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"wipe sampling"
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Evaluation of long-term data on surface contamination by antineoplastic drugs in pharmacies
PurposeThe handling of antineoplastic drugs represents an occupational health risk for employees in pharmacies. To minimize exposure and to evaluate cleaning efficacy, wipe sampling was used to analyze antineoplastic drugs on surfaces. In 2009, guidance values were suggested to facilitate the interpretation of results, leading to a decrease in surface contamination. The goal of this follow-up was to evaluate the time trend of surface contamination, to identify critical antineoplastic drugs and sampling locations and to reassess guidance values.MethodsPlatinum, 5-fluorouracil, cyclophosphamide, ifosfamide, gemcitabine, methotrexate, docetaxel and paclitaxel were analyzed in more than 17,000 wipe samples from 2000 to 2021. Statistical analysis was performed to describe and interpret the data.ResultsSurface contaminations were generally relatively low. The median concentration for most antineoplastic drugs was below the limit of detection except for platinum (0.3 pg/cm2). Only platinum and 5-fluorouracil showed decreasing levels over time. Most exceedances of guidance values were observed for platinum (26.9%), cyclophosphamide (18.5%) and gemcitabine (16.6%). The most affected wipe sampling locations were isolators (24.4%), storage areas (17.6%) and laminar flow hoods (16.6%). However, areas with no direct contact to antineoplastic drugs were also frequently contaminated (8.9%).ConclusionOverall, the surface contaminations with antineoplastic drugs continue to decrease or were generally at a low level. Therefore, we adjusted guidance values according to the available data. The identification of critical sampling locations may help pharmacies to further improve cleaning procedure and reduce the risk of occupational exposure to antineoplastic drugs.
Journal Article
PREHISTORIC CHARCOAL GRAFFITI DISCOVERED IN KATEŘINSKÁ CAVE, CZECH REPUBLIC
The article reports on a new sampling method and the archaeological context of cave drawings, which include the oldest currently known graffiti in the Czech Republic. Between 2016 and 2020, samples with a small amount of charcoal were taken from drawings found in Kateřinská Cave (Catherine’s Cave) of the Moravian Karst in the Czech Republic. A new gentle method of sampling charcoal from the cave walls was developed for the purpose of radiocarbon (14C) dating cave drawings of unknown age, while preserving the contours of the drawings. 14C analysis has provided data from four periods of prehistory and history: from the Neolithic around 5000 BC, the turn of the Neolithic and Eneolithic around 4300 BC, the Hallstatt Period from 800–450 BC, and also from the Middle Ages (13th century). The radiocarbon dates of the graffiti correspond to the dates of the pottery finds from the entrance portal of Kateřinská Cave, thus validating the dates and the sampling method.
Journal Article
An Improved LC–MS/MS Method for the Analysis of Thirteen Cytostatics on Workplace Surfaces
Cytostatics are drugs used in cancer treatment, which pose serious risks to healthcare workers. Dermal absorption via surface contamination is the key exposure route; thus, rapid, reliable, and validated analytical methods for multicomponent detection are crucial to identify the exposure risk. A surface-wipe-sampling technique compatible with hospitals’ safety requirements (gauze, 1 mL isopropanol) and a fast and simple extraction method (1 mL acetonitrile, 20 min ultrasonic bath, evaporation, reconstitution in 200 µL acetonitrile), coupled with liquid chromatography–tandem mass spectrometry analysis, were developed. It allowed identification and quantification of 13 cytostatics on surfaces: cyclophosphamide, doxorubicin, etoposide, ifosfamide, paclitaxel, bicalutamide, capecitabine, cyproterone, flutamide, imatinib, megestrol, mycophenolate mofetil, prednisone. Good linearity, sensitivity, and precision were achieved (R2 > 0.997, IDLs < 4.0 pg/cm2, average CV 16%, respectively). Accuracy for four model surfaces (melamine-coated wood, phenolic compact, steel 304, steel 316) was acceptable (80 ± 12%), except for capecitabine and doxorubicin. Global uncertainty is below 35% for concentrations above 100 pg/cm2 (except for capecitabine and doxorubicin)—a guidance value for relevant contamination. Method application in a Portuguese university hospital (28 samples) identified the presence of seven cytostatics, at concentrations below 100 pg/cm2, except for three samples. The widespread presence of cyclophosphamide evinces the necessity to review implemented procedures.
Journal Article
Skin, Clothing, and Surface Contamination with Metals at a Powder Additive Manufacturing Facility
Powder bed fusion (PBF) and directed energy deposition (DED) additive manufacturing use feedstock powders that contain metals associated with skin diseases. We performed a survey of surface contamination and limited task-based dermal exposure assessment (four employees) at a PBF and DED facility. Skin wipes of wrists for two employees in the PBF room had higher post-task levels of chromium, cobalt, molybdenum, and nickel. Personal clothing worn by PBF employees showed evidence of contamination with metals as did personal protective equipment (PPE). Microscopy analysis documented contamination of metals throughout most areas of the facility. Levels of metals on surfaces throughout the facility were (ng/cm2) <5.0–7247 (aluminum), <0.2–4899 (chromium),
Journal Article
Cytostatics in Indoor Environment: An Update of Analytical Methods
Periodic and adequate environmental monitoring programs are crucial to assess and reduce the occupational exposure of healthcare workers to cytostatics. The analytical methods employed should be rapid, reliable, sensitive, standardized, and include multiple compounds. A critical overview of recent overall procedures for surface and air contamination with cytostatics in workplace settings is presented, with a focus on sampling, sample preparation, and instrumental considerations. Limitations are also addressed and some recommendations and advice are provided. Since dermal absorption is the main exposure route, surface contamination is the preferred indicator of biological uptake and its methods have significantly improved. In contrast, cytostatics’ inhalation is rare; thus, air contamination has been poorly studied, with little improvement. Still, some elements of the analytical methods have not been extensively explored, namely: the amount of wetting solution, the extraction procedure, surface chemistry and roughness, recovery studies from specific surfaces, and cytostatics stability (in surfaces and during shipping and storage). Furthermore, complete validation data (including precision, accuracy, and instrumental and method detection limits) and estimation of global uncertainty are still lacking in most studies, thus preventing method comparison and proposal of standardized procedures.
Journal Article
High-Performance Anion Exchange Chromatography Electrochemical Determination of Uric Acid as a Contamination Marker
This study presents the first instance of determining environmental uric acid in urban dust using high-performance anion exchange chromatography coupled with electrochemical detection. The optimum chromatographic conditions were identified as a 10 mm × 4.6 mm, 10 µm anion exchange column with a mobile phase of pH 8 50 mM phosphate buffer. Cyclic voltametric investigations over a pH range of 2 to 12 showed that uric acid gave a single diffusion-controlled peak. Hydrodynamic voltametric studies of uric acid using a mobile phase of 50 mM pH 8.0 phosphate buffer over the range 0.0 V to +1.4 V (vs. stainless steel) showed a similar single oxidation wave, which plateaued at potentials more positive than +0.7 V (vs. stainless steel). An applied potential of +0.90 V (vs. stainless steel) was chosen for further investigations, and a linear range of 0.10 to 100 mg/L was obtained, with a detection limit of 0.866 mg/L based on a signal-to-noise ratio of 3. Dust wipe samples were extracted in pH 8, 50 mM phosphate buffer with the aid of sonication. Recoveries of 99.6% (% CV = 4.52%) were achieved for the dust wipe fortified with 16.8 µg of uric acid. Nitrate, nitrite, chloride, acetate, and sulfate ions were found not to interfere. The dust wipe samples were found to have uric acid levels of between 32.6 µg/m2 and 3.98 mg/m2.
Journal Article
Interception Systems in Assessment of Dermal Exposure to Pesticides: Laboratory Comparison of Media
Dermal exposure of workers to pesticides can be assessed using patches, placed on the workers’ clothes or used to wipe off the substance from the skin. Since there are no official indications of the materials to be used for patch sampling, a wide range of materials is suggested in the scientific literature. This paper reports a laboratory study on the affinity of four different pesticides widely used in southern Italy with five patch matrices. Imidacloprid, Hexythiazox, Boscalid and Myclobutanil were tested with cotton and gauze sheets, polyethylene tissue and two different grades of cellulose papers. An aerosol machine was used to nebulize the substance on the patches in a closed system, simulating the conditions of use on the workers’ clothes. The recovery of the analytes from the media was evaluated, by spiking the patches with a known amount of each active substance and testing their performances as skin wipes. Samples were extracted and analyzed in gas chromatography with an electron capture detector. The recovery from the spiked patches was 89–96% for all pesticides, while the test recoveries were very different. Results showed a higher affinity with Imidacloprid and Hexythiazox for gauze, with Myclobutanil for cotton and with Boscalid for paper filters (W41).
Journal Article
Dust: A Metric for Use in Residential and Building Exposure Assessment and Source Characterization
In this review, we examine house dust and residential soil and their use for identifying sources and the quantifying levels of toxicants for the estimation of exposure. We answer critical questions that focus on the selection of samples or sampling strategies for collection and discuss areas of uncertainty and gaps in knowledge. We discuss the evolution of dust sampling with a special emphasis on work conducted after the publication of the 1992 review by McArthur [Appl Occup Environ Hyg 7(9):599-606 (1992)]. The approaches to sampling dust examined include surface wipe sampling, vacuum sampling, and other sampling approaches, including attic sampling. The metrics of presentation of results for toxicants in dust surface loading (micrograms per square centimeter) or surface concentration (micrograms per gram) are discussed. We evaluate these metrics in terms of how the information can be used in source characterization and in exposure characterization. We discuss the types of companion information on source use and household or personal activity patterns required to assess the significance of the dust exposure. The status and needs for wipe samplers, surface samplers, and vacuum samplers are summarized with some discussion on the strengths and weaknesses of each type of sampler. We also discuss needs for research and development and the current status of standardization. Case studies are provided to illustrate the use of house dust and residential soil in source characterization, forensic analyses, or human exposure assessment.
Journal Article
Indoor Dust Metal Loadings: A Human Health Risk Assessment
In order to characterize the influence of environmental factors in dust metal loadings inside homes in an urban environment and to evaluate the associated potential health risks, samples of settled indoor dust from 10 apartments in the urban area of Madrid (Spain) were collected with wet wipes. Cd, Cr, Cu, Pb, Zn, Ni, and Mn loads were determined by Atomic Absorption Spectroscopy (AAS) after a HNO
3
+ H
2
O
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digestion. The environmental factors evaluated were load distribution between rooms, number of residents, presence of smokers, traffic intensity, apartment elevation, and frequency of house cleaning. Tukey’s range test and stepwise multiple linear regression analysis revealed that metal dust loadings present two prevailing origins: (1) They present higher loadings in the entry hall, which suggest that dust is tracked indoors adhered to footwear and clothing and (2) they arise from tobacco smoking. Significant correlations were also observed between metal loadings and traffic intensity (Cr), number of residents (Cr, Pb, and Cu), number of days between cleaning (Ni), and flat height (Mn). A human health risk assessment considering a mechanistic hand-to-mouth model for dust ingestion and dermal absorption revealed that urban children are not expected to develop adverse health effects from exposure to trace elements in household dust. The contribution of this exposure scenario to the overall received dose should be included when assessing the background exposure of children to trace elements. A more precise assessment should attempt to reduce the significant uncertainty of the risk model output associated with estimates of exposure variables, deposition rates, and metal bioaccessibility.
Journal Article
Expanding Antineoplastic Drugs Surface Monitoring Profiles: Enhancing of Zwitterionic Hydrophilic Interaction Methods
Antineoplastic drugs are a wide and heterogeneous group of substances that, as universally known, can cause highly severe toxic effects to whoever is exposed. From an occupational safety point of view, surface contaminations inside preparation and administration units are a growing issue and therefore require the development and implementation of sensible and fast monitoring methods. The unlikelihood of a unique all-embracing chromatography, able to correctly retain and separate each analyte led to the need to create an orthogonal normal phase analysis, which might be able to fill the gaps in the more common reversed-phase ones. An existing hydrophilic interaction method has thus been expanded to 6 other drugs and applied to real samples after an evaluation of its performances. The experimental data were then used to evaluate the possibility of estimating reliable relationships between the chromatographic retention and the chemical-structural features of the drugs under analysis.
Journal Article