Journal Article

Brevetoxin Dynamics and Bioavailability from Floc Following PAC-Modified Clay Treatment of Karenia brevis Blooms

Buck, Kristen N., Harlow, Samantha, Pierce, Richard, Toyoda, Jennifer H., Sibley, Christopher D., Myers, Alyssa, Anderson, Donald M., Ohnikian, Nicholas R., Freiberger, R. Ben

2025

Overview

Harmful algal blooms (HABs) caused by the dinoflagellate Karenia brevis present serious ecological and public health concerns due to the production of brevetoxins (BTX). Clay flocculation and sedimentation of cells, particularly with polyaluminum chloride (PAC)-modified clays, is a promising HAB mitigation approach. This study evaluated the efficacy of Modified Clay-II (MCII), a PAC-modified kaolinite clay, in reducing K. brevis cell abundance in mesocosm experiments and examined the bioavailability of BTX potentially released from settled floc back into the water column and sediment over the first 72 h after treatment. Additionally, we quantified trace metals in benthic clams (Mercenaria mercenaria) exposed to the floc post-treatment to assess metal accumulation and potential toxicological effects from MCII application. MCII treatment (0.2 g/L) resulted in a 91% reduction in K. brevis cell density and a 50% decrease in waterborne brevetoxins after 5 h. Brevetoxins accumulated in sediment post-flocculation, with BTX-B5 emerging as the dominant congener. Clams exposed to MCII-treated floc showed comparable tissue BTX levels to controls and significantly elevated aluminum concentrations, though without mortality. The aluminum accumulations in this study do not raise concerns for the health of the clams or the humans who eat them, given other dietary exposures. These findings support the potential of MCII for HAB mitigation while underscoring the need for further evaluation of exposure risks to all benthic species.

• Publisher: MDPI AG
• Subject: Algae / Algal blooms / Aluminum / Aluminum Hydroxide - chemistry / Animals / Bioavailability / Biological Availability / Bivalvia - metabolism / brevetoxin / Brevetoxins / Cell density / Clay / Clay - chemistry / Clay minerals / Congeners / Design of experiments / Dinoflagellates / Dinoflagellida - drug effects / Dinoflagellida - growth & development / Dinoflagellida - metabolism / Eutrophication / Evaluation / Exposure / floc / Flocculation / HABs / Harmful Algal Bloom - drug effects / harmful algal blooms / Kaolinite / Karenia brevis / Marine toxins / Marine Toxins - analysis / Marine Toxins - metabolism / Marine Toxins - toxicity / Mercenaria - drug effects / Mercenaria - metabolism / Organisms / Oxocins - analysis / Oxocins - metabolism / Oxocins - toxicity / Polyether Toxins / Public health / Salinity / Sedimentation & deposition / Sediments (Geology) / Toxins / Trace metals / Water circulation / Water column / Water quality