Asset Details
Do you wish to reserve the book?
Identification of Inwardly Rectifying Potassium Channels in Bovine Retinal and Choroidal Endothelial Cells
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Identification of Inwardly Rectifying Potassium Channels in Bovine Retinal and Choroidal Endothelial Cells
Do you wish to request the book?
Identification of Inwardly Rectifying Potassium Channels in Bovine Retinal and Choroidal Endothelial Cells
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Identification of Inwardly Rectifying Potassium Channels in Bovine Retinal and Choroidal Endothelial Cells
2002
Overview
Ion channels were studied using the whole-cell patch clamp technique in bovine retinal and choroidal microvascular endothelial cells (MVEC) cultured under the same conditions. The two types of MVEC expressed inward currents at hyperpolarizing voltage steps and showed small outward currents at depolarizing steps. The extrapolated reversal potentials of the inward currents were near to the potassium equilibrium potential. Cs + and the K + channel blocker TEA reduced the amplitudes of the currents indicating the selectivity and permeability for potassium. This was confirmed by changes of outside K + concentration shifting the I-V curves to the right. RT-PCR studies revealed the presence of mRNA of Kir2.1, an inwardly rectifying K + channel, in retinal and choroidal MVEC. The profile of the small outward currents is related to the Kv family but not identical with the Kv1.4 subtype.
Publisher
S. Karger AG
Subject
/ Cattle
/ DNA, Complementary - analysis
/ Endothelium, Vascular - metabolism
/ Endothelium, Vascular - physiology
/ Fluorescent Antibody Technique
/ Membrane Potentials - drug effects
/ Membrane Potentials - physiology
/ Patch-Clamp Techniques - methods
/ Potassium Channels, Inwardly Rectifying - classification
/ Potassium Channels, Inwardly Rectifying - genetics
/ Potassium Channels, Inwardly Rectifying - isolation & purification
/ Potassium Channels, Inwardly Rectifying - physiology
