Journal Article

Atypical initial cleavage patterns minimally impact rhesus macaque in vitro embryo morphokinetics and embryo outgrowth development

Golos, Thaddeus G., Schmidt, Jenna Kropp, Mean, Katherine D., Makulec, Allison T., Hinkle, Hayly M., Jones, Kathryn M., Block, Lindsey N., Bowman, Brittany D.

2023

Overview

Embryo morphokinetic analysis through time-lapse embryo imaging is envisioned as a method to improve selection of developmentally competent embryos. Morphokinetic analysis could be utilized to evaluate the effects of experimental manipulation on pre-implantation embryo development. The objectives of this study were to establish a normative morphokinetic database for in vitro fertilized rhesus macaque embryos and to assess the impact of atypical initial cleavage patterns on subsequent embryo development and formation of embryo outgrowths. The cleavage pattern and the timing of embryo developmental events were annotated retrospectively for unmanipulated in vitro fertilized rhesus macaque blastocysts produced over four breeding seasons. Approximately 50% of the blastocysts analyzed had an abnormal early cleavage event. The time to the initiation of embryo compaction and the time to completion of hatching was significantly delayed in blastocysts with an abnormal early cleavage event compared to blastocysts that had cleaved normally. Embryo hatching, attachment to an extracellular matrix, and growth during the implantation stage in vitro was not impacted by the initial cleavage pattern. These data establish normative morphokinetic parameters for in vitro fertilized rhesus macaque embryos and suggest that cleavage anomalies may not impact embryo implantation rates following embryo transfer. Summary Sentence Cleavage dimorphisms are prevalent in in vitro fertilized rhesus macaque embryos and these early cleavage anomalies minimally impact embryo morphokinetics and the events of implantation in vitro. Graphical Abstract

• Publisher: Society for the Study of Reproduction,Oxford University Press
• Subject: Animals / Blastocyst / Blastocysts / Breeding seasons / cleavage / Embryo Culture Techniques - methods / Embryo Culture Techniques - veterinary / embryo development / Embryo Implantation / Embryo transfer / Embryo, Mammalian / Embryonic Development / Embryos / Extracellular matrix / Fertilization in Vitro - methods / Fertilization in Vitro - veterinary / Hatching / Macaca mulatta / macaque / morphokinetics / RESEARCH ARTICLE / Retrospective Studies / Time-Lapse Imaging - methods