Journal Article

IDDF2022-ABS-0109 Clinical validation of a microbial stool test: towards a quantitative determination of intestinal dysbiosis

Galindo, Lia Oliver, Pagès, Mariona Serra, Oduber, Sara, Rodríguez, Marta Malagón, Manté, Xavier Aldeguer, Pujol, Sara Ramió, Lacosta, Eva

2022

Overview

BackgroundMany studies have shown the importance of the relationship between intestinal microbiota and health in recent years. This has led to the development of different microbiota tests, but still, there is a need to provide tests that determine dysbiosis. In this line, it was developed a microbial panel to summarize the gut microbiota state and identify dysbiosis, consisting of 15 key microbial markers representing the human intestinal microbiota.This work aimed to clinically validate the robustness of the microbial markers panel by comparing its outcomes with clinical parameters and the dysbiosis index use.MethodsThere were recruited 154 subjects undergoing the microbiota panel analysis. Clinical data and diagnosis of the enrolled subjects were recorded. Total DNA was extracted, and the abundance of the microbial markers was analysed by qPCR. Statistical analysis was performed using RStudio.ResultsA MANOVA analysis determined no significant differences between genders when the microbial abundance that composes the panel is analysed. In addition, no significant differences were observed according to the patient‘s age classification. The microbial panel showed significant differences (p-value 0.017) according to the type of stool (diarrhoea, constipation, or mixed), observing an inverse behaviour in A.muciniphila and M.smithii. The dysbiosis index was divided into healthy, temporary, and dysbiotic. Significant differences were observed when comparing the abundance of the markers to the dysbiosis index (p-value <0.001), showing how when the dysbiosis index is healthy; there is minimal variation in the microbial markers panel; when it is temporary, the variation increases, and when it is dysbiotic, the general dysbiosis is evident.ConclusionsThe clinical validation of the panel of microbial markers demonstrated to be a good indicator of the general state of the intestinal microbiota and to overcome 2 factors: gender and age, and to differentiate 2 dysbiosis degrees.This test could be a potential first step towards the quantification of the intestinal microbiota not only with application to digestive diseases but for all conditions that have already been shown to have dysbiosis.

• Publisher: BMJ Publishing Group Ltd and British Society of Gastroenterology,BMJ Publishing Group LTD
• Subject: Abundance / Basic Gastroenterology / Constipation / Diarrhea / Digestive system diseases / Dysbacteriosis / Intestinal microflora / Intestine / Microbiota / Statistical analysis