Journal Article

Global Analysis of Protein Activities Using Proteome Chips

Bertone, Paul, Miller, Perry, Houfek, Thomas, Dean, Ralph A., Snyder, Michael, Lan, Ning, Zhu, Heng, Jansen, Ronald, Gerstein, Mark, Mitchell, Tom, Casamayor, Antonio, Bangham, Rhonda, Hall, David, Bidlingmaier, Scott, Bilgin, Metin

2001

Overview

To facilitate studies of the yeast proteome, we cloned 5800 open reading frames and overexpressed and purified their corresponding proteins. The proteins were printed onto slides at high spatial density to form a yeast proteome microarray and screened for their ability to interact with proteins and phospholipids. We identified many new calmodulin- and phospholipid-interacting proteins; a common potential binding motif was identified for many of the calmodulin-binding proteins. Thus, microarrays of an entire eukaryotic proteome can be prepared and screened for diverse biochemical activities. The microarrays can also be used to screen protein-drug interactions and to detect posttranslational modifications.

• Publisher: American Society for the Advancement of Science,American Association for the Advancement of Science,The American Association for the Advancement of Science
• Subject: Amino Acid Motifs / Amino Acid Sequence / Biological and medical sciences / Calmodulin / Calmodulin - metabolism / Calmodulin-Binding Proteins - metabolism / Cell Membrane - metabolism / Cell membranes / Cells / Cells (Biology) / Cloning / Cloning, Molecular / Eukaryotes / Fundamental and applied biological sciences. Psychology / Fungal Proteins - chemistry / Fungal Proteins - genetics / Fungal Proteins - metabolism / Genomes / Genomics / Glucose - metabolism / Glycerophospholipids / Goods and services tax / Individualized Instruction / Laboratory Equipment / Lipids / Liposomes / Liposomes - metabolism / Literary Devices / Membrane Proteins - metabolism / Microscope and microscopy / Microscopes / Microscopy / Molecular and cellular biology / Molecular Sequence Data / Moral judgment / Open Reading Frames / P branes / Peptide Library / Phosphatidylcholines - metabolism / Phosphatidylinositols - metabolism / Phospholipids / Phospholipids - metabolism / Protein Binding / Protein research / Proteins / Proteome / proteome microarrays / Proteomes / Recombinant Fusion Proteins - metabolism / Saccharomyces cerevisiae / Saccharomyces cerevisiae - genetics / Saccharomyces cerevisiae - metabolism / Signal Transduction / Streptavidin - metabolism / Yeast / Yeast (Food product) / Yeasts