Asset Details
Do you wish to reserve the book?
Nucleoprotein of influenza A virus is a major target of immunodominant CD8+ T‐cell responses
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Nucleoprotein of influenza A virus is a major target of immunodominant CD8+ T‐cell responses
Do you wish to request the book?
Nucleoprotein of influenza A virus is a major target of immunodominant CD8+ T‐cell responses
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Nucleoprotein of influenza A virus is a major target of immunodominant CD8+ T‐cell responses
2013
Overview
Influenza A virus causes annual epidemics and sporadic pandemics, resulting in significant morbidity and mortality worldwide. Vaccines are currently available; however, they induce a non‐strain‐cross protective humoral immune response directed against the rapidly mutating surface glycoproteins, and thus need to be updated annually. As T cells are directed against more conserved internal influenza proteins, a T‐cell‐based vaccine has the potential to induce long‐lasting and cross‐strain protective CD8+ T‐cell immunity, and in that way minimize the severity of influenza infection. However, to rationally design such vaccines, we need to identify immunogenic T‐cell regions within the most antigenic viral proteins. In this study, we have used a systematic approach to identify immunodominant peptides in HLA‐A2‐negative donors. A broad range of CD8+ T‐cell responses were observed and 6/7 donors had an immunodominant response against the relatively conserved internal nucleoprotein (NP). Dissecting the minimal epitope regions within the immunogenic NP led to the identification of six novel immunodominant epitopes, which include a 12‐mer and an 8‐mer peptides. The majority of immunodominant epitopes was clustered within the carboxyl terminal 2/3 of the NP protein and were highly conserved. We also subjected NP to three common computer algorithms for epitope prediction and found that most of the novel epitopes would not have been predicted. Our study emphasizes the importance of using a systematic approach to identify immunodominant CD8+ T‐cell responses and suggests that the epitope‐rich regions within NP present a promising target for the T‐cell‐mediated multi‐strain influenza vaccine.
Publisher
Nature Publishing Group,Blackwell Science Ltd
