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Purpose The purpose of this study is to model and solve numerically the three-dimensional off-centered stagnation point flow and heat transfer of magnesium oxide–silver/water hybrid nanofluid impinging to a spinning disk. Design/methodology/approach The applied effective thermophysical properties of hybrid nanofluid including thermal conductivity and dynamics viscosity are according to the reported experimental relations that would be expanded by a mass-based algorithm. The single phase formulations coupled with experimental-based hybrid nanofluid model is implemented to derive the governing partial differential equations which are then transferred to a set of dimensionless ordinary differential equations (ODEs) with the use of the similarity transformation method. Afterward, the reduced ODEs are solved numerically by bvp4c function from MATLAB that is a trustworthy and efficient code according to three-stage Lobatto IIIa formula. Findings The effect of spinning parameter and nanoparticles masses (mMgO, mAg) on the hydrodynamics and thermal boundary layers behavior and also the quantities of engineering interest are presented in tabular and graphical forms. The recent work demonstrates that the analysis of flow and heat transfer becomes more complicated when there is a non-alignment between the impinging flow and the disk axes. From computational results demonstrate that, the radial and azimuthal velocities are, respectively, the increasing and decreasing functions of the disk spinning parameter. Further, for the greater values of the spinning parameter, an overshoot of the radial velocity owing to the centrifugal forces of the spinning disk is observed. Besides, the quantities of engineering interest gently enhance with first and second nanoparticle masses, while comparing their absolute values illustrates the fact that the effect of second nanoparticle mass (mAg) is greater. Further, it is inferred that the second nanoparticle’s mass enhancement results in the amplification of the heat transfer; although, the high skin friction and the relevant shear stress should be controlled. Originality/value The combination of experimental thermophysical properties with theoretical modeling of the problem can be the novelty of the present work. It is evident that the experimental relations of effective thermophysical properties can be trustable and flexible in the theoretical/mathematical modeling of hybrid nanofluids flows. Besides, to the best of the authors’ knowledge, no one has ever attempted to study the present problem through a mass-based model for hybrid nanofluid.

Three-dimensional imaging cannot be achieved easily using previously developed localization super-resolution techniques. Here, we present a three-dimensional multimodal sub-diffraction imaging technique with spinning-disk （SD） confocal microscopy called 3D-MUSIC, which not only has all the advantages of SD confocal microscopy such as fast imaging speed, high signal-to-noise ratio, and optical-sectioning capability, but also extends its spatial resolution limit along all three dimensions. Both axial and lateral resolution can be improved simul- taneously by virtue of the blinking/fluctuating nature of modified fluorescent probes, exemplified with the quantum dots. Further, super-resolution images with dual modality can be obtained through super-resolution optical fluctuation imaging （SOFI） and bleaching/blinking-assisted localization microscopy （BALM）. Therefore, fast super-resolution imaging can be achieved with SD-SOFI by capturing only 100 frames while SD-BaLM yields high-resolution imaging.

The friction-induced vibration of a mass–slider with in-plane and transverse springs and dampers in sliding contact with a spinning elastic disc in three different situations of spinning speed, i.e. constant deceleration, constant acceleration and constant speed, is studied. The stick–slip motion in the circumferential direction and separation–re-contact behaviour in the transverse direction are considered, which make the system responses non-smooth. It is observed that the decelerating rotation of the disc can make the in-plane stick–slip motion of the slider more complicated in comparison with constant disc rotation and thereby exerting significant influence on the transverse vibration of the disc, while the accelerating rotation of the disc contributes to the occurrence of separation during the vibration and thus influencing the vibration behaviour of the system. Numerical simulation results show that distinct dynamic behaviours can be observed in the three situations of spinning speed of disc and three kinds of particular characteristics of differences are revealed. The significant effects of decelerating and accelerating disc rotation on the friction-induced dynamics of the system underlie the necessity to consider the time-variant spinning speed of disc in the research of friction-induced vibration and noise.

We demonstrate how a conventional confocal spinning-disk (CSD) microscope can be converted into a doubly resolving image scanning microscopy (ISM) system without changing any part of its optical or mechanical elements. Making use of the intrinsic properties of a CSD microscope, we illuminate stroboscopically, generating an array of excitation foci that are moved across the sample by varying the phase between stroboscopic excitation and rotation of the spinning disk. ISM then generates an image with nearly doubled resolution. Using conventional fluorophores, we have imaged single nuclear pore complexes in the nuclear membrane and aggregates of GFP-conjugated Tau protein in three dimensions. Multicolor ISM was shown on cytoskeletal-associated structural proteins and on 3D four-color images including MitoTracker and Hoechst staining. The simple adaptation of conventional CSD equipment allows superresolution investigations of a broad variety of cell biological questions.

Spinning disk confocal microscopy enables fast optical sectioning with low phototoxicity but is often inaccessible due to high hardware costs. We present a lower-cost solution using a 25-megapixel machine vision CMOS camera and a custom-built spinning disk. This camera uses a back-illuminated sensor with high quantum efficiency and low read noise. High-resolution images of Thy1-GFP mouse brain slices, Drosophila embryos and larvae, and H&E-stained rat testis verified performance across 3D tissue volumes. The measured resolution was 215.8 nm in X, Y and 521.9 nm in Z with a 60×/1.42 NA objective. The custom disk, made with 18 µm pinholes (180 µm pitch) on a chrome photomask and mounted to an optical chopper motor, enables stable, near-telecentric imaging at lower magnifications. Micromanager software integration allows synchronized control of all hardware, which demonstrates that affordable CMOS sensors can potentially replace sCMOS in spinning disk microscopy, offering an open-access, scalable solution for advanced imaging.

Mitochondria are the energy factories of a cell, and depending on the metabolic requirements, the mitochondrial morphology, quantity, and membrane potential in a cell change. These changes are frequently assessed using commercially available probes. In this study, we tested the suitability of three commercially available probes—namely 5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolo-carbocyanine iodide (JC-1), MitoTracker Red CMX Rox (CMXRos), and tetramethylrhodamine methyl ester (TMRM)—for assessing the mitochondrial quantity, morphology, and membrane potential in living human mesoangioblasts in 3D with confocal laser scanning microscope (CLSM) and scanning disk confocal microscope (SDCM). Using CLSM, JC-1, and CMXRos—but not TMRM—uncovered considerable background and variation. Using SDCM, the background signal only remained apparent for the JC-1 monomer. Repetitive imaging of CMXRos and JC-1—but not TMRM—demonstrated a 1.5–2-fold variation in signal intensity between cells using CLSM. The use of SDCM drastically reduced this variation. The slope of the relative signal intensity upon repetitive imaging using CLSM was lowest for TMRM (−0.03) and highest for CMXRos (0.16). Upon repetitive imaging using SDCM, the slope varied from 0 (CMXRos) to a maximum of −0.27 (JC-1 C1). Conclusively, our data show that TMRM staining outperformed JC-1 and CMXRos dyes in a (repetitive) 3D analysis of the entire mitochondrial quantity, morphology, and membrane potential in living cells.

The analysis explored a numerical simulation of microorganisms, carbon nanotubes (CNTs) and ferric oxide water-based hybrid nanofluid flow induced by a wavy fluctuating spinning disc with energy propagation. In the presence of CNTs and magnetic nanoparticulates, the nanofluid is synthesized. The exceptional tensile strength, flexibility, and electrical and thermal conductivity of carbon nanotubes and iron nanoparticles have been extensively reported. The motive of the proposed analysis is to optimize thermal energy conveyance efficiency for a spectrum of industrial and biomedical applications. The phenomena have been expressed as a system of partial differential equations (PDEs) which contain the momentum, energy, concentration, and motile microorganism equations. The modeled equations have been diminished to the dimensionless system of nonlinear ODEs through a similarity framework. The Matlab built-in package boundary value solver has been utilized to solve the obtained system of ODEs. The findings are compared to the PCM technique for validity purposes. The results are illustrated graphically and discussed. The layout of a rotating disc has a positive effect on energy transition and velocity profile. The irregular rotating surface increases energy progression up to 15% relative to a smooth surface. The accumulation of nanocomposites (CNTs and magnetic nanoparticles) significantly enhanced the thermal capabilities of the liquid medium. When operating with a low distribution, it is more impactful.

This paper is concerned about the characteristics of the dual solutions of an unsteady stagnation-point flow of a hybrid nanofluid over a rotating disk with the effect of Hall current. The governing equations are non-dimensionalized using the appropriate similarity transformations. The resulting equations are solved numerically by employing the bvp4c method. A comparison between the present solutions with the available solutions is presented which shows an excellent agreement. The solution exists in the upper branch for all values of the hybrid nanofluid ϕ 1 , ϕ 2 , the swirl parameter Ω A , and impinging parameter β A . However, the breakdown is observed in the lower branch solution at some moderate values of β A . In addition, the radial velocity increases due to hybrid nanofluid, while the azimuthal velocity decreases. However, the temperature increases due to hybrid nanofluid. Moreover, the results also indicate that the swirl parameter augments the velocity in both directions but declines the temperature. Also, it is perceived that the azimuthal velocity and temperature decrease in the upper branch solution as the magnetic parameter increases, while the lower branch solution initially increases and then decreases for the transverse component of velocity but the temperature monotonically increases.

The measurement of dynamic changes in protein level and localization throughout the cell cycle is of major relevance to studies of cellular processes tightly coordinated with the cycle, such as replication, transcription, DNA repair, and checkpoint control. Currently available methods include biochemical assays of cells in bulk following synchronization, which determine protein levels with poor temporal and no spatial resolution. Taking advantage of genetic engineering and live-cell microscopy, we performed time-lapse imaging of cells expressing fluorescently tagged proteins under the control of their endogenous regulatory elements in order to follow their levels throughout the cell cycle. We effectively discern between cell cycle phases and S subphases based on fluorescence intensity and distribution of co-expressed proliferating cell nuclear antigen (PCNA)-mCherry. This allowed us to precisely determine and compare the levels and distribution of multiple replication-associated factors, including Rap1-interacting factor 1 (RIF1), minichromosome maintenance complex component 6 (MCM6), origin recognition complex subunit 1 (ORC1, and Claspin, with high spatiotemporal resolution in HeLa Kyoto cells. Combining these data with available mass spectrometry-based measurements of protein concentrations reveals the changes in the concentration of these proteins throughout the cell cycle. Our approach provides a practical basis for a detailed interrogation of protein dynamics in the context of the cell cycle.

The circadian pacemaker in the hypothalamic suprachiasmatic nucleus (SCN) is a hierarchical multioscillator system in which neuronal networks play crucial roles in expressing coherent rhythms in physiology and behavior. However, our understanding of the neuronal network is still incomplete. Intracellular calcium mediates the input signals, such as phase-resetting stimuli, to the core molecular loop involving clock genes for circadian rhythm generation and the output signals from the loop to various cellular functions, including changes in neurotransmitter release. Using a unique large-scale calcium imaging method with genetically encoded calcium sensors, we visualized intracellular calcium from the entire surface of SCN slice in culture including the regions where autonomous clock gene expression was undetectable. We found circadian calcium rhythms at a single-cell level in the SCN, which were topologically specific with a larger amplitude and more delayed phase in the ventral region than the dorsal. The robustness of the rhythm was reduced but persisted even after blocking the neuronal firing with tetrodotoxin (TTX). Notably, TTX dissociated the circadian calcium rhythms between the dorsal and ventral SCN. In contrast, a blocker of gap junctions, carbenoxolone, had only a minor effect on the calcium rhythms at both the single-cell and network levels. These results reveal the topological specificity of the circadian calcium rhythm in the SCN and the presence of coupled regional pacemakers in the dorsal and ventral regions. Neuronal firings are not necessary for the persistence of the calcium rhythms but indispensable for the hierarchical organization of rhythmicity in the SCN.