Journal Article

Comparative analysis of the application efficiency of gold nanoparticles-based SERS in four foodborne pathogenic microbes

Gao, Fengtao, Ning, Xue, Chen, Niannian, Liu, Hourong

2024

Overview

It has been widely reported that SERS can detect foodborne pathogenic bacteria, but SERS has only been limited to the detection of purified single bacteria. However, there’s few single bacterial infections in the natural state were found at the most time, and rare comparative investigations of different Gram-negative and Gram-positive bacteria by SERS exist. At the present study, we are aiming at the comparative analysis of the detection efficiency toward different foodborne pathogenic microbes by a conventional citrate-reduced gold colloid, including Escherichia coli, Salmonella enteritidis, Staphylococcus aureus and Listeria monocytogenes. The SERS spectra of samples were collected with sterile 0.5% NaCl solution as aggregating compound. First, the stability of each bacterial were detected with gold nanoparticles. Afterward, SERS signals of the four bacteria were compared at different concentrations, and it was found that Gram-positive and Gram-negative bacteria have different minimum sensitivity and detection stability. Next, the mixed bacterial SERS detection was applied to classify and identify the similar Raman spectra. Spectral data were analyzed using multivariate data analysis methods, including principal component analysis (PCA) and hierarchical cluster analysis (HCA). The SERS procedure we proposed, which utilized gold nanoparticles adsorbing on bacteria and multivariate analysis, allowed differentiation of species. The results of this pilot study demonstrate the potential of SERS to rapidly identify Gram-negative and Gram-positive foodborne pathogens.Article HighlightsComparative analysis of the detection sensitivity toward different foodborne pathogenic microbes.SERS signal might be interrupted by the different structure of bacterial wall.The mixed bacterial liquid could also be effectively distinguished by SERS detection.

• Publisher: Springer Nature B.V
• Subject: Bacteria / Bacterial diseases / Bacterial infections / Cluster analysis / Comparative analysis / Data analysis / Disease / E coli / Food safety / Foodborne diseases / Foodborne pathogens / Fruits / Gold / Gram-negative bacteria / Gram-positive bacteria / Listeria / Methods / Microorganisms / Multivariate analysis / Nanoparticles / Pathogens / Principal components analysis / Public health / Raman spectra / Raman spectroscopy / Reagents / Sodium chloride / Spectrum analysis / Stability / Zoonoses