Journal Article

TFT7 Regulated the Iron Absorption System in Roots of Tomato under Iron-Deficient Conditions

Li, B., Jin, C., Wang, Y., Zhu, Y., Du, S.

2023

Overview

Iron deficiency is a major factor limiting plant growth in alkaline soils. 14-3-3 proteins are a family of phosphoserine-binding proteins. Previous studies have shown that iron deficiency significantly induces the expression of 14-3-3 protein gene TFT7 in tomatoes. In this study, a virus-induced gene silencing (VIGS) system composed of pTRV1 and pTRV2 was used to investigate whether the induction of TFT7 expression was involved in regulating the iron uptake system in tomato roots. Co-inoculation with the modified viral vectors pTRV1 and pTRV2-TFT7 effectively silenced the expression of TFT7 in tomato roots. Simultaneously, due to TFT7 silencing, expressions of the transcription factor gene SlFER , ferric chelate reductase SlFRO1 , and iron transporter gene SlIRT1 were significantly inhibited in iron-deficient roots. Additionally, TFT7 silencing significantly reduced ferric chelate reductase activity in iron-deficient tomato roots. Therefore, this study suggested that TFT7 is important for regulating the iron absorption system during iron deficient periods.

• Publisher: Pleiades Publishing,Springer Nature B.V
• Subject: 14-3-3 protein / Absorption / Alkaline soils / Biomedical and Life Sciences / Chelates / Cloning / E coli / Expression vectors / family / ferric chelate reductases / Gene expression / Gene silencing / genes / Inoculation / Iron / iron absorption / Iron deficiency / Life Sciences / Nutrient deficiency / Nutrition research / Phosphoserine / Physiology / Plant growth / Plant Physiology / Plant Sciences / Plasmids / Proteins / Reductases / Research Papers / RNA polymerase / Roots / Tomatoes / transcription factors / Variance analysis / Vectors (Biology)