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Trypsin/Lys-C protease mix for enhanced protein mass spectrometry analysis
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Journal Article
Trypsin/Lys-C protease mix for enhanced protein mass spectrometry analysis
2013
Overview
Traditionally, trypsin and Lys-C proteases are used in combination to digest proteolytically resistant proteins. Here we describe use of Trypsin/Lys-C Mix for general digestion needs. Working simultaneously under conventional non-denaturing trypsin digestion conditions, Trypsin and Lys-C enhance proteolysis and provide multiple positive effects on protein mass spectrometry analysis including increased number of identified peptides and proteins, higher analytical reproducibility and more accurate protein quantitation. Effectively, by supplementing trypsin with Lys-C, we create improved trypsin.
Publisher
Nature Publishing Group US,Nature Publishing Group
