Journal Article

Enhancing Electron Transfer and Stability of Screen-Printed Carbon Electrodes Modified with AgNP-Reduced Graphene Oxide Nanocomposite

Van Pham Chung, Nguyen, Thanh Thuy, Tran, Quang Huy, Nguyen Thi Hue, Phan, Vu Ngoc, Tonezzer Matteo, Ngo, Xuan Dinh, Dang Thanh Le Thi, Nguyen Tien Khi, Anh-Tuan, Le, Vu Quang Khue, Nguyen Thi Luyen

2022

Overview

This paper presents a reliable solution to enhance the electron transfer and stability of screen-printed carbon electrodes (SPCEs) for the direct detection of pathogenic bacteria. A nanocomposite of silver nanoparticles (AgNPs) and reduced graphene oxide (rGO) was used to modify the SPCEs. Herein, the nanocomposite was synthesized via a hydrothermal method and then characterized by physicochemical methods. The electron transfer rate and electrochemical properties of the AgNP-rGO nanocomposite-modified SPCEs were investigated using cyclic voltammetry (CV) and electrochemical impedance spectroscopy. Measurements were performed for the detection of Salmonella bacteria without any labels. Results showed that the nanocomposite firmly adhered to the surfaces of the SPCEs, led to an increase of approximately 160% in the peak current, and decreased the charge transfer resistance to 0.45 kΩ. Electrochemical stability was found in 30 CV cycles. The modified SPCEs could detect Salmonella bacteria directly at concentrations of 10–105 CFU/mL, with a limit of detection (LoD) of as low as 22 CFU/mL. A possible mechanism was proposed to explain the enhanced electron transfer on the surface and the stability of the AgNP-rGO nanocomposite-modified SPCEs. The biosensor showed high stability, cost-effectiveness, and simplicity for the direct detection of pathogenic bacteria.

• Publisher: Springer Nature B.V
• Subject: Bacteria / Biosensors / Carbon / Charge transfer / Electrochemical analysis / Electrochemical impedance spectroscopy / Electron transfer / Electrons / Graphene / Nanocomposites / Nanoparticles / Salmonella / Silver / Surface stability