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Single-cell characterization of neovascularization using hiPSC-derived endothelial cells in a 3D microenvironment
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Single-cell characterization of neovascularization using hiPSC-derived endothelial cells in a 3D microenvironment
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Paper
Single-cell characterization of neovascularization using hiPSC-derived endothelial cells in a 3D microenvironment
2022
Overview
The formation of vascular structures is fundamental for in vitro tissue engineering. Vascularization can enable the nutrient supply within larger structures and increase transplantation efficiency, which are currently limiting factors in organoid research. We differentiated human induced pluripotent stem cells toward endothelial cells in 3D suspension culture. To investigate in vitro neovascularization and various 3D microenvironmental approaches, we designed a comprehensive single-cell transcriptomic study. Time-resolved single-cell transcriptomics of the endothelial and co-evolving mural cells gave insights into cell type development, stability, and plasticity. Transfer to a 3D hydrogel microenvironment induced neovascularization and facilitated tracing of sprouting, coalescing, and tubulogenic endothelial cells states. During maturation, we monitored two pericyte subtypes evolving of mural cells. Profiling cell-cell interactions between pericytes and endothelial cells confirmed in vivo angiogenic signaling and emphasized new cytokine signals during tubulogenesis. Our data, analyses, and results provide an in vitro roadmap to guide vascularization in future tissue engineering. Competing Interest Statement The authors have declared no competing interest.
Publisher
Cold Spring Harbor Laboratory Press,Cold Spring Harbor Laboratory
Subject
