Asset Details
Do you wish to reserve the book?
A red fluorescent protein with improved monomericity enables ratiometric voltage imaging with ASAP3
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
A red fluorescent protein with improved monomericity enables ratiometric voltage imaging with ASAP3
Do you wish to request the book?
A red fluorescent protein with improved monomericity enables ratiometric voltage imaging with ASAP3
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Paper
A red fluorescent protein with improved monomericity enables ratiometric voltage imaging with ASAP3
2020
Overview
Abstract A ratiometric genetically encoded voltage indicator (GEVI) would be desirable for tracking transmembrane voltage changes in cells that are undergoing motion. To create a high-performance ratiometric GEVI, we explored the possibility of adding a voltage-independent red fluorophore to ASAP3, a high-gain green fluorescent GEVI. We performed combinatorial multi-site mutagenesis on the cyan-excitable red fluorescent protein mCyRFP1 to enhance brightness and monomericity, creating mCyRFP3. Among red fluorescent proteins tested, mCyRFP3 proved to be the least perturbing when fused to ASAP3. We demonstrate that the red fluorescence of ASAP3-mCyRFP3 (ASAP3-R3) provides an effective reference channel to remove motion artifacts from voltage-induced changes in green fluorescence. Finally we use ASAP3-R3 to visualize membrane voltage changes throughout the cell cycle of motile cells. Competing Interest Statement The authors have declared no competing interest. Footnotes * mCyRFP2 name changed to mCyRFP3, as a different mCyRFP2 was recently published
Publisher
Cold Spring Harbor Laboratory Press,Cold Spring Harbor Laboratory
Subject
