Paper

Resource reallocation in engineered Escherichia coli strains with reduced genomes

Muñoz, Nathalie, Kumar, Neeraj, Francis, Ryan M, Zucker, Jeremy D, Nicora, Carrie D, Cottam, Joseph A, Egbert, Robert G, Mitchell, Hugh D, Young-Mo, Kim, Chazin-Gray, Adam M, Nakayasu, Ernesto S, Auberry, Deanna L, Nelson, William C, Eaton, Arielle M

2020

Overview

Abstract A major challenge in synthetic biology is properly balancing evolved and engineered functions without compromising microbial fitness. Many microbial proteins are not required for growth in regular laboratory conditions, but it is unclear what fraction of the proteome can be eliminated to increase bioproduction and maintain fitness. Here, we investigated the effects of massive genome reduction in E. coli on the expression level and evolutionary stability of a model biosynthetic pathway to produce the pigment protodeoxyviolacein (PDV). We identified an amino acid metabolism imbalance and compromised growth that were correlated with elimination of genes associated with significant proteome fraction. Proteomic profiling suggested that increased amino acid pools are responsible for an alleviation of fitness defects associated with PDV expression. In addition, all strains with genome reductions that significantly affected the proteome exhibited decreased stability of PDV production compared to the wild-type strain under persistent PDV expression conditions despite the alleviation of fitness defects. These findings exhibit the importance of balancing evolved functions with engineered ones to achieve an optimal balance of fitness and bioproduction. Competing Interest Statement The authors have declared no competing interest.

• Publisher: Cold Spring Harbor Laboratory Press,Cold Spring Harbor Laboratory
• Subject: Amino acids / Bioengineering / Genomes / Proteomes / Reproductive fitness