Asset Details
Do you wish to reserve the book?
Exploring genetic interaction manifolds constructed from rich phenotypes
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Exploring genetic interaction manifolds constructed from rich phenotypes
Do you wish to request the book?
Exploring genetic interaction manifolds constructed from rich phenotypes
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Paper
Exploring genetic interaction manifolds constructed from rich phenotypes
2019
Overview
Synergistic interactions between gene functions drive cellular complexity. However, the combinatorial explosion of possible genetic interactions (GIs) has necessitated the use of scalar interaction readouts (e.g. growth) that conflate diverse outcomes. Here we present an analytical framework for interpreting manifolds constructed from high-dimensional interaction phenotypes. We applied this framework to rich phenotypes obtained by Perturb-seq (single-cell RNA-seq pooled CRISPR screens) profiling of strong GIs mined from a growth-based, gain-of-function GI map. Exploration of this manifold enabled ordering of regulatory pathways, principled classification of GIs (e.g. identifying true suppressors), and mechanistic elucidation of synthetic lethal interactions, including an unexpected synergy between CBL and CNN1 driving erythroid differentiation. Finally, we apply recommender system machine learning to predict interactions, facilitating exploration of vastly larger GI manifolds.
Publisher
Cold Spring Harbor Laboratory Press,Cold Spring Harbor Laboratory
Subject
