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The mitotic-inhibiting herbicide pronamide controls susceptible annual bluegrass (Poa annua L.) pre- and postemergence, but in some resistant populations, postemergence activity is compromised, hypothetically due to a target-site mutation, lack of root uptake, or an unknown resistance mechanism. Three suspected pronamide-resistant (LH-R, SC-R, and SL-R) and two pronamide-susceptible (BS-S and HH-S) populations were collected from Mississippi golf courses. Dose–response experiments were conducted to confirm and quantify pronamide resistance, as well as resistance to flazasulfuron and simazine. Target sites known to confer resistance to mitotic-inhibiting herbicides were sequenced, as were target sites for herbicides inhibiting acetolactate synthase (ALS) and photosystem II (PSII). Pronamide absorption and translocation were investigated following foliar and soil applications. Dose–response experiments confirmed pronamide resistance of LH-R, SC-R, and SL-R populations, as well as instances of multiple resistance to ALS- and PSII-inhibiting herbicides. Sequencing of the α-tubulin gene confirmed the presence of a mutation that substituted isoleucine for threonine at position 239 (Thr-239-Ile) in LH-R, SC-R, SL-R, and BS-S populations. Foliar application experiments failed to identify differences in pronamide absorption and translocation between the five populations, regardless of harvest time. All populations had limited basipetal translocation—only 3% to 13% of the absorbed pronamide—across harvest times. Soil application experiments revealed that pronamide translocation was similar between SC-R, SL-R, and both susceptible populations across harvest times. The LH-R population translocated less soil-applied pronamide than susceptible populations at 24, 72, and 168 h after treatment, suggesting that reduced acropetal translocation may contribute to pronamide resistance. This study reports three new pronamide-resistant populations, two of which are resistant to two modes of action (MOAs), and one of which is resistant to three MOAs. Results suggest that both target site– and translocation-based mechanisms may be associated with pronamide resistance. Further research is needed to confirm the link between pronamide resistance and the Thr-239-Ile mutation of the α-tubulin gene.

Hair fescue is an economically destructive, tuft‐forming weed common in wild blueberry fields. The current management of hair fescue is almost completely reliant on pronamide (3,5‐dichloro‐N‐[2‐methylbut‐3‐yn‐2‐yl]benzamide) herbicide with few other products demonstrating effectiveness. Dichlobenil (2,6‐dichlorobenzonitrile) is a group 29 herbicide registered for use in wild blueberry; however, it has seen limited use because of its high product cost of over CAD 1800 ha−1 and limited research on the effect of varying application rates. Treatments (dichlobenil at 4400, 5700, and 7000 g a.i. ha−1, pronamide at 2240 g a.i. ha−1, and a nontreated control) were applied during the 2020 autumn prior to wild blueberry stem emergence in the nonbearing year (2021). Each treatment was replicated five times per field across three fields in central Nova Scotia. Dichlobenil at 4400, 5700, and 7000 g a.i. ha−1 reduced 2021 (nonbearing year) living tuft density by 59%, 71%, and 83%, respectively, and provided residual control in 2022 (bearing year) with densities reduced by 59%, 69%, and 78%, respectively. Comparatively, pronamide reduced 2021 and 2022 living tuft density by 95% and 91%. Reductions in living tuft density resulted in yield increases of 62%, 57%, and 94% in plots treated with dichlobenil at 4400, 5700, and 7000 g a.i. ha−1, respectively, and 73% for plots treated with pronamide. Results demonstrate that dichlobenil is an effective herbicide for managing hair fescue in wild blueberry. The potential for the selection of herbicide‐resistant biotypes to industry standard pronamide could be prolonged with integrated dichlobenil applications. Core Ideas Hair fescue is a yield‐limiting weed with few effective herbicide options in wild blueberry. Alternative chemicals with a different mode‐of‐action than pronamide are needed to prevent resistant grass selection. Dichlobenil at the highest labeled rate controlled hair fescue and increased wild blueberry yield. Rotating pronamide with dichlobenil should reduce the potential for herbicide‐resistant grass selection.

Annual bluegrass is one of the most problematic weeds in the turfgrass industry, exhibiting both cross-resistance and multiple-herbicide resistance. Prodiamine, pronamide, and indaziflam are commonly used preemergence herbicides for the control of this species on golf courses in the southern United States. There have been increasing anecdotal reports of annual bluegrass populations escaping control with these herbicides, but resistance has yet to be confirmed. To evaluate the response of annual bluegrass to three herbicides, populations were collected from golf courses, athletic fields, and landscape areas in Texas and Florida, and a dose-response assay was conducted on populations that were suspected to be resistant to and known to be susceptible to prodiamine, pronamide, and indaziflam. The suspected-resistant populations showed survival to prodiamine at 32 times the recommended field rate (both populations from Florida and Texas) of 736 g ai ha–1, and to pronamide at 32 times (the Florida populations) or 16 times (the Texas populations) the recommended field rate of 1,156 g ha–1. In contrast, the known susceptible populations attained 100% mortality at rates as low as 46 and 578 g ha–1, respectively, from applications of prodiamine and pronamide. For indaziflam, the suspected-resistant populations showed reduced sensitivity up to the recommended field rate of 55 g ha–1, but they were controlled when treated with a rate twice that of the field rate. Overall, annual bluegrass populations with resistance to prodiamine and pronamide, and reduced sensitivity to indaziflam (at the recommended field rate) were confirmed from golf courses in Florida and Texas. In the presence of herbicide-resistant annual bluegrass populations, especially to commonly used herbicides such as prodiamine and pronamide, turfgrass managers should adopt integrated management strategies and frequently rotate herbicide sites of action, rather than relying solely on microtubule-assembly inhibitors or cellulose biosynthesis inhibitors, to control this species. Nomenclature: Indaziflam; prodiamine; pronamide; annual bluegrass, Poa annua L.

Italian ryegrass [Lolium perenne L. spp. multiflorum (Lam.) Husnot] is one of the most troublesome weeds worldwide. Lolium multiflorum is also a grass seed crop cultivated on 50,000 ha in Oregon, where both diploid and tetraploid cultivars are grown. For this work, we will refer to the species as L. multiflorum, since the common names annual ryegrass and Italian ryegrass both refer to the same species. A survey was conducted to understand the distribution and frequency of L. multiflorum and its susceptibility to selected herbicides used in its control. The herbicides selected were clethodim, glufosinate, glyphosate, mesosulfuron-methyl (mesosulfuron), paraquat, pinoxaden, pyroxsulam, quizalofop-P-ethyl (quizalofop), pronamide, flufenacet + metribuzin, and pyroxasulfone. The ploidy levels of the populations were also tested. A total of 150 fields were surveyed between 2017 and 2018, of which 75 (50%) had L. multiflorum present. Herbicide-resistant populations were documented in 88% of the 75 populations collected. The most frequent resistances were to acetyl-CoA carboxylase (ACCase), acetolactate synthase (ALS), 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) inhibitors, and combinations thereof. Multiple resistance and cross-resistance, found in 75% of the populations, were the most frequent patterns of resistance. Paraquat-resistant biotypes were confirmed in six orchard crop populations for the first time in Oregon. Herbicide resistance was spatially clustered, with most cases of resistance in the northern part of the surveyed area. Populations resistant to ALS and ACCase inhibitors were prevalent in wheat (Triticum aestivum L.) fields. Multiple resistance was positively correlated with plant density. Tetraploid feral populations were identified, but no cases of herbicide resistance were documented. This is the first survey of herbicide resistance and ploidy diversity in L. multiflorum in western Oregon. Resistant populations were present across the surveyed area, indicating that the problem is widespread.

This study assessed the potential of using dichlobenil to manage hair fescue in lowbush blueberry crops when targeted or broadcast-applied (7,000 g ai ha −1 ) as justification for developing a precision-targeted applicator. A randomized complete block design was used to assess both application methods, and results were compared with industry-standard propanamide (2,240 g ai ha −1 ). Targeted and broadcast-applied dichlobenil in fall 2020 significantly reduced average total tuft density in the nonbearing year (2021) by 75% and 67%, respectively, and in the bearing year (2022) by 61% and 59%, respectively. Broadcast pronamide applications in fall 2020 significantly reduced total tuft density by 84% in the nonbearing year (2021) and 81% in the bearing year (2022). These reductions in total tuft density resulted in average lowbush blueberry yields of 416, 557, 573, and 617 g m −2 for the control, pronamide applications, and targeted and broadcast-applied dichlobenil, respectively. Increases in yield were not significant, though the large variation within the sample is the probable cause. The similarities between targeted and broadcast-applied treatments demonstrate the potential of using targeted dichlobenil. Given the high product cost of dichlobenil at Can$1,873 ha −1 , hair fescue’s non-uniform distribution in lowbush blueberry fields and the lowbush blueberry industry’s overreliance on pronamide, targeted application of dichlobenil has significant potential. This work justifies the development of a mechanized precision-targeted applicator for use in lowbush blueberry cropping systems.

Tetflupyrolimet is a novel herbicide that inhibits dihydroorotate dehydrogenase in susceptible weeds, including those in warm-season turfgrass and rice. Given that warm-season species are managed alongside cool-season species that may be sensitive to tetflupyrolimet, research on its lateral movement within turfgrass is warranted. Field experiments were conducted in spring 2023 and 2024 at North Carolina State University to evaluate the potential downslope movement of tetflupyrolimet (400 g ai ha −1 ) compared with that of pronamide (1,160 g ai ha − ¹), an herbicide that is known to move downslope. The studies took place on a 9.5% sloped plot of hybrid bermudagrass that had been established on Cecil sandy loam soil, under two moisture regimes at application: field capacity (≈34% volumetric water content) and saturation (≈46% volumetric water content). Before experimentation, the aboveground hybrid bermudagrass canopy was mechanically removed, and perennial ryegrass was planted as an indicator species. Herbicides were applied to treated areas (2.2 m 2 ) upslope of data collection areas (8.6 m 2 ), with subsequent irrigation and rainfall (2.5 cm total) 24 h after application. Downslope movement was assessed at 2, 4, 6, and 8 wk after treatment via perennial ryegrass mortality assessments made via grid (15 cm 2 ) count. Downslope distances associated with a 50% probability of perennial ryegrass mortality (mortality 50 ) were 1.2 to 3.6 times greater for pronamide compared to tetflupyrolimet. The maximum distance tetflupyrolimet moved was 1.1 m (regardless of soil moisture condition) each year. Comparatively, maximum downslope movement distance s for pronamide were 1.5 to 1.65 m under saturated conditions and 1.5 to 1.8 m at field capacity. Overall, these findings suggest a 1.1-m buffer from sensitive species is likely sufficient to prevent undesirable injury following tetflupyrolimet applications to hybrid bermudagrass under conditions similar to this study.

AIMS: This study was aimed at testing the hypothesis that lettuce corky root (CR) disease caused by Rhizorhapis suberifaciens was less severe in organic than conventional farms, due to the absence of herbicide and fertilizer, and greater soil microbial activity in organic farms. METHODS: CR severity and soil quality were assessed in pairs of conventional and organic farms in California. To determine factors contributing to CR, effects of N fertilizer and pronamide herbicide were assessed on CR severity and plant weight in separate field experiments. RESULTS: CR was significantly more severe in conventional than organic farms, and there was a negative exponential relationship between CR severity and microbial activity. Split applications of soluble N fertilizer enhanced susceptibility to CR compared to pre-plant application of slow release N fertilizer. Pronamide increased disease severity on seedlings compared to untreated controls and reduced the dry weights of seedlings and mature heads. CONCLUSIONS: Conventional practices, like fertilizer and herbicide use, increase plant susceptibility to and reduce microbial competition or antibiosis against R. suberifaciens in conventional lettuce production farms, potentially leading to enhanced environmental pollution due to a decrease in nutrient use efficiency and an increased need for fertilizer and water for diseased plants.

Annual bluegrass is a troublesome weed in turfgrass, with reported resistance to at least 12 herbicide sites of action. The mitotic-inhibiting herbicide pronamide has both preemergence and postemergence activity on susceptible annual bluegrass populations. Previous studies suggest that postemergence activity may be compromised due to lack of root uptake, as well as target-site- and translocation-based mechanisms. Research was conducted to determine the effects of spray droplet spectra on spray coverage and control of annual bluegrass with pronamide, flazasulfuron, and a mixture of pronamide plus flazasulfuron. Herbicides were delivered to annual bluegrass plants having two to three leaves via five different spray spectra based on volume median diameters (VMD) of 200, 400, 600, 800, and 1,000 µm. Fluorescent tracer dye was added to each treatment solution to quantify the effects of herbicide and spray droplet spectra on herbicide deposition. In another experiment, the efficacy of 0.58, 1.16, and 2.32 kg pronamide ha–1; 0.022, 0.044, and 0.088 kg flazasulfuron ha–1, or a combination of the two, were assessed in iteration with droplet spectrum sprays of 400 and 1,000 µm on two pronamide-resistant and two pronamide-susceptible annual bluegrass populations. Spray droplet spectrum affected the deposition of pronamide and flazasulfuron, applied alone and in combination. Pronamide foliar deposition decreased with increasing droplet spectra. Pronamide efficacy was affected by droplet spectrum, with the largest (1,000 µm) exhibiting improved control. Flazasulfuron efficacy and pronamide plus flazasulfuron efficacy were not affected by droplet spectra. Pronamide plus flazasulfuron mixture controlled all four populations more effectively than pronamide alone, regardless of droplet spectra. A mixture of pronamide plus flazasulfuron applied with relatively large droplets may be optimal for annual bluegrass control, which offers valuable insights for optimizing herbicide application and combatting herbicide resistance. However, applications in this controlled-growth pot study may not mimic conditions in which thatch and turfgrass canopy limit the soil deposition of pronamide. Nomenclature: Flazasulfuron; pronamide; propyzamide; annual bluegrass; Poa annua L.; POAN

The performance of the Robovator (F. Poulsen Engineering ApS, Hvalsø, Denmark), a commercial robotic intrarow cultivator, was evaluated in direct-seeded broccoli and transplanted lettuce during 2014 and 2015 in Salinas, CA, and Yuma, AZ. The main objective was to evaluate the crop stand after cultivation, crop yield, and weed control efficacy of the Robovator compared with a standard cultivator. A second objective was to compare hand weeding time after cultivation within a complete integrated weed management (IWM) system. Herbicides were included as a component of the IWM system. The Robovator did not reduce crop stand or marketable yield compared with the standard cultivator. The Robovator removed 18 to 41% more weeds at moderate to high weed densities and reduced hand-weeding times by 20 to 45% compared with the standard cultivator. At low weed densities there was little difference between the cultivators in terms of weed control and hand-weeding times. The lower-hand weeding time with the Robovator treatments suggest that robotic intrarow cultivators can reduce dependency on hand weeding compared with standard cultivators. Technological advancements and price reductions of these types of machines will likely improve their weed removal efficacy and the long-term viability of IWM programs that will use them. Nomenclature: Broccoli, Brassica oleracea L. ‘Marathon'; lettuce, Lactuca sativa L. ‘Sunbelt'. El desempeño del Robovator (F. Poulsen Engineering ApS, Hvalsø, Denmark), un cultivador robótico comercial para uso dentro de las hileras de siembra, fue evaluado en brócoli de siembra directa y lechuga trasplantada durante 2014 y 2015 en Salinas, California y Yuma, Arizona. El objetivo principal fue evaluar el cultivo establecido después de la labranza, el rendimiento del cultivo, y la eficacia para el control de malezas del Robovator, al compararse con un cultivador estándar. Un segundo objetivo fue comparar el tiempo de deshierba manual después de la labranza dentro de un sistema de manejo integrado de malezas (IWM) completo. Se incluyó herbicidas como un componente del sistema IWM. El Robovator no redujo el número de plantas del cultivo establecidas ni el rendimiento comercializable al compararse con el cultivador estándar. El Robovator eliminó 18 a 41% más malezas en densidades de moderadas a altas y redujo el tiempo de deshierba manual en 30 a 45% al compararse con el cultivador estándar. A bajas densidades hubo pocas diferencias entre los cultivadores en términos de control de malezas y tiempos de deshierba manual. El mejor tiempo de deshierba manual con los tratamientos con Robovator sugiere que cultivadores robóticos para uso dentro de las hileras de siembra pueden reducir la dependencia en la deshierba manual en comparación con cultivadores estándar. Los avances tecnológicos y las reducciones en precio de este tipo de máquinas probablemente mejorará la eficacia en la remoción de malezas y la viabilidad en el largo plazo de los programas IWM que los usen.

Successful weed management, particularly use of chemical control, is very important for commercial lettuce production on organic soils in the Everglades Agricultural Area in south Florida. Field experiments were conducted in 2016 and 2017 to determine the efficacy of herbicides (pronamide, bensulide, imazethapyr, or oxyfluorfen) applied preemergence (PRE) either alone or followed by a postemergence (POST) application of imazethapyr for weed control and lettuce (romaine and iceberg) yield. Preemergence-applied oxyfluorfen (0.56 kg ha–1) resulted in significant lettuce injury, including stand loss, while PRE applications of pronamide (4.44 kg ha–1), bensulide (5.6 and 10.1 kg ha–1), or imazethapyr (0.035 g ha–1) resulted in transient lettuce injury and no significant stand loss. Similarly, PRE-applied pronamide, bensulide, and imazethapyr followed by POST-applied imazethapyr did not result in significant lettuce stand loss or injury. When contrasted as a group, PRE-applied herbicides followed by a POST application of imazethapyr provided better spiny amaranth and common lambsquarters control compared with PRE-applied herbicides or POST-applied imazethapyr-only treatments. Lettuce yield was highest with PRE herbicides followed by POST imazethapyr compared with PRE herbicides or POST-applied imazethapyr-only treatments, indicating a yield benefit of having a PRE followed by POST herbicide weed control program in lettuce grown on organic soils. However, oxyfluorfen is not an option for lettuce on organic soils because of unacceptable stand reduction and crop injury. Whether to apply pronamide, bensulide, or imazethapyr PRE followed by a POST application of imazethapyr for broadleaf weed control in lettuce on organic soils depends on the species present, cost, and ease of application. Nomenclature: Bensulide; imazethapyr; oxyfluorfen; pronamide; lettuce, Lactuca sativa L.; common lambsquarters, Chenopodium album L. CHEAL; spiny amaranth, Amaranthus spinosus L. AMASP