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Development of a Multiplex PCR Method for the Detection of Six Common Foodborne Pathogens
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Development of a Multiplex PCR Method for the Detection of Six Common Foodborne Pathogens
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Development of a Multiplex PCR Method for the Detection of Six Common Foodborne Pathogens
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Journal Article
Development of a Multiplex PCR Method for the Detection of Six Common Foodborne Pathogens
2008
Overview
本研究開發了一種多重聚合酶連鎖反應(multiplex PCR)方法，用以篩選和檢測六株澳門常見的食源性致病菌。此方法使用六對引子對，可自混有數株參考菌株之食物樣本或單一菌種之純培養中，擴增產生特定大小之複製子(amplicon)。多重聚合酶連鎖反應方法所檢測的六個目標基因包括：大腸桿菌O157:H7之verocytotoxin (stx)基因、李斯特菌之hemolysin (hly)基因、沙門氏桿菌之invasion (invA)基因、霍亂弧菌之cholera toxin (ctx)基因、副溶血性弧菌之thermolabile hemolysin (tlh)基因，以及金黃色葡萄球菌之thermostable nuclease (nuc)基因。此檢測法之靈敏度爲1~100 CFU/mL。此多重聚合酶連鎖反應方法可檢測下列三類食物所含之病菌：肉類及肉類產品中之沙門氏桿菌、李斯特菌和大腸桿菌O157:H7；海鮮及海鮮產品中之霍亂弧菌和副溶血性弧菌；即時食品中之金黃色葡萄球菌。綜上所述，本研究結果顯示，多重聚合酶連鎖反應爲一快速之食源性致病菌檢測方法，可用於例行監測食物之安全，或評估其風險。
Publisher
衛生福利部食品藥物管理署,Food and Drug Administration
Subject
