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A low‐cost protocol for the optical method of vulnerability curves to calculate P 50
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A low‐cost protocol for the optical method of vulnerability curves to calculate P 50
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Journal Article
A low‐cost protocol for the optical method of vulnerability curves to calculate P 50
2025
Overview
The quantification of plant drought resistance, particularly embolism formation, within and across species, is critical for ecosystem management and agriculture. We developed a cost-effective protocol to measure the water potential at which 50% of hydraulic conductivity (
) is lost in stems, using affordable and accessible materials in comparison to the traditional optical method.
Our protocol uses inexpensive USB microscopes, which are secured along with the plants to a pegboard base to avoid movement. A Python program automatized the image acquisition. This method was applied to quantify
in an exotic species (
) and native species (
) of the Mediterranean vegetation in Baja California, Mexico.
The intra- and interspecific patterns of variation in stem
of
and
were obtained using the low-cost optical method with widely available and affordable materials that can be easily replicated for other species.
Publisher
John Wiley and Sons Inc
Subject
