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Durability is the most pressing issue preventing the efficient commercialization of polymer electrolyte membrane fuel cell (PEMFC) stationary and transportation applications. A big barrier to overcoming the durability limitations is gaining a better understanding of failure modes for user profiles. In addition, durability test protocols for determining the lifetime of PEMFCs are important factors in the development of the technology. These methods are designed to gather enough data about the cell/stack to understand its efficiency and durability without causing it to fail. They also provide some indication of the cell/stack’s age in terms of changes in performance over time. Based on a study of the literature, the fundamental factors influencing PEMFC long-term durability and the durability test protocols for both PEMFC stationary and transportation applications were discussed and outlined in depth in this review. This brief analysis should provide engineers and researchers with a fast overview as well as a useful toolbox for investigating PEMFC durability issues.

The novel coronavirus SARS-CoV-2 is the causative agent of the acute respiratory disease COVID-19, which has become a global concern due to its rapid spread. Meanwhile, increased demand for testing has led to a shortage of reagents and supplies and compromised the performance of diagnostic laboratories in many countries. Both the World Health Organization (WHO) and the Center for Disease Control and Prevention (CDC) recommend multi-step RT-PCR assays using multiple primer and probe pairs, which might complicate the interpretation of the test results, especially for borderline cases. In this study, we describe an alternative RT-PCR approach for the detection of SARS-CoV-2 RNA that can be used for the probe-based detection of clinical isolates in diagnostics as well as in research labs using a low-cost SYBR green method. For the evaluation, we used samples from patients with confirmed SARS-CoV-2 infections and performed RT-PCR assays along with successive dilutions of RNA standards to determine the limit of detection. We identified an M-gene binding primer and probe pair highly suitable for the quantitative detection of SARS-CoV-2 RNA for diagnostic and research purposes.

The present study aims to develop a test protocol based on the literature for electrochemical characterisation of a polymer electrolyte membrane (PEM) electrolysis commercial stack using polarisation curves. For this, a 1-kW water electrolysis test stand with integrated temperature control and measurement systems was built around the stack. Afterwards, the stack performance was characterised under different operating pressure and temperature conditions by using polarisation curves. A measurement protocol was developed based on the literature. To ensure the reproducibility of the results, two rounds of experiments were performed. The experiments were carried out at temperatures between 20 and 60 °C and pressures up to 15 bar. The results show distinct regions in the polarisation curves related to the activation and ohmic overvoltage. The effect of temperature and pressure on the performance is shown and analysed. The performance of single cells in the stack is also measured. The stack polarisation curves are compared with those in the literature, which gives an understanding of the materials used in electrodes and types of membranes.

Research on external human–machine interfaces (eHMIs) has recently become a major area of interest in the field of human factors research on automated driving. The broad variety of methodological approaches renders the current state of research inconclusive and comparisons between interface designs impossible. To date, there are no standardized test procedures to evaluate and compare different design variants of eHMIs with each other and with interactions without eHMIs. This article presents a standardized test procedure that enables the effective usability evaluation of eHMI design solutions. First, the test procedure provides a methodological approach to deduce relevant use cases for the evaluation of an eHMI. In addition, we define specific usability requirements that must be fulfilled by an eHMI to be effective, efficient, and satisfying. To prove whether an eHMI meets the defined requirements, we have developed a test protocol for the empirical evaluation of an eHMI with a participant study. The article elucidates underlying considerations and details of the test protocol that serves as framework to measure the behavior and subjective evaluations of non-automated road users when interacting with automated vehicles in an experimental setting. The standardized test procedure provides a useful framework for researchers and practitioners.

In this study, we investigate the environmental stability of the sulfide-based argyrodite solid electrolyte Li6PS5Cl0.5Br0.5, a promising candidate for all-solid-state lithium batteries due to its high ionic conductivity and favorable mechanical properties. Despite its potential, the material’s sensitivity to ambient air humidity presents challenges for large-scale battery manufacturing. Moisture exposure leads to performance degradation and the release of toxic hydrogen sulfide (H2S) gas, raising concerns for workplace safety. The objectives of this study are to validate the electrolyte synthesis process, evaluate the effects of air humidity exposure on its reactivity and ionic conductivity, and establish a standardized protocol for assessing environmental stability. We report a synthesis method based on ball milling and heat treatment that achieves an ionic conductivity of 2.11 mS/cm, along with a fundamental study incorporating modeling and formulation approaches to evaluate the electrolyte’s environmental stability. Furthermore, we introduce a simplified testing method for assessing environmental stability, which may serve as a benchmark protocol for the broader class of argyrodite solid electrolytes.

Monitoring position and movements of preterm infants is important to ensure their well-being and optimal development. This study evaluates the feasibility of a pressure-sensitive fiber-optic mattress (FM), made entirely of plastic, for two-dimensional analysis of preterm infant movements and positioning. Before clinical use, we developed a simple, replicable, and cost-effective test protocol to simulate infant movements and positions, enabling early identification of technical limitations. Using data from 20 preterm infants, we assessed the FM’s potential to monitor posture and limb motion. FM-derived pressure patterns were compared with camera-based manual annotations to distinguish between different positions and out-of-bed moments, as well as limb-specific movements. Bench-test results demonstrated the FM’s sensitivity to motion and pressure changes, supporting its use in preclinical validation. Clinical data confirmed the FM’s reliability in identifying infant positions and movement patterns, showing an accuracy comparable to camera annotations. However, limitations such as calibration, sensitivity to ambient light, and edge-related artifacts were noted, indicating areas for improvement. In conclusion, the test protocol proved effective for early-stage evaluation of smart mattress technologies. The FM showed promising clinical feasibility for non-obtrusive monitoring of preterm infants, though further optimization is needed for robust performance in neonatal care.

Background and aimsWalking is the core physical activity of older persons. The assessment of walking capacity is increasingly important for clinical purposes and clinical research. Differences between assessment tools and protocols for short walks to obtain gait characteristics can be responsible for changes, e.g., in gait speed from 0.1 to 0.2 m/s. The purpose of this study was to generate further knowledge for the harmonization and/or standardization of short walk-test protocols for assessing gait characteristics under supervised conditions.MethodsFor this cross-sectional study, 150 community-dwelling older adults (mean age 80.5 ± 4.5 years) were recruited. Participants performed eight walks differing in the distance (8-versus 4-m), static versus dynamic trials and comparing different test speed instructions (usual versus maximal) on an electronic walkway.ResultsA meaningful significant difference in mean usual gait speed was documented comparing the 4-m dynamic and static test protocol (0.12 m/s; p = 0.001). For the same comparison over an 8-m distance (dynamic versus static) and for the comparison between usual gait speed over 4-and 8-m, the differences in gait speed were smaller, but still statistically significant (p = 0.001).ConclusionsGait speed was faster, if the test protocol did not include a static start or stop. The differences were greater for a shorter walking distance. This aspect should be considered for the comparison of study results and is particularly relevant for systematic reviews and meta-analyses.

Contrary to the past in where polygraph examiners practiced an “intuitive-based practice” grounded on several industry leaders experience, consequently having various schools of thoughts, modern polygraph examiners follow a test protocol based and rooted in research. As effective as the “evidence- based- practice” is, it does not cover all ranges of cases, examinees, situations, and contaminations which may carry a misleading affect. The purpose of this paper is to draw examiners attention to the existing potential hazards surrounding a protocoled standard of practice and suggest solutions in order to alter the test to the examinee (“tailor made” style test) rather than alter the examinee to the test (“one size fits all” style test).

Human–Machine Interfaces (HMIs) in autonomous driving technology have recently gained significant research interest in public transportation. However, most of the studies are biased towards qualitative methods, while combining quantitative and qualitative approaches has yet to receive commensurate attention in measuring user acceptance of design outcome evaluation. To the best of our knowledge, no standardized test procedure that combines quantitative and qualitative methods has been formed to evaluate and compare the interrelationships between different designs of HMIs and their psychological effects on users. This paper proposes a practical and comprehensive protocol to guide assessments of user acceptance of HMI design solutions. We first defined user acceptance and analyzed the existing evaluation methods. Then, specific ergonomic factors and requirements that the designed output HMI should meet were identified. Based on this, we developed a protocol to evaluate a particular HMI solution from in- and out-of-vehicle perspectives. Our theoretical protocol combines objective and subjective measures to compare users’ behavior when interacting with Autonomous Vehicles (AVs) in a virtual experimental environment, especially in public transportation. Standardized testing procedures provide researchers and interaction designers with a practical framework and offer theoretical support for subsequent studies.

PurposeTo compare the effects of test protocols with different increments in workload and duration on peak oxygen uptake (\\[{\\dot{V}}\\]O2peak), and related physiological parameters during seated upper-body poling (UBP).MethodsThirteen upper-body trained, male individuals completed four UBP test protocols with increments in workload until volitional exhaustion in a counterbalanced order: 20 W increase/every 30 s, 20 W/60 s, 10 W/30 s and 10 W/60 s. Cardio-respiratory parameters and power output were measured throughout the duration of each test. Peak blood lactate concentration (bLapeak) was measured after each test.ResultsThe mixed model analysis revealed no overall effect of test protocol on \\[{\\dot{V}}\\]O2peak, peak minute ventilation (VEpeak), peak heart rate (HRpeak), bLapeak (all p ≥ 0.350), whereas an overall effect of test protocol was found on peak power output (POpeak) (p = 0.0001), respiratory exchange ratio (RER) (p = 0.024) and test duration (p < 0.001). There was no difference in POpeak between the 20 W/60 s (175 ± 25 W) and 10 W/30 s test (169 ± 27 W; p = 0.092), whereas POpeak was lower in the 10 W/60 s test (152 ± 21 W) and higher in the 20 W/30 s test (189 ± 30 W) compared to the other tests (all p = 0.001). In addition, RER was 9.9% higher in the 20 W/30 s compared to the 10 W/60 s test protocol (p = 0.003).ConclusionsThe UBP test protocols with different increments in workload and duration did not influence \\[{\\dot{V}}\\]O2peak, and can therefore be used interchangeably when \\[{\\dot{V}}\\]O2peak is the primary outcome. However, POpeak and RER depend upon the test protocol applied and the UBP test protocols can, therefore, not be used interchangeably when the latter is the primary outcome parameter.