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Mass spectrometry-based metabolomics of single yeast cells
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Mass spectrometry-based metabolomics of single yeast cells
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Journal Article
Mass spectrometry-based metabolomics of single yeast cells
2013
Overview
Single-cell level measurements are necessary to characterize the intrinsic biological variability in a population of cells. In this study, we demonstrate that, with the microarrays for mass spectrometry platform, we are able to observe this variability. We monitor environmentally (2-deoxy- d -glucose) and genetically (ΔPFK2) perturbed Saccharomyces cerevisiae cells at the single-cell, few-cell, and population levels. Correlation plots between metabolites from the glycolytic pathway, as well as with the observed ATP/ADP ratio as a measure of cellular energy charge, give biological insight that is not accessible from population-level metabolomic data.
Publisher
National Academy of Sciences,National Acad Sciences
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