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Construction of high density genetic map and QTL mapping in sorghum × sudangrass
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Construction of high density genetic map and QTL mapping in sorghum × sudangrass
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Construction of high density genetic map and QTL mapping in sorghum × sudangrass
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Journal Article
Construction of high density genetic map and QTL mapping in sorghum × sudangrass
2021
Overview
Understanding the genetic basis for forage yield traits is essential for forage sorghum and sudangrass hybrid breeding. In this study, we constructed a high-density genetic map using a recombinant inbred line population between sorghum Tx623A and sudangrass Sa using RAD-seq and performed QTL mapping for plant height (PH), stem diameter (SD), tiller number (TN), total shoot fresh weight (FW) and total shoot dry weight (DW). The map consists of 1,065 markers with a total length of 1191.7 cM. A total of 43 QTLs were detected, including three QTLs for PH, eight for SD, ten for TN, ten for FW and twelve for DW. Two QTL clusters contained overlapping QTLs for TN, FW and DW. One cluster (qTN6.2/qFW6/qDW6.1) also overlaps with qTN6.1 and qDW6.2. The sorghum ortholog of a known heterotic biomass gene ARGOS, Sobic.006G092500, was located within all the five QTLs mapped on chromosome 6. Our results showed that a high-density genetic map is useful for dissecting important traits and identifying underlying genes in sorghum and sudangrass hybrid populations.
Publisher
Springer Nature B.V
Subject
