Journal Article

Freeze-Dried Human Platelet-Rich Plasma Retains Activation and Growth Factor Expression after an Eight-Week Preservation Period

Sainoh, Takeshi, Aoki, Yasuchika, Ohtori, Seiji, Yamashita, Masaomi, Kanamoto, Hirohito, Ito, Michihiro, Koda, Masao, Shiga, Yasuhiro, Toyone, Tomoaki, Orita, Sumihisa, Fujimoto, Kazuki, Inage, Kazuhide, Iseki, Toru, Furuya, Takeo, Takahashi, Kazuhisa, Inoue, Masahiro, Kubota, Go, Eguchi, Yawara, Kinoshita, Hideyuki, Yamauchi, Kazuyo, Sato, Jun, Kamoda, Hiroto, Abe, Koki

2017

Overview

Study DesignControlled laboratory study.PurposeThis study aimed to evaluate the efficacy of platelet-rich plasma (PRP) stored at room temperature (RT), frozen, or after freeze-drying.Overview of LiteraturePRP enriches tissue repair and regeneration, and is a novel treatment option for musculoskeletal pathologies. However, whether biological activity is preserved during PRP storage remains uncertain.MethodsPRP was prepared from blood of 12 healthy human volunteers (200 mL/person) and stored using three methods: PRP was stored at RT with shaking, PRP was frozen and stored at −80℃, or PRP was freeze-dried and stored at RT. Platelet counts and growth factor content were examined immediately after preparation, as well as 2, 4, and 8 weeks after storage. Platelet activation rate was quantified by flow cytometry.ResultsPlatelet counts were impossible to determine in many RT samples after 2 weeks, but they remained at constant levels in frozen and freeze-dried samples, even after 8 weeks of storage. Flow cytometry showed approximately 80% activation of the platelets regardless of storage conditions. Almost no growth factors were detected in the RT samples after 8 weeks, while low but significant expression was detected in the frozen and freeze-dried PRP. Over time, the mean relative concentrations of various growth factors decreased significantly or disappeared in the RT group. In the frozen group, levels were maintained for 4 weeks, but decreased significantly by 8 weeks (p <0.05). The freeze-dried group maintained baseline levels of growth factors for the entire 8-week duration.ConclusionsFreeze-drying enables PRP storage while maintaining bioactivity and efficacy for extended periods.

• Publisher: Korean Society of Spine Surgery,Korean Spine Society,대한척추외과학회
• Subject: Antibodies / Basic Study / Biological activity / Blood platelets / Blood preservation / Epidermal growth factor / Flow cytometry / Freeze drying / Growth factors / Plasma / Platelet-rich plasma / Platelets / Storage conditions / Surgery / Vascular endothelial growth factor / 정형외과학