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Genetically encoded multimeric tags for intracellular protein localisation in cryo-EM
Genetically encoded multimeric tags for intracellular protein localisation in cryo-EM
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Genetically encoded multimeric tags for intracellular protein localisation in cryo-EM
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Genetically encoded multimeric tags for intracellular protein localisation in cryo-EM
Genetically encoded multimeric tags for intracellular protein localisation in cryo-EM
Paper

Genetically encoded multimeric tags for intracellular protein localisation in cryo-EM

2022
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Overview
Cryo-electron tomography is a powerful label-free tool for visualizing biomolecules in their native cellular context at molecular resolution. However, the precise localisation of biomolecules of interest in the tomographic volumes is challenging. Here, we present a tagging strategy for intracellular protein localisation based on genetically encoded multimeric particles (GEMs). We show the applicability of drug-controlled GEM labelling of endogenous proteins in cryo-electron tomography and cryo-correlative fluorescence imaging in human cells.
Publisher
Cold Spring Harbor Laboratory
Subject

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