Paper

REV-ERBα mediates complement expression and circadian regulation of microglial synaptic phagocytosis

Guo, Chun, Sheehan, Patrick W., Dimitry, Julie M., Lee, Jiyeon, Kanan, Michael F., Griffin, Percy, Musiek, Erik S., Zhang, Jinsong

2020

Overview

The circadian clock has been shown to regulate various aspects of brain health including microglial and astrocyte activation. Here we report that deletion of the master clock protein BMAL1 induces robust increases in the expression of complement genes such as C3, C4b and C1q in the hippocampus. Loss of downstream REV-ERBα-mediated transcriptional repression led to increases in C4b in neurons and astrocytes as well as C3 protein in microglia and astrocytes. REV-ERBα deletion induced complement C3/C4b gene expression and increased microglial phagocytosis of synapses in the CA3 region of the hippocampus. Finally, we observed diurnal variation in the degree of microglial synaptic phagocytosis in wild type mice which was abrogated by REV-ERBα deletion. This work uncovers the BMAL1-REV-ERBα axis as a regulator of complement expression and synaptic phagocytosis in the brain, thereby illuminating a novel mechanism of synaptic regulation by the circadian clock.

• Publisher: Cold Spring Harbor Laboratory
• Subject: Neuroscience