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Reply to Rattanapitoon et al., “Reconsidering the Toxoplasma gondii interactome: opportunities beyond crosslinking mass spectrometry”
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Reply to Rattanapitoon et al., “Reconsidering the Toxoplasma gondii interactome: opportunities beyond crosslinking mass spectrometry”
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Journal Article
Reply to Rattanapitoon et al., “Reconsidering the Toxoplasma gondii interactome: opportunities beyond crosslinking mass spectrometry”
2025
Overview
REPLY We thank Rattanapitoon and colleagues for their thoughtful comments on our study integrating crosslinking mass spectrometry (XL-MS) with machine learning to map a Toxoplasma gondii interactome. PROTEOMIC “VISIBILITY” AND STAGE-RESTRICTED BIOLOGY We agree that our cytosolic preparation—performed without additional subcellular fractionation—prioritized abundant complexes (e.g., ribosome, proteasome) and that this limits sensitivity to low-abundance or stage-restricted proteins. Mapping a Toxoplasma gondii interactome by crosslinking mass spectrometry and machine learning. mBio 16:e02159-25.
Publisher
American Society for Microbiology
Subject
