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To determine the factors leading to outbreaks of the sugarcane aphid, Melanaphis sacchari, (Zehntner) (Hemiptera: Aphididae) in sorghum in Haiti, a survey was carried out on farms during two cropping seasons, spring and fall of 2018. A total of 45 plots in three Haitian regions were monitored from the five-leaf stage to grain ripening. Infestation with M. sacchari was significantly higher in spring than in fall, except in one location. Melanaphis sacchari populations varied significantly according to phenological stages of sorghum, with significantly higher abundance during the heading and flowering stages than other stages. In and around sorghum fields, the sugarcane aphid was observed on plants from three families: Poaceae, Malvaceae, and Cucurbitaceae. Aphid natural enemies found in sorghum fields consisted of eight species of ladybeetles (Coleoptera: Coccinellidae), one hoverfly (Diptera: Syrphidae), one lacewing (Neuroptera: Chrysopidae), and one predatory midge (Diptera: Cecidomyiidae). In addition to these predators, two parasitoid species, Adialytus sp. (Hymenoptera: Braconidae) and Pachyneuron aphidis (Bouché) (Hymenoptera: Pteromalidae), emerged from sugarcane aphid mummies. Predator and parasitoid densities were highly affected by growing season, with most of the biological control happening in fall. This study provides insights on sugarcane aphid management in Haiti and the Caribbean Islands. L'objectif de cette étude est de déterminer les facteurs pouvant conduire à une infestation des champs de sorgho en Haïti par le puceron de la canne à sucre Melanaphis sacchari, (Zehntner) (Hemiptera: Aphididae). L'étude a été conduite dans des exploitations paysannes pendant deux saisons, au printemps et à l'automne 2018. Au total, 45 champs localisés dans trois régions furent suivis depuis le stage à cinq feuilles du sorgho jusqu'à la maturation des graines. Les infestations de M. sacchari étaient plus élevées au printemps qu'en automne, sauf dans une région. Les populations de M. sacchari fluctuèrent de façon significative selon le stage phénologique du sorgho, avec des abondances plus élevées pendant l'épiaison et la floraison que pendant les autres stages. A l'intérieur et à l'extérieur des champs de sorgho, M. sacchari fut observé sur des plantes de trois familles: Poaceae, Malvaceae, et Cucurbitaceae. Les ennemis naturels des pucerons trouvés dans les champs de sorgho consistaient en huit espèces de coccinelles (Coleoptera: Coccinellidae), une espèce de syrphe (Diptera: Syrphidae), une espèces de chrysope (Neuroptera: Chrysopidae), et une espèce de diptère prédatrice (Diptera: Cecidomyiidae). En plus de ces prédateurs, deux parasitoïdes Adialytus sp. (Hymenoptera: Braconidae) et Pachyneuron aphidis (Bouché) (Hymenoptera: Pteromalidae), émergèrent des momies de pucerons. Les densités de prédateurs et de parasitoïdes furent grandement affectées par la saison de culture avec la majorité de leur présence à l'automne. Cette étude donne les premiers éléments concernant le contrôle du puceron de la canne à sucre en Haïti and dans les Caraïbes.

Main conclusionInsect herbivores of different feeding guilds induced sorghum defenses through differential mechanisms, regardless of the order of herbivore arrival on sorghum plants.Sorghum, one of the world’s most important cereal crops, suffers severe yield losses due to attack by insects of different feeding guilds. In most instances, the emergence of these pests are not secluded incidents and are followed by another or can also co-infest host plants. Sugarcane aphid (SCA) and fall armyworm (FAW) are the two most important destructive pests of sorghum, which belongs to sap-sucking and chewing feeding guilds, respectively. While the order of the herbivore arriving on the plants has been found to alter the defense response to subsequent herbivores, this is seldom studied with herbivores from different feeding guilds. In this study, we investigated the effects of sequential herbivory of FAW and SCA on sorghum defense responses and their underlying mechanism(s). Sequential feeding on the sorghum RTx430 genotype by either FAW primed–SCA or SCA primed–FAW were monitored to unravel the mechanisms underlying defense priming, and its mode of action. Regardless of the order of herbivore arrival on sorghum RTx430 plants, significant defense induction was observed in the primed state compared to the non-primed condition, irrespective of their feeding guild. Additionally, gene expression and secondary metabolite analysis revealed differential modulation of the phenylpropanoid pathway upon insect attack by different feeding guilds. Our findings suggest that priming in sorghum plants upon sequential herbivory induces defense by the accumulation of the total flavonoids and lignin/salicylic acid in FAW primed–SCA and SCA primed–FAW interaction, respectively.

The sugarcane aphid, Melanaphis sacchari (Zehntner), emerged as a severe pest of sorghum, Sorghum bicolor (L.), in Texas and Louisiana in 2013 and currently threatens nearly all sorghum production in the United States. Proper management of populations is critical as sugarcane aphid has a high reproductive potential and can rapidly damage plants, resulting in extensive yield losses. The overall objective of this work was to investigate sugarcane aphid population dynamics, and subsequent sorghum injury and grain yield on commercially available grain sorghum varieties in Alabama. This research includes three-site years of data that show variation in plant injury, physiological maturity, and yields among varieties tested. Although performance of each variety was variable among locations, potentially due to abiotic factors, four varieties including DKS 37-07, 1G588, 1G855, and 83P17 exhibited characteristics consistent with resistance and corroborates reports of resistance from other states.

The invasive sugarcane aphid, Melanaphis sacchari (Zehntner), is a devastating new pest of grain sorghum. Studies were conducted utilizing an integrated approach of four management tactics: planting date, insecticidal seed treatment, a foliar-applied insecticide, and plant resistance. Experiments were conducted in 2016 and 2017 at Griffin, Tifton, and Plains Georgia, and in 2016 in Texas, Alabama, and Oklahoma, United States. Early planting was effective in reducing damage and increasing yields when compared to the late planting. Use of a resistant variety reduced cumulative aphid-days, plant injury and usually prevented significant yield loss. Foliar application of flupyradifurone when aphids reached an economic threshold, was an effective management tactic preventing aphid injury and yield loss. Use of clothianidin seed treatment also reduced aphid injury and yield loss of the susceptible hybrid but generally did not prevent injury and yield loss of the resistant hybrid. We conclude that an earlier planting date coupled with a resistant variety and judicious use of an efficacious foliar-applied insecticide can effectively manage sugarcane aphid on grain sorghum. An insecticide seed treatment also may be useful to reduce the risk of sugarcane aphid damage to seedlings of susceptible hybrids.

The graminous host range and sources of sorghum [Sorghum bicolor (L.) Moench.] plant resistance, including cross-resistance from greenbug, Schizaphis graminum (Rondani), were studied for the newly emerging sugarcane aphid, Melanaphis sacchari (Zehntner), in greenhouse no-choice experiments and field evaluations. The sugarcane aphid could not survive on field corn, Zea mays (L.), Teff grass, Eragrostis tef (Zucc.), proso millet, Panicum miliaceum L., barley, Hordeum vulgare L., and rye, Secale cereale L. Only sorghum genotypes served as hosts including Johnsongrass, Sorghum halepense (L.), a highly suitable noncrop host that generates high numbers of sugarcane aphid and maintains moderate phenotypic injury. The greenbug-resistant parental line RTx2783 that is resistant to greenbug biotypes C and E was resistant to sugarcane aphid in both greenhouse and field tests, while PI 55607 greenbug resistant to biotypes B, C, and E was highly susceptible. PI 55610 that is greenbug resistant to biotypes B, C, and E maintained moderate resistance to the sugarcane aphid, while greenbug-resistant PI 264453 was highly susceptible to sugarcane aphid. Two lines and two hybrids from the Texas A&M breeding program B11070, B11070, AB11055-WF1-CS1/RTx436, and AB11055-WF1-CS1/RTx437 were highly resistant to sugarcane aphid, as were parental types SC110, SC170, and South African lines Ent62/SADC, (Macia/TAM428)-LL9, (SV1*Sima/IS23250)-LG15. Tam428, a parental line that previously showed moderate resistance in South Africa and India, also showed moderate resistance in these evaluations. Overall, 9 of 20 parental sorghum entries tested for phenotypic damage in the field resulted in good resistance to the sugarcane aphid and should be utilized in breeding programs that develop agronomically acceptable sorghums for the southern regions of the United States.

Background The sugarcane aphid (SCA; Melanaphis sacchari ) has emerged as a key pest on sorghum in the United States that feeds from the phloem tissue, drains nutrients, and inflicts physical damage to plants. Previously, it has been shown that SCA reproduction was low and high on sorghum SC265 and SC1345 plants, respectively, compared to RTx430, an elite sorghum male parental line (reference line). In this study, we focused on identifying the defense-related genes that confer resistance to SCA at early and late time points in sorghum plants with varied levels of SCA resistance. Results We used RNA-sequencing approach to identify the global transcriptomic responses to aphid infestation on RTx430, SC265, and SC1345 plants at early time points 6, 24, and 48 h post infestation (hpi) and after extended period of SCA feeding for 7 days. Aphid feeding on the SCA-resistant line upregulated the expression of 3827 and 2076 genes at early and late time points, respectively, which was relatively higher compared to RTx430 and SC1345 plants. Co-expression network analysis revealed that aphid infestation modulates sorghum defenses by regulating genes corresponding to phenylpropanoid metabolic pathways, secondary metabolic process, oxidoreductase activity, phytohormones, sugar metabolism and cell wall-related genes. There were 187 genes that were highly expressed during the early time of aphid infestation in the SCA-resistant line, including genes encoding leucine-rich repeat (LRR) proteins, ethylene response factors, cell wall-related, pathogenesis-related proteins, and disease resistance-responsive dirigent-like proteins. At 7 days post infestation (dpi), 173 genes had elevated expression levels in the SCA-resistant line and were involved in sucrose metabolism, callose formation, phospholipid metabolism, and proteinase inhibitors. Conclusions In summary, our results indicate that the SCA-resistant line is better adapted to activate early defense signaling mechanisms in response to SCA infestation because of the rapid activation of the defense mechanisms by regulating genes involved in monolignol biosynthesis pathway, oxidoreductase activity, biosynthesis of phytohormones, and cell wall composition. This study offers further insights to better understand sorghum defenses against aphid herbivory.

The aphid Melanaphis sacchari/sorghi is considered the most important pest of sorghum cultivation in Mexico. It can cause losses in production of up to 100%. This research was conducted at the Universidad Autonoma Chapingo during 2018 and 2019 with the objective of determining the relationship between chemical fertilization in sorghum and M. sacchari/sorghi (Hemiptera: Aphididae) populations. Different levels of nitrogen (125, 250 and 500 kg ha-1), phosphorus (19.5, 39 and 78 kg ha-1) and potassium (210 and 420 kg ha-1) were supplied to sorghum plants planted in polyethylene pots with a capacity of 6 L under greenhouse conditions. The plants were infested with a total of 15 third-instar nymphs within 60 days of crop emergence. A total of 6 samplings were carried out at 7 day intervals, starting at 7 d after the infestation. Quantifications of total soluble proteins, total soluble sugars, reducing sugars, and levels of nitrogen, phosphorus, potassium and magnesium in leaf tissue were also performed. We observed that higher doses of nitrogen and phosphorus increased the aphid population, while increasing the potassium dose considerably decreased the aphid population. We also found a positive correlation between the aphid population and the total soluble protein concentrations, reducing sugars and nitrogen levels, while the correlation was negative with potassium levels in leaves.

The sugarcane aphid ( ) has emerged as a significant pest for sorghum. The use of sugarcane aphid-resistant sorghum germplasm with integrated pest management strategies appears to be an excellent solution to this problem. In this study, a resistant line (RTx2783) and a susceptible line (A/BCK60) were used to characterize the differences in plant responses to the sugarcane aphid through a series of experiments, which examined global sorghum gene expression, aphid feeding behavior and inheritance of aphid resistance. The global transcriptomic responses to sugarcane aphids in resistant and susceptible plants were identified using RNA-seq and compared to the expression profiles of uninfested plants at 5, 10, and 15 days post-infestation. The expression of genes from several functional categories were altered in aphid-infested susceptible plants, which included genes related to cell wall modification, photosynthesis and phytohormone biosynthesis. In the resistant line, only 31 genes were differentially expressed in the infested plants relative to uninfested plants over the same timecourse. However, network analysis of these transcriptomes identified a co-expression module where the expression of multiple sugar and starch associated genes were repressed in infested resistant plants at 5 and 10 days. Several nucleotide-binding-site, leucine-rich repeat (NBS-LRR) and disease resistance genes similar to aphid resistance genes identified in other plants are identified in the current study which may be involved in sugarcane aphid resistance. The electrical penetration graph (EPG) results indicated that sugarcane aphid spent approximately twice as long in non-probing phase, and approximately a quarter of time in phloem ingestion phase on the resistant and F plants compared to susceptible plant. Additionally, network analysis identified a phloem protein 2 gene expressed in both susceptible and resistant plants early (day 5) of infestation, which may contribute to defense against aphid feeding within sieve elements. The resistant line RTx2783 displayed both antixenosis and antibiosis modes of resistance based on EPG and choice bioassays between susceptible, resistant and F plants. Aphid resistance from RTx2783 segregated as a single dominant locus in the F generation, which will enable breeders to rapidly develop sugarcane aphid-resistant hybrids using RTx2783 as the male parent.

Sugarcane aphid has emerged as a major pest of sorghum recently, and a few sorghum accessions were identified for resistance to this aphid so far. However, the molecular and genetic mechanisms underlying this resistance are still unclear. To understand these mechanisms, transcriptomics was conducted in resistant Tx2783 and susceptible BTx623 sorghum genotypes infested with sugarcane aphids. A principal component analysis revealed differences in the transcriptomic profiles of the two genotypes. The pathway analysis of the differentially expressed genes (DEGs) indicated the upregulation of a set of genes related to signal perception (nucleotide-binding, leucine-rich repeat proteins), signal transduction [mitogen-activated protein kinases signaling, salicylic acid (SA), and jasmonic acid (JA)], and plant defense (transcription factors, flavonoids, and terpenoids). The upregulation of the selected DEGs was verified by real-time quantitative PCR data analysis, performed on the resistant and susceptible genotypes. A phytohormone bioassay experiment showed a decrease in aphid population, plant mortality, and damage in the susceptible genotype when treated with JA and SA. Together, the results indicate that the set of genes, pathways, and defense compounds is involved in host plant resistance to aphids. These findings shed light on the specific role of each DEG, thus advancing our understanding of the genetic and molecular mechanisms of host plant resistance to aphids.

The sugarcane aphid, Melanaphis sacchari, is an agricultural pest that causes damage to plants in the Poaceae (the grasses) family, such as sorghum and sugarcane. In this study, we used nanopore long reads and a high-throughput chromosome conformation capture chromatin interaction maps to generate a chromosome-level assembly with a total length of 356.1 Mb, of which 85.5% (304.6 Mb) is contained within the 3 autosomes and the X chromosome. Repetitive sequences accounted for 16.29% of the chromosomes, and a total of 12,530 protein-coding genes were annotated, achieving 95.8% Benchmarking Universal Single-Copy Ortholog gene completeness. This offered a substantial improvement compared with previous low-quality genomic resources. A phylogenomic analysis by comparing M. sacchari with 24 published aphid genomes representing 3 aphid tribes revealed that M. sacchari belonged to the tribe Aphidini and maintained a conserved chromosome structure with other Aphidini species. The high-quality genomic resources reported in this study are useful for understanding the evolution of aphid genomes and studying pest management of M. sacchari.