Asset Details
Do you wish to reserve the book?
Expression of Helicobacter pylori ggt Gene in Baculovirus Expression System and Activity Analysis of its Products
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Expression of Helicobacter pylori ggt Gene in Baculovirus Expression System and Activity Analysis of its Products
Do you wish to request the book?
Expression of Helicobacter pylori ggt Gene in Baculovirus Expression System and Activity Analysis of its Products
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Expression of Helicobacter pylori ggt Gene in Baculovirus Expression System and Activity Analysis of its Products
2011
Overview
The gamma-glutamyltranspeptidase (GGT) of Helicobacter pylori (HpGT) is a newly found virulence factor. In an approach to gain insight into the gene function, the four domains of the HpGT were cloned and expressed in baculovirus expression system. The results of a functional assay showed that the HpGT products acted as GGT, even when the N-terminal 380 amino acids were deleted. However, only the full length open reading frame (ORF) of the HpGT gene was apparently effective on cell growth. This result indicated that the products of the full length ORF might have an important role in gastric carcinogenesis. In this paper, we are the first to report that changes of mitochondrial membrane potential can be detected using 5, 5', 6, 6'-tetrachloro-1, 1', 3, 3'-tetraethylbenzimidazole carbocyanine iodide (JC-1) staining in insect cells.
Publisher
De Gruyter Brill Sp. z o.o., Paradigm Publishing Services
Subject
/ Cell Survival - drug effects
/ gamma-Glutamyltransferase - analysis
/ gamma-Glutamyltransferase - genetics
/ gamma-Glutamyltransferase - metabolism
/ gamma-Glutamyltransferase - pharmacology
/ Helicobacter pylori - enzymology
/ Helicobacter pylori - genetics
/ Humans
/ Membrane Potential, Mitochondrial - drug effects
