption, system performance efficiency, total phenolic compounds (TPC), DPPH, and ABTS. Finally, a side-by-side comparison of optimized ohmic heating (OOH) and conventional extraction was performed, and chemical composition was compared by ultra-performance liquid chromatography equipped with photodiode array detection-mass (UPLC-DAD-ESI-MS-MS). According to the results, increasing temperatures enhanced system performance efficiency but negatively affected TPC and antioxidant values above 90 ℃. Similarly, prolonging the extraction (>30 min) decreased TPC. Further, increasing voltage (from 110 to 260 V) shortened the come-up time (from 35.75 to 5.16 min) and increased the heating rate (from 2.71 to 18.80 °C/min−1). The optimal conditions were 30 min of extraction at 80 °C, 160 V, and 900 Hz. Verification of the optimal conditions revealed that OOH yielded an extract with valuable bioactive compounds and saved 50% of the time and 80% of energy compared to the conventional treatment. The UPLC-DAD-ESI-MS-MS showed that there were similarities between the chemical profiles of the extracts obtained by conventional and OOH methods, while the concentration of major compounds varied depending on the extraction method. This information can help achieve sustainable development goals (SDGs) by maximizing the yield and minimizing energy and time consumption.

Phenolic compounds are important bioactive compounds identified in prickly pear peel that have important antioxidant and antimicrobial properties. However, conventional thermal extraction methods may reduce their bioactivity, and technologies such as high pressure (HP) and ohmic heating (OH) may help preserve them. In this study, both technologies were analyzed, individually and combined (250/500 MPa; 40/70 °C; ethanol concentration 30/70%), and compared with Soxhlet with regard to total phenolics, flavonoids, and carotenoids as well as antioxidant (ABTS, DPPH, ORAC), DNA pro-oxidant, and antimicrobial (inhibition halos, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), growth curves, and viable cells) activities of prickly pear peel extracts. Total phenolics extracted by each technology increased 103% (OH) and 98% (HP) with regard to Soxhlet, but the contents of total flavonoids and carotenoids were similar. Antioxidant activity increased with HP and OH (between 35% and 63%), and OH (70 °C) did not induce DNA degradation. The phenolic compound present in higher amounts was piscidic acid, followed by eucomic acid and citrate. In general, their extraction was significantly favored by HP and OH. Antimicrobial activity against 7 types of bacteria showed effective results only against S. aureus, S. enteritidis, and B. cereus. No synergetic or additive effect was observed for HP/OH.

The effect of recycling ohmic heating on the deacetylation, structure, and properties of chitosan prepared from Portunus trituberculatus crab shells was investigated. The effect of six proteases on deproteinization was examined. Deacetylation was carried out using four cycles of heating, in which heating methods included cyclic water bath heating, cyclic ohmic heating, and a combination of recycling ohmic and water bath heating. The structure and properties of chitosan were determined by Fourier transform infrared spectroscopy (FTIR), differential scanning calorimeter (DSC), scanning electron microscope (SEM), dielectric properties, and rheological properties. A combination of many factors, including acid protease and pepsin, was chosen for complex proteolytic deproteinization. Ohmic heating took 2/5 of the time and used 1/2000 of the energy required for water bath heating. FTIR, DSC, dielectric properties, and SEM results showed that the chitosan characteristic after ohmic heating treatment met commercial requirements. Chitosan’s FTIR spectra and dielectric loss values at low frequencies after three cycles of combined ohmic and water bath heating were most similar to those of commercial chitosan without destroying its thermal stability. After the three cycles, the degree of chitosan deacetylation using combined ohmic and water bath heating reached 94.38 ± 0.21%, improving deacetylation compared with the traditional method.

Ohmic heating is a promising alternative method to conventional heating for microbial inactivation. This study aimed to inactivate Listeria monocytogenes and assess some quality parameters in ready-to-consume liquid infant milk treated with different voltage gradients of ohmic heating. Different ohmic heating voltage gradients (5, 10, and 20V cm-1) were applied to the samples inoculated with Listeria monocytogenes (ATCC 13932) for 5 minutes. The application of 20 V cm-1 ohmic heating induced inactivation of Listeria monocytogenes at 4 minutes and resulted in approximately 5.34 log reduction; however, there was no significant reduction for the 5 and 10V cm-1 groups. Moreover, 20V cm-1 ohmic heating application did not cause any changes in pH, L*, and b* values. A significant decrease in a* value and an increase in hydroxymethylfurfural value was noted in this group.  In conclusion, the effectiveness of ohmic heating in pathogen inactivation depends on the applied electric field intensity and the application time. As a result, the ohmic heating conditions must be carefully determined for the infant milk to inactivate pathogens and ensure public health protection. The results of this study contain significant and beneficial data that can be disposed of the listeriosis risk associated with the consumption of infant milk in infants.

The present study investigates the application of ohmicsonication (OS) as a new hurdle technology for pasteurization of Not-from-concentrate orange juice (NFCOJ). OS process parameters to inactivate pectinmethylesterase (PME) activity in NFCOJ were optimized using response surface methodology. The influence of Sonication (S), Thermosonication (TS), Ohmic heating (OH) and OS on inactivation of PME were compared to conventional heat (CH) treatment. Their effects on physical, chemical and microbiological contents were included. In comparison to fresh orange juice, the inactivation of PME was 96%, 95%, 89%, 90% and 29% for OS, OH, TS, CH and S treatments, respectively. Highest cloud value was obtained for OS (1.240 A) treatment. OS treatment gave a lower vitamin C loss compared to TS, OH and CH treatments. A significant increase in the total phenolic content were obtained in the following order OS > TS > OH > CH. OS treated juice also contained the lowest value of hydroxymethyl furfural (0.90 mg/L) compared to OH (0.95 mg/L), TS (1.37 mg/L) and CH (2.72 mg/L) treated samples. Overall, the results indicated that OS can be integrated as a substitute to pasteurization of NFCOJ.

The effect of blanching by ohmic heating (OH) on the kinetics of osmotic dehydration of strawberries was studied. Ohmic heating parameters obtained at two temperatures (65 and 85 °C). The osmotic dehydration (OD) parameters are the temperature (26 and 37 °C) and the sucrose in osmotic solution (30-70 °B). An approximate solution of Fick's law for unsteady state mass transfer in spherical configuration has been used to calculate the effective diffusion coefficients of water and sucrose. Results show that ohmic heating increases drastically the mass transfer and the effective diffusion rates. After 4 h of OD (without OH) in a sucrose solution (at 37 °C and 70 °B), the dry matter of the untreated strawberry halves was 20.3%; while it reached 68% when OD was combined with blanching by OH at 85 °C for 3-min. Ohmic blanching permits the effective damage of cells by combination of electrical and thermal effects. That result has an important enhancement of water and sugar transfers during osmotic dehydration of strawberries.

Ohmic heating (OH) of food has been investigated for many years as an alternative to conventional heating because it allows fast and homogeneous heating. The processing parameters that influence the most uniformity of the heating in OH are the electric field strength and the frequency. Therefore, recent trends have focused on studying the application of frequencies in the order of kHz and electric fields higher than 100 V/cm. In this regard, and considering only the applied field strength in a way to easily differentiate them, three ohmic systems could be distinguished: OH (< 100 V/cm), moderated electric fields (MEF) (100–1000 V/cm), and ohmic-pulsed electric fields (ohmic-PEF) (> 1000 V/cm). The advantages of applying higher electric fields (MEF and ohmic-PEF) over OH are, on the one hand, their much higher heating rate and, on the other hand, their capability to electroporate cells, causing the release of intracellular ionic compounds, and therefore, uniformizing the electrical conductivity of the product. This strategy is especially interesting for large solid foods where conventional heating applications lead to large temperature gradients and quality losses due to surface overtreatment. Therefore, the aim of this work is to review the state of the art of OH technologies, focusing on MEF and ohmic-PEF. The advantages and disadvantages of MEF and ohmic-PEF compared to OH and their potential for improving processes in the food industry are also discussed.

Ohmic heating or Joule heating has immense potential for achieving rapid and uniform heating in foods, providing microbiologically safe and high quality foods. This review discusses the technology behind ohmic heating, the current applications and thermal modeling of the process. The success of ohmic heating depends on the rate of heat generation in the system, the electrical conductivity of the food, electrical field strength, residence time and the method by which the food flows through the system. Ohmic heating is appropriate for processing of particulate and protein rich foods. A vast amount of work is still necessary to understand food properties in order to refine system design and maximize performance of this technology in the field of packaged foods and space food product development. Various economic studies will also play an important role in understanding the overall cost and viability of commercial application of this technology in food processing. Some of the demerits of the technology are also discussed.