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Preparation and physical properties of a novel biocompatible porcine corneal acellularized matrix

by Zhou, Yueping , Quyang, Luowa , Shao, Yi , Tang, Jing , Tan, Yuehui , Yin, Tingting , Qiu, Fangfang , Liu, Zuguo , Lin, Zhirong , Liu, Qiuping

in Animal Genetics and Genomics / Animals / Apoptosis / Biocompatibility / Biocompatible Materials - metabolism / Biodegradation / Biomaterials / Biomedical and Life Sciences / BIOTECHNOLOGY / Cell Biology / Cell Culture / Cell Proliferation / Cells, Cultured / Cornea / Cornea - cytology / Cornea - metabolism / Corneal Stroma - cytology / Cytotoxicity / Developmental Biology / Epithelial cells / Epithelium / Epithelium, Corneal - cytology / Extracellular Matrix - metabolism / Heterologous transplantation / Histology / Humans / Life Sciences / Materials Testing / Ophthalmology / Physical properties / Porosity / Scaffolds / Sincalide - metabolism / Software / Stability / Statistical analysis / Stem Cells / Sus scrofa / swine / Tissue engineering / Tissue Engineering - methods / Tissue grafting / Variance analysis / Water - metabolism / Water uptake

2010

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Preparation and physical properties of a novel biocompatible porcine corneal acellularized matrix

by Zhou, Yueping , Quyang, Luowa , Shao, Yi , Tang, Jing , Tan, Yuehui , Yin, Tingting , Qiu, Fangfang , Liu, Zuguo , Lin, Zhirong , Liu, Qiuping

2010

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Preparation and physical properties of a novel biocompatible porcine corneal acellularized matrix

by Zhou, Yueping , Quyang, Luowa , Shao, Yi , Tang, Jing , Tan, Yuehui , Yin, Tingting , Qiu, Fangfang , Liu, Zuguo , Lin, Zhirong , Liu, Qiuping

2010

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Journal Article

Preparation and physical properties of a novel biocompatible porcine corneal acellularized matrix

Zhou, Yueping,

Quyang, Luowa,

Shao, Yi,

Tang, Jing,

Tan, Yuehui,

Yin, Tingting,

Qiu, Fangfang,

Liu, Zuguo,

Lin, Zhirong,

Liu, Qiuping

2010

Overview

This study was to investigate the stability, physico-mechanical property and biocompatibility of porcine corneal acellularized matrix (PCAM) that was prepared using human sera treatment to decellularize corneas. The stability (the rate of biodegradation) and physico-mechanical property (water uptake, density, and porosity) of PCAM were not compromised, compared with porcine fresh cornea matrix (PFCM, p > 0.05). The contact and extract cytotoxicity tests with human corneal epithelial cells and human keratocytes showed that PCAM has a good biocompatibility ex vivo and no cytotoxic effect. These results present the ability to create safety scaffolds that function as cornea grafts and provide a novel experimental approach for the study of cornea tissue engineering using acellular porcine cornea.

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Publisher

New York : Springer-Verlag,Springer Science + Business Media,Springer-Verlag,Society for In Vitro Biology

Subject

Animal Genetics and Genomics

/ Animals

/ Apoptosis

/ Biocompatibility

/ Biocompatible Materials - metabolism

/ Biodegradation

/ Biomaterials