Asset Details
Do you wish to reserve the book?
Differential Effects of SUMO1/2 on Circadian Protein PER2 Stability and Function
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Differential Effects of SUMO1/2 on Circadian Protein PER2 Stability and Function
Do you wish to request the book?
Differential Effects of SUMO1/2 on Circadian Protein PER2 Stability and Function
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Paper
Differential Effects of SUMO1/2 on Circadian Protein PER2 Stability and Function
2019,2020
Overview
Posttranslational modification (PTM) of core circadian clock proteins, including Period2 (PER2), is required for proper circadian regulation. PER2 function is regulated by casein kinase 1 (CK1)-mediated phosphorylation and ubiquitination but little is known about other PER2 PTMs. We found that PER2 can be SUMOylated by both SUMO1 and SUMO2; however, SUMO1 versus SUMO2 conjugation had different effects on PER2 turnover and subcellular localization. SUMO2 conjugation facilitated PER2 degradation. In contrast, SUMO1 conjugation, mediated by E3 SUMO-protein ligase RanBP2, enhanced CK1-mediated PER2S662 phosphorylation and increased PER2 nuclear accumulation, chromatin association and transcriptional suppressor function. PER2 K736 was critical for both SUMO1- and SUMO2-conjugation. A PER2K736R mutation was sufficient to alter circadian periodicity, decrease PER2 nuclear entry and abolish PER2-mediated transcriptional suppression. Together, our data revealed differential effects of SUMO1 versus SUMO2 conjugation on PER2 function and demonstrated the effect of PER2 SUMOylation on the circadian regulatory system and circadian periodicity. Footnotes * Figure 1, Figure 2 and Supplemental files updated
Publisher
Cold Spring Harbor Laboratory Press,Cold Spring Harbor Laboratory
