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Molecular Characterizations of Avian Polyomavirus Isolated from Budgerigar in China
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Molecular Characterizations of Avian Polyomavirus Isolated from Budgerigar in China
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Journal Article
Molecular Characterizations of Avian Polyomavirus Isolated from Budgerigar in China
2008
Overview
Budgerigar fledgling disease is an acute viral infectious disease caused by avian polyomavirus (APV). In this study, 34 liver tissue samples of young, dead budgerigar with typical symptoms were collected in 2004. All the samples had positive polymerase chain reaction (PCR) test based on the VP1 specific primers. VP1 genes of these samples were sequenced and had high similarities to each other (99%–100%). A strain (HBYM02) was isolated and sequenced. As shown in the phylogenetic tree, there are two branches. One branch was composed by strains isolated from Passeriformes, and the other was composed only by one strain isolated from Falconiformes. The genome similarities between our isolate and other reported isolates were very high (>99%), and the evolution distances in the phylogenetic tree were very short (<0.005), which suggests that APV in China has the same genotype as those in other regions. The results will be useful for the diagnoses of, and vaccine development for, APV. Abbreviations: APV = avian polyomavirus; BFD = budgerigar fledgling disease; CEF = chicken embryo fibroblast; ORF = open reading frame; PCR = polymerase chain reaction; VP = viral protein Caracterización molecular de un poliomavirus aviar aislado de pericos Australianos en China. La enfermedad de polluelos de perico es una enfermedad viral aguda causada por un poliomavirus aviar. En el presente estudio, 34 muestras de tejido hepático provenientes de pericos Australianos jóvenes muertos con signos típicos de la enfermedad se colectaron durante el año 2004. Todas las muestras resultaron positivas a la prueba de reacción en cadena por la polimerasa basada en iniciadores específicos para la proteína viral 1. Se secuenciaron los genes de la proteína viral 1 de estas muestras mostrando altas similitudes entre sí (99 a 100 %). Se aisló y secuenció una cepa identificada como HBYMO2. Como se muestra en el árbol filogenético, existen dos ramas. Una rama compuesta por cepas aisladas de paseriformes y la otra compuesta por una cepa aislada de falconiformes. Las similitudes genéticas entre nuestro aislamiento y otros aislamientos reportados fue muy alta (>99%) y las distancias evolutivas en el árbol filogenético muy corta (<0.005), lo que sugiere que los poliomavirus de China tienen el mismo genotipo que los de otras regiones. Estos resultados serán útiles para el diagnóstico y desarrollo de vacunas contra poliomavirus aviar.
Publisher
American Association of Avian Pathologists
Subject
