Asset Details
Do you wish to reserve the book?
P590 Vaginal microbiota and douching cessation: a crossover pilot study
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
P590 Vaginal microbiota and douching cessation: a crossover pilot study
Do you wish to request the book?
P590 Vaginal microbiota and douching cessation: a crossover pilot study
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
P590 Vaginal microbiota and douching cessation: a crossover pilot study
2019
Overview
BackgroundObservational studies have demonstrated a dose-dependent association between vaginal douching and bacterial vaginosis. We sought to estimate the effect of douching cessation on the vaginal microbiota in a pilot crossover study.MethodsThirty-two women self-collected vaginal swabs twice-weekly (n=950) during a douching observational phase (“D”, 4 weeks), followed by douching cessation (“DC”, 12 weeks). Vaginal microbiota were characterized by 16S rRNA gene sequencing (V3-V4) and clustered into community state types (CSTs). A conditional logistic regression model, adjusted for menstruation and sexual behaviors, allowed each woman to serve as her own control. Wilcoxon signed-rank tests were used to evaluate paired changes in microbiota between phases. Broad-range qPCR assays provided estimates of bacterial absolute abundance per swab. A piecewise linear mixed effects model was used to assess differences in rates of change in bacterial absolute abundance before and after douching.ResultsThere was not a statistically significant change in the odds of Lactobacillus-dominated CSTs comparing DC to D (aOR 0.54, 95% CI: 0.27–1.11). There were no significant changes for four individual Lactobacillus spp. and no meaningful changes in other taxa investigated. The rates of change in bacterial absolute abundance was not significantly different in samples collected 3 days before and after douching (p=0.46). Women who had a Lactobacillus-dominated CST at baseline experienced shifts to low-Lactobacillus CST in DC, and vice versa for women who had a low-Lactobacillus CST at baseline (interaction on entry CST, p-value <0.02), however, these findings were driven by changes occurring in the final weeks.ConclusionIn this pilot study, douching cessation was not associated with major changes in vaginal microbiota. Shifts in Lactobacillus-dominance may represent regression to the mean as the shifts occurred late in DC, giving ample time for fluctuations. Disparate findings between this study and prior analyses using Nugent score may be related to low-Lactobacillus CSTs receiving low/intermediate Nugent scores.DisclosureNo significant relationships.
Publisher
BMJ Publishing Group LTD
Subject
