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New Insights into Lung Surfactant Monolayers Using Vibrational Sum Frequency Generation Spectroscopy
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New Insights into Lung Surfactant Monolayers Using Vibrational Sum Frequency Generation Spectroscopy
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New Insights into Lung Surfactant Monolayers Using Vibrational Sum Frequency Generation Spectroscopy
New Insights into Lung Surfactant Monolayers Using Vibrational Sum Frequency Generation Spectroscopy
Journal Article

New Insights into Lung Surfactant Monolayers Using Vibrational Sum Frequency Generation Spectroscopy

2006
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Overview
At the air–water interface, interfacial molecular structure, intermolecular interactions, film relaxation and film respreading of model lung surfactant monolayers were studied using vibrational sum frequency generation (VSFG) spectroscopy combined with a Langmuir film balance. Chain-perdeuterated dipalmitoylphosphatidylcholine (DPPC-d62), palmitoyloleoylphosphatidylglycerol (POPG), palmitic acid (PA) and tripalmitin were investigated. In the DPPC-d62–PA binary monolayer, PA showed a condensing effect on the DPPC chains. On the contrary, in the DPPC-d62–POPG binary monolayer, POPG showed a fluidizing effect on the DPPC chains. In the ternary monolayer system of DPPC-d62–POPG–PA, the balance between the fluidizing and the condensing effect was also observed. In addition, the film relaxation behavior of DPPC-d62 and the enhanced film stability of DPPC-d62 caused by the addition of tripalmitin were observed. Real-time VSFG was also employed to study the respreading properties of a complex lung surfactant mixture containing DPPC-d62, POPG, PA and KL4 (a mimic of SP-B) peptide, which revealed DPPC enrichment after film compression.