Journal Article

Detection of Passionfruit Vietnam virus (PVNV) in passionfruit by RT-PCR

Biet, Huynh Van, Toan, Truong Quang, Ngoc Ha, Tran Thi

2025

Overview

Passionfruit Woodiness Disease is caused by the Passionfruit Vietnam Virus (PVNV) and leads to significant damage in passionfruit production due to the lack of effective treatment options. Early and accurate detection of viruses in passionfruit is crucial for effective control measures. In this study, RT-PCR was established to determine the presence of PVNV in Vietnam. Primers were specifically designed to detect PVNV, yielding an amplified product of 410 bp. The optimal annealing temperature, primer concentration, and limit of detection were found to be 59°C, 0.2 µM, and 10 copies, respectively. To verify the specificity of the assay, three cDNA samples of viruses ToMV, ToMMV, and SPFMV were tested. Out of a total of nine field samples tested, PVNV was detected in which 26.47% of the samples were infected with the virus. The study has successfully established an optimal RT-PCR assay to detect the presence of PVNV.

• Publisher: IOP Publishing
• Subject: passionfruit / Polymerase chain reaction / PVNV / RT-PCR / Viruses / Woodiness disease