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Fatty acid biosynthesis redirected to medium chains in transgenic oilseed plants
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Fatty acid biosynthesis redirected to medium chains in transgenic oilseed plants
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Journal Article
Fatty acid biosynthesis redirected to medium chains in transgenic oilseed plants
1992
Overview
Medium-chain fatty acids (FAs), found in storage lipids of certain plants, are an important renewable resource. Seeds of undomesticated California bay accumulate laurate (12:0), and a 12:0-acyl-carrier protein thioesterase (BTE) has been purified from this tissue. Sequencing of BTE enabled the cloning of a complementary DNA coding for a plastid-targeted preprotein. Expression of the complementary DNA in the seeds of Arabidopsis thaliana resulted in BTE activity, and medium chains accumulated at the expense of long-chain (greater than or equal to 16) FAs. Laurate became the most abundant FA species and was deposited in the storage triacylglycerols. These results demonstrate a mechanism for medium-chain FA synthesis in plants
Publisher
American Society for the Advancement of Science,American Association for the Advancement of Science
Subject
/ Acetyltransferases - genetics
/ Acetyltransferases - metabolism
/ Acyl-Carrier Protein S-Acetyltransferase
/ acyl-carrier protein thioesterase
/ ADN
/ Agronomy. Soil science and plant productions
/ Biological and medical sciences
/ BRASSICA
/ cDNA
/ CLONAGE
/ CLONING
/ DNA
/ Fatty Acids - isolation & purification
/ FOOD
/ Fundamental and applied biological sciences. Psychology
/ Genetic engineering applications
/ genetically modified organisms
/ genetics
/ Genetics and breeding of economic plants
/ GRAINE
/ HERBS
/ Laurates
/ Lipids
/ Plant breeding: fundamental aspects and methodology
/ PLANTS
/ Plants, Genetically Modified
/ Plasmids
/ Plastids
/ seeds
/ SEMILLA
