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Postmitotic Nuclear Pore Assembly Proceeds By Radial Dilation Of Small ER Membrane Openings
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Postmitotic Nuclear Pore Assembly Proceeds By Radial Dilation Of Small ER Membrane Openings
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Paper
Postmitotic Nuclear Pore Assembly Proceeds By Radial Dilation Of Small ER Membrane Openings
2017
Overview
The nuclear envelope has to be reformed after mitosis to create viable daughter cells with closed nuclei. How membrane sealing of DNA and assembly of nuclear pore complexes (NPCs) are achieved and coordinated is poorly understood. Here, we reconstructed nuclear membrane topology and structure of assembling NPCs in a correlative three dimensional electron microscopy time-course of dividing human cells. Our quantitative ultrastructural analysis shows that nuclear membranes form from highly fenestrated ER sheets, whose shrinking holes are stabilized and then dilated into NPCs during inner ring complex assembly, forming thousands of transport channels within minutes. This mechanism is fundamentally different from interphase NPC assembly and explains how mitotic cells can rapidly establish a closed nuclear compartment while making it transport-competent at the same time.
Publisher
Cold Spring Harbor Laboratory Press,Cold Spring Harbor Laboratory
Subject
