Journal Article

Crystal structure of the YajQ protein from Haemophilus influenzae reveals a tandem of RNP-like domains

Obmolova, Galina, Teplyakov, Alexey, Gilliland, Gary L., Bir, Nivedita, Howard, Andrew J., Reddy, Prasad

2003

Overview

A hypothetical protein encoded by the gene YajQ of Haemophilus influenzae was selected, as part of a structural genomics project, for X-ray crystallographic structure determination and analysis to assist with the functional assignment. The protein is present in most bacteria, but not in archaea or eukaryotes. The amino acid sequence has no homology to that of other proteins. The YajQ protein was cloned, expressed, and the crystal structure determined at 2.1-A resolution by applying the multiwavelength anomalous dispersion method to a mercury derivative. The polypeptide chain is folded into two domains with identical folding topology. Each domain has a four-stranded antiparallel beta-sheet flanked on one side by two alpha-helices. This structural motif is a characteristic feature of many RNA-binding proteins. The tetrameric structure observed in the crystal suggests a possibility of binding two stretches of double-stranded nucleic acid.

• Publisher: Springer Nature B.V
• Subject: Amino Acid Sequence / Archaea / Bacterial Proteins - chemistry / Bacterial Proteins - genetics / Cloning, Molecular / Consensus Sequence / Crystal structure / Crystallography, X-Ray / Crystals / Enzymes / genomics / Haemophilus influenzae / Haemophilus influenzae - chemistry / Haemophilus influenzae - genetics / Homology / Ionizing radiation / Mercury / Models, Molecular / Molecular Sequence Data / nucleic acids / Protein Conformation / Protein Folding / Protein Structure, Tertiary / Proteins / Ribonucleoproteins - chemistry / RNA-binding protein / RNA-Binding Proteins - chemistry / Structure-function relationships / Tandem Repeat Sequences