Journal Article

Accounting for cellular-level variation in lysis: implications for virus–host dynamics

Dominguez-Mirazo, Marian, Harris, Jeremy D., Demory, David, Weitz, Joshua S.

2024

Overview

Quantifying viral traits—including the adsorption rate, burst size, and latent period—is critical to characterize viral infection dynamics and develop predictive models of viral impacts across scales from cells to ecosystems. Here, we revisit the gold standard of viral trait estimation—the one-step growth curve—to assess the extent to which assumptions at the core of viral infection dynamics lead to ongoing and systematic biases in inferences of viral traits. We show that latent period estimates obtained via one-step growth curves systematically underestimate the mean latent period and, in turn, overestimate the rate of viral killing at population scales. By explicitly incorporating trait variability into a dynamical inference framework that leverages both virus and host time series, we provide a practical route to improve estimates of the mean and variance of viral traits across diverse virus–microbe systems.

• Publisher: American Society for Microbiology
• Subject: Adsorption / bacteriophage lysis / Bacteriophages / Bacteriophages - genetics / Bacteriophages - growth & development / Bacteriophages - physiology / Burst size / cellular variability / Computer applications / Ecology, environment / Estimates / Growth curves / Host Microbial Interactions / Host-Pathogen Interactions / inference / Latent period / Life Sciences / Lysis / mathematical modeling / Microbiology and Parasitology / Models, Biological / Nonlinear Dynamics / phage ecology / Phages / Phenotypes / Physiology / population dynamics / Population studies / Prediction models / Research Article / Simulation / Symbiosis / Viral infections / Viruses