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Human neutrophils communicate remotely via glutamate-induced glutamate release
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Human neutrophils communicate remotely via glutamate-induced glutamate release
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Human neutrophils communicate remotely via glutamate-induced glutamate release
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Paper
Human neutrophils communicate remotely via glutamate-induced glutamate release
2023
Overview
Neutrophils are white blood cells that are critical to the acute inflammatory and adaptive immune responses. Their swarming-pattern behaviour is controlled by multiple cellular cascades involving calcium-dependent release of various signalling molecules. Previous studies have reported that neutrophils express glutamate receptors and can release glutamate but evidence of direct neutrophil-neutrophil communication has been elusive. Here, we hold semi-suspended cultured human neutrophils in patch-clamp whole-cell mode to find that calcium mobilisation induced by stimulating one neutrophil can trigger an NMDA receptor-driven membrane current and calcium signal in neighbouring neutrophils. We employ an enzymatic-based imaging assay to image, in real time, glutamate release from neutrophils induced by glutamate released from their neighbours. These observations provide direct evidence for a positive-feedback inter-neutrophil communication that could contribute to mechanisms regulating communal neutrophil behaviour.Competing Interest StatementThe authors have declared no competing interest.Footnotes* This version has updated data selection and analyses, focusing on a shorter time span after cell isolation and suspension.
Publisher
Cold Spring Harbor Laboratory Press,Cold Spring Harbor Laboratory
